Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit (ab108915)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Plasma
- Sensitivity: 0.0053 U/ml
Overview
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Product name
Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit
See all Urokinase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Enzyme activity (quantitative) -
Sensitivity
0.0053 U/ml -
Species reactivity
Reacts with: Human -
Product overview
Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.
ab108915 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma and cell culture supernatants. The assay measures the ability of uPA to activate the plasminogen to plasmin in coupled or indirect assays that contain uPA, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the uPA enzymatic activity.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Diluent 1 x 30ml Human Plasminogen 1 x 2 vials Human uPA Standard 1 vial Microplate 1 unit Plasmin Substrate 1 x 2 vials Sealing Tapes 3 units -
Research areas
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Function
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
Tissue specificity
Expressed in the prostate gland and prostate cancers. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsPhosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ATF
- ATF uPA
- BDPLT5
see all -
Database links
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
Images
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Indirect ELISA - Urokinase type plasminogen activator Human Chromogenic Activity Assay Kit (ab108915)
uPA measured in biologicals showing quantity (IU) per mL of sample tested.
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Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)uPA Chromogenic Activity Standard Curve (For High uPA Activity Samples)
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Indirect ELISA - Urokinase type plasminogen activator Human chromogenic activity assay kit example standard curve (ab108915)uPA Chromogenic Activity Standard Curve (For Low uPA Activity Samples)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab108915 has been referenced in 2 publications.
- Hohensinner PJ et al. PAI-1 (Plasminogen Activator Inhibitor-1) Expression Renders Alternatively Activated Human Macrophages Proteolytically Quiescent. Arterioscler Thromb Vasc Biol N/A:N/A (2017). PubMed: 28818858
- Gupta V et al. Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin. Sci Rep 7:8412 (2017). PubMed: 28827627