For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/conjugation-kits/dylight-405-conjugation-kit-fast-lightning-link-ab201798.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits Conjugation Kits Dylight
Share by email

DyLight® 405 Conjugation Kit (Fast) - Lightning-Link® (ab201798)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

conjugation

    You may also be interested in

    Conjugation
    Product image
    Antibody Concentration And Clean-Up Kit (ab102778)
    Conjugation
    Product image
    BSA Removal Kit (ab173231)
    Conjugation
    Product image
    DyLight® 488 Conjugation Kit (Fast) - Lightning-Link® (ab201799)

    View more associated products

    Overview

    • Product name

      DyLight® 405 Conjugation Kit (Fast) - Lightning-Link®
    • Product overview

      DyLight® 405 Conjugation Kit / DyLight® 405 Labeling Kit ab201798 uses a simple and quick process for DyLight® 405 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.


      To conjugate an antibody to DyLight® 405 using this kit:
      - add modifier to antibody and incubate for 15 mins
      - add quencher and incubate for 5 mins
      The DyLight® 405 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


      Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to DyLight® 405. 


      Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

    • Notes

      This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid DyLight® 405 Labeling Kit. 321-0015 is the same as the 1 mg size. 321-0010 is the same as the 3 x 100 ug size. 321-0030 is the same as the 3 x 10 ug size. 321-0005 is the same as the 100 ug size.

      Amount and volume of antibody for conjugation to DyLight® 405

       Kit size Recommended 
      amount of antibody
      1 
      Maximum 
      amount of antibody
      Maximum antibody 
      volume2
      3 x 10 µg 3 x 10 µg  3 x 20 µg 3 x 10 µL
      100 µg 1 x 100 µg 1 x 200 µg 1 x 100 µL
      3 x 100 µg 3 x 100 µg  3 x 200 µg 3 x 100 µL
      1 mg 1 x 1 mg 1 x 2 mg 1 x 1 mL

      1 Using the maximum amount of antibody may result in less labelling per antibody. 

      2 Ideal antibody concentration is 1 mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

       

      Buffer Requirements for Conjugation

      Buffer should be pH 6.5-8.5.

      Compatible buffer constituents 
      If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

      50mM / 0.6% Tris1 0.1% BSA2 50% glycerol
      0.1% sodium azide3 PBS Potassium phosphate
      Sodium chloride HEPES Sucrose
      Sodium citrate EDTA Trehalose

      1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
      2 BSA can also interfere with the use of the conjugated antibody in tissue staining.

      Incompatible buffer constituents

      Thiomerosal Proclin Glycine
      Arginine Glutathione DTT

      If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

      Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

      Storing and handling conjugation kits

      Lyophilized Lightning-Link® components are hygroscopic.

      Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 mg 100 µg 3 x 10 µg 3 x 100 µg
      ab274010 - Dylight&reg; 405 Conjugation Mix 1 x 1mg 1 x 100µg 3 x 10µg 3 x 100µg
      ab273994 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl
      ab273995 - Quencher reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Conjugation Kits
      • Dylight

    Associated products

    • Related Products

      • Antibody Concentration And Clean-Up Kit (ab102778)
      • Antibody Purification Kit (Protein A) (ab102784)

    Images

    • conjugation
      conjugation

    Protocols

    • Protocol Booklet - Chinese Version
    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab201798? Please let us know so that we can cite the reference in this datasheet.

    ab201798 has been referenced in 3 publications.

    • Adam A  et al. Multiplexed FluoroSpot for the Analysis of Dengue Virus- and Zika Virus-Specific and Cross-Reactive Memory B Cells. J Immunol 201:3804-3814 (2018). PubMed: 30413671
    • Rizvi AH  et al. Single-cell topological RNA-seq analysis reveals insights into cellular differentiation and development. Nat Biotechnol 35:551-560 (2017). PubMed: 28459448
    • Katoh H  et al. Immunogenetic Profiling for Gastric Cancers Identifies Sulfated Glycosaminoglycans as Major and Functional B Cell Antigens in Human Malignancies. Cell Rep 20:1073-1087 (2017). PubMed: 28768193

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab201798.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.