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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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PPAR (alpha, delta, gamma) Transcription Factor Assay Kit (ab133113)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (4)

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Transcription Factor Binding Assay for PPAR gamma
  • Transcription Factor Binding Assay for PPAR delta
  • Transcription Factor Binding Assay for PPAR alpha

Key features and details

  • Assay type: Semi-quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Adherent cells, Nuclear Extracts, Suspension cells

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Overview

  • Product name

    PPAR (alpha, delta, gamma) Transcription Factor Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells, Suspension cells, Nuclear Extracts
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mammals
  • Product overview

    Abcam's PPAR Transcription Factor Assay Kit (ab133113) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.


    A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the PPAR response element is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPAR response element. PPAR alpha, delta, or gamma are detected by addition of specific primary antibodies directed against the individual PPARs. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133113 comes with a single plate that measures all three isoforms of PPAR alpha, delta, and gamma. There are enough reagents for one-third of a plate for each isoform.

  • Notes

    Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands. PPARs play pivotal roles in the regulation of lipid metabolism and homeostasis and are important indirect and direct regulators of cellular insulin sensitivity.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 96 tests
    96-Well Plate Cover 1 unit
    Polysorbate 20 1 vial
    Transcription Factor Antibody Binding Buffer (10X) 1 x 3ml
    Transcription Factor Binding Assay Buffer (4X) 1 x 3ml
    Transcription Factor Complete PPARα Positive Control 1 vial
    Transcription Factor Complete PPARα Primary Antibody 1 vial
    Transcription Factor Complete PPARδ Positive Control 1 vial
    Transcription Factor Complete PPARδ Primary Antibody 1 vial
    Transcription Factor Complete PPARγ Positive Control 1 vial
    Transcription Factor Complete PPARγ Primary Antibody 1 vial
    Transcription Factor Developing Solution 1 x 12ml
    Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 x 100µl
    Transcription Factor PPAR 96-Well Strip Plate 1 unit
    Transcription Factor PPAR Competitor dsDNA 1 vial
    Transcription Factor Reagent A 1 x 120µl
    Transcription Factor Stop Solution 1 x 12ml
    Wash Buffer Concentrate (400X) 1 x 5ml
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Lipid Metabolism Kits
    • Fatty acids
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Lipid Metabolism Kits
    • Obesity
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Kits/ Lysates/ Other
    • Kits
    • Epigenetic kits
    • Transcription factor assay kits
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation
  • Cellular localization

    Cytoplasm, nucleus.
  • Alternative names

    • CIMT1
    • FAAR
    • GLM1
    • hPPAR
    • NR1C3
    • NR1CI
    • NRIC2
    • NUC1
    • NUCI
    • NUCII
    • Peroxisome proliferator-activated receptor alpha
    • Peroxisome proliferator-activated receptor beta
    • Peroxisome proliferator-activated receptor delta
    • PPARalpha
    • PPARB
    • PPARG
    • PPARG1
    • PPARG2
    • PPARGgamma
    see all
  • Database links

    • Entrez Gene: 5465 Human
    • Entrez Gene: 5467 Human
    • Entrez Gene: 5468 Human
    • Omim: 600409 Human
    • Omim: 170998 Human
    • SwissProt: Q07869 Human
    • SwissProt: P37231 Human
    • SwissProt: Q03181 Human

    Associated products

    • Related Products

      • Indomethacin, Non-selective COX inhibitor (ab120719)
      • Rosiglitazone, PPARgamma agonist (ab120762)
      • Pioglitazone hydrochloride, PPAR-gamma agonist (ab120794)
      • Telmisartan, angiotensin II (AT1) antagonist (ab120831)
      • Clofibric acid, PPARalpha agonist (ab120833)
      • GW 9662, PPARgamma antagonist (ab141125)
      • Ciglitazone, PPARgamma agonist (ab141139)
      • MK886, Lipoxygenase inhibitor (ab141140)
      • 15-Deoxy-Delta12,14-prostaglandin J2, Selective PPARgamma agonist (ab141717)
      • Nuclear Extraction Kit (ab221978)

    Images

    • Transcription Factor Binding Assay for PPAR gamma
      Transcription Factor Binding Assay for PPAR gamma
      Increasing amounts of positive control (total lysate) are assayed for PPAR gamma DNA-binding activity using ab133113.
    • Transcription Factor Binding Assay for PPAR delta
      Transcription Factor Binding Assay for PPAR delta
      Increasing amounts of positive control (total lysate) are assayed for PPAR delta DNA-binding activity using ab133113.
    • Transcription Factor Binding Assay for PPAR alpha
      Transcription Factor Binding Assay for PPAR alpha
      Increasing amounts of positive control (total lysate) are assayed for PPAR alpha DNA-binding activity using ab133113.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab133113? Please let us know so that we can cite the reference in this datasheet.

    ab133113 has been referenced in 4 publications.

    • Jo DS  et al. Loss of HSPA9 induces peroxisomal degradation by increasing pexophagy. Autophagy 16:1989-2003 (2020). PubMed: 31964216
    • Kasonga A  et al. Activation of PPARs Modulates Signalling Pathways and Expression of Regulatory Genes in Osteoclasts Derived from Human CD14+ Monocytes. Int J Mol Sci 20:N/A (2019). PubMed: 30979019
    • Zhang Q  et al. The interleukin-4/PPAR? signaling axis promotes oligodendrocyte differentiation and remyelination after brain injury. PLoS Biol 17:e3000330 (2019). PubMed: 31226122
    • Meex RC  et al. ATGL-mediated triglyceride turnover and the regulation of mitochondrial capacity in skeletal muscle. Am J Physiol Endocrinol Metab 308:E960-70 (2015). Functional Studies . PubMed: 25852007

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I am interested in the PPAR alpha/delta/gamma transcription factor assay kit. I see the kit has alpha and gamma positive controls but no delta controls. Is this a mistake or was a delta pos control intentionally left out?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 21 2013

    Answer

    There is a control protein for PARP delta included with this kit. Due to an IT issue this component did not make it on to the online product datasheet. This should be updated shortly.

    Read More

    Abcam Scientific Support

    Answered on Feb 21 2013

    Question

    We need tp know the actual volume of Sulphuric acid

    Read More

    Abcam community

    Verified customer

    Asked on Jan 03 2013

    Answer


    According to the datasheet, the transcription factor stop solution is the only component to contain sulphuric acid.
    I can confirm that the transcription factor stop solution contains 12 ml of a 0.5 M sulphuric acid solution.

    Read More

    Abcam Scientific Support

    Answered on Jan 03 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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