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Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit (ab108915)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (2)

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Indirect ELISA - Urokinase type plasminogen activator Human Chromogenic Activity Assay Kit (ab108915)
  • Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)
  • Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Cell culture supernatant, Plasma
  • Sensitivity: 0.0053 U/ml

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Overview

  • Product name

    Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit
    See all Urokinase kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    0.0053 U/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.


    ab108915 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma and cell culture supernatants. The assay measures the ability of uPA to activate the plasminogen to plasmin in coupled or indirect assays that contain uPA, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the uPA enzymatic activity.


     


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Assay Diluent 1 x 30ml
    Human Plasminogen 1 x 2 vials
    Human uPA Standard 1 vial
    Microplate 1 unit
    Plasmin Substrate 1 x 2 vials
    Sealing Tapes 3 units
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Proteases
    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Coagulation Activity Assays
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Neuroscience
    • Processes
  • Function

    Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificity

    Expressed in the prostate gland and prostate cancers.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P00749 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • ATF
    • ATF uPA
    • BDPLT5
    • Plasminogen activator
    • Plasminogen activator urinary
    • Plasminogen activator urokinase
    • PLAU
    • QPD
    • u PA
    • U plasminogen activator
    • u-PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase plasminogen activator
    • Urokinase type plasminogen activator
    • Urokinase type plasminogen activator precursor
    • Urokinase-type plasminogen activator chain B
    see all
  • Database links

    • Entrez Gene: 5328 Human
    • Omim: 191840 Human
    • SwissProt: P00749 Human
    • Unigene: 77274 Human

    Images

    • Indirect ELISA - Urokinase type plasminogen activator Human Chromogenic Activity Assay Kit (ab108915)
      Indirect ELISA - Urokinase type plasminogen activator Human Chromogenic Activity Assay Kit (ab108915)

      uPA measured in biologicals showing quantity (IU) per mL of sample tested.

    • Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)
      Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)

      uPA Chromogenic Activity Standard Curve (For High uPA Activity Samples)

    • Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)
      Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)

      uPA Chromogenic Activity Standard Curve (For Low uPA Activity Samples)

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab108915? Please let us know so that we can cite the reference in this datasheet.

    ab108915 has been referenced in 2 publications.

    • Hohensinner PJ  et al. PAI-1 (Plasminogen Activator Inhibitor-1) Expression Renders Alternatively Activated Human Macrophages Proteolytically Quiescent. Arterioscler Thromb Vasc Biol N/A:N/A (2017). PubMed: 28818858
    • Gupta V  et al. Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin. Sci Rep 7:8412 (2017). PubMed: 28827627

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I have one other question regarding the specificity of this assay. Since tissue associated plasminogen activator (tPA) can also convert plasminogen to plasmin, how can I be sure that I am measuring only uPA-mediated conversion of plasminogen to plasmin and not tPA-mediated conversion? It seems to me that what I will be measuring is the overall conversion of plasminogen to plasmin and not a uPA-specific conversion. Can you clarify this?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 03 2015

    Answer



    For uPA activity kits, we have two types of kit. One is ab108916 which is direct using uPA substrate to generate the color. The uPA substrate is specifically designed for its specificity for uPA. This assay can differentiate uPA activity vs. tPA activity.

    On the other hand, we also have an indirect uPA activity kit which is ab108915. This kit is using a plasmin substrate to measure uPA activity.

    ab108916 can differentiate uPA vs. tPA activity, however, it is not as sensitive as ab108915 which is an indirect assay. ab108915 is more sensitive assay but is no able to differentiate uPA vs. tPA activity. We provide uPA standard for indirect assay is for reference of uPA activity.

    If a cell culture sample specific for uPA expression is used, ab108915 will works well. However, if a plasma or serum sample is used, it is difficult to differentiate from tPA activity. But as the uPA activity accounts for at least 50 % of activity for cleave plasminogen to plasmin, the kit can at least provide a reference for uPA activity when samples are compared although we would recommend ab108916 if the samples have enough uPA.

    Click here (or use the following: https://www.abcam.com/Urokinase-type-Plasminogen-Activator-Human-Chromogenic-Activity-Assay-Kit-ab108915.html).

    Click here (or use the following: https://www.abcam.com/Urokinase-type-Plasminogen-Activator-Human-Chromogenic-Activity-Assay-Kit-Direct-Assay-ab108916.html).

    Read More

    Anja Hoffmann

    Abcam Scientific Support

    Answered on Mar 03 2015

    Question

    Dear,

    I'm interested in the ab108915 assay kit. However, I need more information on the kit contents to be able to decide if it is suited for my application. More specifically it is important to know what the pH is of the assay diluent. I can't find this information in the protocol or the SDS. It would be great if you can let me know.

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 30 2012

    Answer

    Thank you for your enquiry.

    I can confirm the pH for the assay diluent buffer is 8,5.

    I hope this will be helpful. If you require any further details or information, please donot hesitate to let me know.

    Read More

    Abcam Scientific Support

    Answered on Aug 30 2012

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