B-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102871)
Overview
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Product name
B-Phycoerythrin Conjugation Kit - Lightning-Link® -
Product overview
B-Phycoerythrin Conjugation Kit ab102871 uses a simple and quick process for B-PE labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
To conjugate an antibody to B-Phycoerythrin using this kit:
- add modifier to antibody and incubate for 3 hrs
- add quencher and incubate for 30 mins
The B-PE conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to B-Phycoerythrin.
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
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Notes
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® B-PE Labeling Kit. 716-0015 is the same as the 600 µg size. 716-0010 is the same as the 3 x 60 ug size. 716-0005 is the same as the 60 µg size.
Amount and volume of antibody for conjugation to B-Phycoerythrin.
Kit size Recommended
amount of antibodyMaximum antibody
volume13 x 60 µg 3 x 60 µg 3 x 60 µL 60 µg 1 x 60 µg 1 x 60 µL 600 µg 1 x 600 µg 1 x 600 µL 1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
The selling size of this product has been changed – it is now based on the amount of antibody that can be conjugated with the kit, not the amount of PE mix provided. The quantity of reagents provided have not changed or the amount of antibody that can be conjugated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.50mM / 0.6% Tris1 0.1% BSA 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose 1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
Incompatible buffer constituentsThiomerosal Proclin Glycine Arginine Glutathione DTT If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 60 µg 600 µg 3 x 60 µg ab274139 - BPE mix 1 x 60µg 1 x 600µg 3 x 60µg ab274106 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl ab274296 - Quencher reagent 1 x 200µl 1 x 200µl 1 x 200µl -
Research areas
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Alternative names
- B PE
- PE
Associated products
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Related Products
Images
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Conjugation - Phycoerythrin Conjugation Kit (ab102871)
This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab102871 has not yet been referenced specifically in any publications.