Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

SREBP-1 Transcription Factor Assay Kit (ab133125)

Submit a review Q&A (2)References (6)
ELISA ab133125 SREBP-1 Transcription Factor Assay Kit
  • ELISA ab133125 SREBP-1 Transcription Factor Assay Kit
  • Functional Studies - SREBP-1 Transcription Factor Assay Kit (ab133125)
  • Functional Studies - SREBP-1 Transcription Factor Assay Kit (ab133125)
  • Functional Studies - SREBP-1 Transcription Factor Assay Kit (ab133125)

Key features and details

  • Assay type: Semi-quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Cell culture extracts, Nuclear Extracts

Overview

  • Product name

    SREBP-1 Transcription Factor Assay Kit

  • Detection method

    Colorimetric

  • Sample type

    Cell culture extracts, Nuclear Extracts

  • Assay type

    Semi-quantitative

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Product overview

    Abcam's SREBP-1 Transcription Factor Assay (ab133125) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

    A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized onto the wells of a 96-well plate. SREBP-1 contained in a nuclear extract binds specifically to the SREBP response element. SREBP-1 is detected by addition of a specific primary antibody directed against SREBP-1. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. The SREBP-1 Transcription Factor Assay detects human, rat, and murine SREBP-1.

  • Notes

    Lipid homeostasis in vertebrate cells is regulated by a family of transcription factors called sterol regulatory elements binding proteins (SREBPs). SREBPs directly activate the expression of over 30 gened involved in teh synthesis and uptake of cholesterol, fatty cids, triglycerides and phopholipids. SREBP-1 acts primarily to activate genes in fatty acid synthesis. Nuclear SREBP-1 upregulates gene expression of a group of a target lipogenic enzymes such as acetyl CoA carboxylase and fatty acid synthase.

  • Platform

    Microplate reader

Properties

Associated products

Images

  • ELISA ab133125 SREBP-1 Transcription Factor Assay Kit
    ELISA ab133125 SREBP-1 Transcription Factor Assay Kit

    HepG2 cells were grown in protein free hybridoma medium overnight (Life Technologies), followed by a 30 minutes incubation with the addition of 1% (2-Hydroxypropyl)-β-cyclodextrin (HPBC; Sigma) or with the supplement of liquid cholesterol (Life Technologies). 10 uL of nuclear extracts (ab113474) were tested, corresponding to 2.5e5 cells per well.

  • ELISA ab133125 SREBP-1 Transcription Factor Assay Kit
    ELISA ab133125 SREBP-1 Transcription Factor Assay Kit

    Positive control (10 or 5 uL) with (10 or 2 uL) or without inhibitor (duplicates; +/- SD).

  • Functional Studies - SREBP-1 Transcription Factor Assay Kit (ab133125)
    Functional Studies - SREBP-1 Transcription Factor Assay Kit (ab133125)
    Comparison of Abcam's SREBP-1 Transcription Factor Assay Kit (ab133125) and SREBP-2 Transcription Factor Assay Kit (ab133114) specificity. The results demonstrate little to no cross reactivity of SREBPs in the opposing assay. Cross Reactivity: (+) SREBP-1a, SREBP-1b, and SREBP-1c
  • Functional Studies - SREBP-1 Transcription Factor Assay Kit (ab133125)
    Functional Studies - SREBP-1 Transcription Factor Assay Kit (ab133125)
    Measurement of SREBP-1 activity in HeLa (squares) and HepG2 (dots) cell lysates.
  • Functional Studies - SREBP-1 Transcription Factor Assay Kit (ab133125)
    Functional Studies - SREBP-1 Transcription Factor Assay Kit (ab133125)
    Quantification of recombinant SREBP-1 from E. coli cell lysates.

References (6)

Publishing research using ab133125? Please let us know so that we can cite the reference in this datasheet.

ab133125 has been referenced in 6 publications.

  • Pham DV & Park PH Adiponectin triggers breast cancer cell death via fatty acid metabolic reprogramming. J Exp Clin Cancer Res 41:9 (2022). PubMed: 34986886
  • Yang Y  et al. LncRNA NEAT1 Potentiates SREBP2 Activity to Promote Inflammatory Macrophage Activation and Limit Hantaan Virus Propagation. Front Microbiol 13:849020 (2022). PubMed: 35495674
  • Zhou J  et al. The Role of PPARd Agosnist GW501516 in Rats with Gestational Diabetes Mellitus. Diabetes Metab Syndr Obes 13:2307-2316 (2020). PubMed: 32669864
  • Chen X  et al. LncRNA NEAT1 promotes hepatic lipid accumulation via regulating miR-146a-5p/ROCK1 in nonalcoholic fatty liver disease. Life Sci 235:116829 (2019). PubMed: 31484042
  • Marwarha G  et al. Palmitate-Induced SREBP1 Expression and Activation Underlies the Increased BACE 1 Activity and Amyloid Beta Genesis. Mol Neurobiol N/A:N/A (2018). PubMed: 30569418
  • Salomone F  et al. Silibinin Restores NAD? Levels and Induces the SIRT1/AMPK Pathway in Non-Alcoholic Fatty Liver. Nutrients 9:N/A (2017). PubMed: 28973994

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

I was looking at this assay kit and was wondering if the primary antibody recognizes which part of the protein (Protein as whole or only the nuclear signal). Is this antibody monoclonal or polyclonal? Can you give us the DNA sequence that is coated on the plate, as I would like to rationalize my study after I intend to use it. Also, Please let me know the isoform (full length or nuclear signal) of SREBP1 that was expressed in E.coli. Your graphs for HepG2 cells and HeLa cells say cell lysates, does it mean nuclear/whole cell? Is the competitor ds DNA same as the one that is coated on the plate?

Read More
Answer

The antibody supplied in the SREBP-1 Transcription Factor Assay Kit is polyclonal. We have not mapped which portions of SREBP-1 it detects. The positive control supplied is a clarified cell lysate, so we expect it will contain a mixture of SREBP-1 forms. The antibody in this kit will detect the SREBP-1 that is bound to the immobilized DNA, so if the SREBP-1 is not able to bind to the DNA, it will not be detected in this assay.

Read More

Question

1. What DNA sequence is coated on the plate?
2. What DNA sequence is used as the competitor?
3. Are whole cell lysates or nuclear lysates used from HepG2 and HeLa cells?

Read More
Answer

1. The plate is coated with the dsDNA consensus sequence specific for SREBP. The sequence is 5'TCACCTGA-3'.
2. The competitor DNA sequence is 5'TCACCTGA'.
3. The HepG2 and HeLa lysates are whole cell lysates.




I hope this is helpful. Please contact me again if you have any further questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com