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    products/conjugation-kits/b-phycoerythrin-conjugation-kit-lightning-link-ab102871.pdf

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B-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102871)

  • Datasheet
  • SDS
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Conjugation - Phycoerythrin Conjugation Kit  (ab102871)

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    Overview

    • Product name

      B-Phycoerythrin Conjugation Kit - Lightning-Link®
    • Product overview

      B-Phycoerythrin Conjugation Kit ab102871 uses a simple and quick process  for B-PE labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.


      To conjugate an antibody to B-Phycoerythrin using this kit:
      - add modifier to antibody and incubate for 3 hrs
      - add quencher and incubate for 30 mins
      The B-PE conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


      Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to B-Phycoerythrin.


      Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

    • Notes

      This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® B-PE Labeling Kit. 716-0015 is the same as the 600 µg size. 716-0010 is the same as the 3 x 60 ug size. 716-0005 is the same as the 60 µg size.

      Amount and volume of antibody for conjugation to B-Phycoerythrin.

       Kit size Recommended 
      amount of antibody
       
      Maximum antibody 
      volume1
      3 x 60 µg 3 x 60 µg 3 x 60 µL
      60 µg 1 x 60 µg  1 x 60 µL
      600 µg 1 x 600 µg 1 x 600 µL

      1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

      The selling size of this product has been changed – it is now based on the amount of antibody that can be conjugated with the kit, not the amount of PE mix provided. The quantity of reagents provided have not changed or the amount of antibody that can be conjugated.

       

      Buffer Requirements for Conjugation

      Buffer should be pH 6.5-8.5.

      Compatible buffer constituents 
      If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

      50mM / 0.6% Tris1 0.1% BSA 50% glycerol
      0.1% sodium azide PBS Potassium phosphate
      Sodium chloride HEPES Sucrose
      Sodium citrate EDTA Trehalose

      1 Tris buffered saline is almost always ≤ 50 mM / 0.6%


      Incompatible buffer constituents

      Thiomerosal Proclin Glycine
      Arginine Glutathione DTT

      If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

      Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

      Storing and handling conjugation kits

      Lyophilized Lightning-Link® components are hygroscopic.

      Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 60 µg 600 µg 3 x 60 µg
      ab274139 - BPE mix 1 x 60µg 1 x 600µg 3 x 60µg
      Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl
      Quencher reagent 1 x 200µl 1 x 200µl 1 x 200µl
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • Conjugates
      • Kits/ Lysates/ Other
      • Kits
      • Conjugation Kits
      • Phycoerythrin
    • Alternative names

      • B PE
      • PE

    Associated products

    • Related Products

      • Antibody Concentration And Clean-Up Kit (ab102778)
      • Antibody Purification Kit (Protein A) (ab102784)

    Images

    • Conjugation - Phycoerythrin Conjugation Kit  (ab102871)
      Conjugation - Phycoerythrin Conjugation Kit (ab102871)

      This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

    Protocols

    • Protocol Booklet - Chinese Version
    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab102871? Please let us know so that we can cite the reference in this datasheet.

    ab102871 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    We are involved in a study that uses bone marrow stromal cells from goat (BMSC), and we’d need to characterize the cells, by means of flow citometry, against CD-34 and CD-90. Our difficulty is to find a specific antibody, that reacts positively with goat cells. I’ve searcher on several suppliers and only on yours I found a CD-34 with positive reactivity against sheep. Do you know if someone has used it against goat, as goat BMSC are being used on several studies?

    Would you recommend this antibody: Anti-CD34 antibody [EP373Y] (ab81289), to be used against goat?

    We also would like to mark the goat BMSCs cells against the CD-90. In your site I did not see a reference to positive reactivity against goat, sheep or cow. Do you know if anyone has used one of the listed antibodies for goat studies, and if they have shown positive reactivity?

    In case of a positive answer which one would you recommend us?

    In the case that abcam can supply us with this two antibodies, we would like them marked with fluorescent probes (not FICT or PE, as we’ll combine this antibodies with others we’ve bought a few months ago and that are marked with PE and FICT), which are the possibilities that you offer?

    Do you have a dealer in Portugal? Frilabo or Alfagene?

    I really would appreciate if you could help us with this question

    Thank you very much for your time

    Yours sincerely

    Read More

    Abcam community

    Verified customer

    Asked on Feb 06 2012

    Answer

    Thank you for contacting us.

    We currently do not have any publication information regarding its use in sheep BMSCs. I am sorry we can't offer any tested data. This antibody however was tested with sheep lysates and gave good staining so it is more likely that the antibody with detect sheep CD34 target. The use of ab81289 (https://www.abcam.com/CD34-antibody-EP373Y-ab81289.html) comes with Abcam Abpromise guarantee which states we will refund your money if the antibody fails to detect the CD34 target.

    https://www.abcam.com/index.html?pageconfig=abpromise

    I can provide further opinion about CD90 antibodies could you send me goat CD90 protein sequence?

    We have kits that can be used for conjugation of antibodies. Please check the following products

    ab102884; https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-100µg-FITC-ab102884.html
    ab102883; https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-1-x-1mg-FITC-ab102883.html

    ab102870; https://www.abcam.com/EasyLink-B-Phycoerythrin-Conjugation-Kit-1-x-100µg-B-PE-ab102870.html
    ab102871; https://www.abcam.com/EasyLink-B-Phycoerythrin-Conjugation-Kit-3-x-100µg-B-PE-ab102871.html

    For full list of conjugation kits please click; https://www.abcam.com/index.html?pageconfig=resource&rid=13148

    We do not have distributor in Portugal you can buy directly from us.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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