DyLight® 405 Conjugation Kit (Fast) - Lightning-Link® (ab201798)

This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link^® Rapid DyLight^® 405 Labeling Kit. 321-0015 is the same as the 1 mg size. 321-0010 is the same as the 3 x 100 ug size. 321-0030 is the same as the 3 x 10 ug size. 321-0005 is the same as the 100 ug size.

Amount and volume of antibody for conjugation to DyLight^® 405

¹ Using the maximum amount of antibody may result in less labelling per antibody. 

² Ideal antibody concentration is 1 mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

Buffer Requirements for Conjugation

Buffer should be pH 6.5-8.5.

Compatible buffer constituents 
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

¹ Tris buffered saline is almost always ≤ 50 mM / 0.6%
² BSA can also interfere with the use of the conjugated antibody in tissue staining.

Incompatible buffer constituents

If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

Storing and handling conjugation kits

Lyophilized Lightning-Link^® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

_{DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.}