Antibody Purification Kit (Protein G) (ab128747)

The antibody purification kits that we carry, such as ab128745 and ab128747, will only recognize and purify IgG. For other classes such as IgA, IgD, and IgM, we have class-specific antibodies, for instance ab124716, which could be used to immunprecipitate those classes of immunoglobulin. Please let me know if you have any questions.

As we discussed, our studies have found little effect on the conjugation chemistry with up to 0.05% BSA and up to 0.1% glycine in the antibody storage buffer. Thus, I think you do not have to worry about those components. However, as you know the 0.01% sodium azide will inhibit HRP activity, and as you mentioned the 50% glycerol is making it hard to remove the sodium azide.

Antibody purification kit if you are going to stay with ab76424:
Protein A for rabbit antibodies ab102784: https://www.abcam.com/Antibody-Purification-Kit-Protein-A-ab102784.html
Protein G for other species ab 128747: https://www.abcam.com/Antibody-Purification-Kit-Protein-G-ab128747.html
For mouse antibodies ab128745: https://www.abcam.com/Mouse-Antibody-Purification-Kit-ab128745.html

Previous customers have contacted us with issues purifying antibodies with high concentrations of glycerol, and we found the following solution proved successful:
" When using the antibody purification kit the presence of glycerol in the antibody buffer will make it more viscous. This means that it will require more G force to pass through the spin column. It is possible to buffer exchange the antibody it just means the customer will have to spin for much longer. Alternatively, a simpler solution would be to dilute the antibody. This would reduce the glycerol’s concentration, which in turn will make the buffer less viscous and easier to spin out. You can then use the same spin column to concentrate the antibody back to its original concentration and volume, after having performed the buffer exchange."

Additionally, we have our EasyLink conjugation kits, including HRP conjugation, listed through the link below. Our EasyLink conjugation kits are capable of fully successful conjugation with glycerol concentrations of up to 50%. We are currently running a promotion for an extra 20% off our EasyLink kits:
EasyLink Kits: https://www.abcam.com/index.html?pageconfig=resource&rid=13148#enzymatic
Easylink Promotion:https://www.abcam.com/index.html?pageconfig=resource&rid=15389&source=pagetrap&viapagetrap=easylink123
Below is a list of compatible and incompatible buffers with our EasyLink conjugation reactions:
https://www.abcam.com/index.html?pageconfig=resource&rid=13156#11

Thank you for contacting us.

I have been in contact with the originator of these products. Unfortunately they do not have any direct experience doing these types of procedures with pre-conjugated products and cannot provide any further information.

My colleagues and I believe that these procedures should be effective for removal of unwanted buffer additives from the antibody however we would likely recommend using the antibody concentration kit as it seems less likely that damage may occur. Our concentration kits may be found at:

1 column:

https://www.abcam.com/Antibody-Concentration-Kit-1-column-ab102776.html

3 columns:

https://www.abcam.com/Antibody-Concentration-Kit-3-columns-ab102778.html

Please remember that these products are covered by our Abpromise guarantee. We are happy to provide scientific support, replacement or refund should a product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for contacting us.

We currently do not offer ab8580 in a buffer which is free of BSA. When using our EasyLink Conjugation Kits concentrations of up to 0.5% BSA and 0.1% gelatin have little effect on the conjugation chemistry. As such I would suggest one of the following options.

1) Purify the antibody to remove the BSA and Sodium Azide. We carry antibody purification kits which canquickly remove these contaminants. Our Protein G purification kit may be found here:https://www.abcam.com/Antibody-Purification-Kit-Protein-G-ab128747.html

2) Use another anti Histone H3 K4me3 antibody which does not contain BSA or other additive at levels which will interfere with your conjugation.AB12209 contains only 0.2% Sodium Azide and no BSA.https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam12209-ChIP-Grade-ab12209.html

More information and FAQs about EasyLink Conjugation Kits may be found at these links:

FAQs: https://www.abcam.com/index.html?pageconfig=resource&rid=13156

EasyLink:https://www.abcam.com/index.html?pageconfig=resource&rid=13148

We are currently running a promotion through August for 25% off of any EasyLink conjugation kit by using the codeNEW-F76ZBby 31 August 2012.

As always, these products are covered by our Abpromise guarantee. We provide scientific support, replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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