DyLight® 680 Conjugation Kit (Fast) - Lightning-Link® (ab201804)
Overview
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Product name
DyLight® 680 Conjugation Kit (Fast) - Lightning-Link® -
Product overview
DyLight® 680 Conjugation Kit / DyLight® 680 Labeling Kit ab201804 uses a simple and quick process for DyLight® 680 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
This product is manufactured by Expedeon, an Abcam company. See the table below to match Abcam kit size against Expedeon product code.
To conjugate an antibody to DyLight® 680 using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The DyLight® 680 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to DyLight® 680.
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
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Notes
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® Rapid DyLight® 680 Labeling Kit. 327-0005 is the same as the 100 µg size. 327-0030 is the same as the 3 x 10 ug size. 327-0010 is the same as the 3 x 100 ug size. 327-0015 is the same as the 1 mg size.
Amount and volume of antibody for conjugation to Dylight® 680
Kit size Recommended
amount of antibody1Maximum
amount of antibodyMaximum antibody
volume23 x 10 µg 3 x 10 µg 3 x 20 µg 3 x 10 µL 100 µg 1 x 100 µg 1 x 200 µg 1 x 100µL 3 x 100 µg 3 x 100 µg 3 x 200 µg 3 x 100 µL 1 mg 1 x 1 mg 1 x 2 mg 1 x 1 mL 1 Using the maximum amount of antibody may result in less labelling per antibody.
2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.50mM / 0.6% Tris1 0.1% BSA2 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose 1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
2 BSA can also interfere with the use of the conjugated antibody in tissue staining.Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link® components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 mg 100 µg 3 x 10 µg 3 x 100 µg ab274028 - Dylight® 680 Conjugation Mix 1 x 1mg 1 x 100µg 3 x 10µg 3 x 100µg ab273994 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl ab273995 - Quencher reagent 1 x 200µl 1 x 200µl 1 x 200µl 1 x 200µl -
Research areas
Associated products
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Related Products
Images
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Flow Cytometry - DyLight<680 Conjugation Kit (Fast) Lightning-Link; (ab201804)Image from Heidepriem, Jasmin, et al. Biotechnol J., 15(9): e2000069. doi: 10.1002/biot.202000069. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
Heidepriem, Jasmin, et al used DyLight® 680 Conjugation Kit (Fast) - Lightning-Link® (ab201804) as part of examining the Ebola virus (EBOV). They used the kit to conjugate DyLight™ 680 to anti-HA-peptide antibody for use in peptide microarray.
Peptide microarray siplaying fluorescence from secondary antibodies as a heat map (top: IgG response, bottom: IgM response). The EBOV surface glycoprotein (GP) (676 AA) was mapped as 662 spots of 15-mer peptides with a lateral shift of one AA. Sera from seven recombinant vesicular stomatitisvirus-Zaire ebolavirus-GP (rVSV-ZEBOV-GP) vaccines (numbered) and one EVD survivor were analyzed on separatemicroarrays. The vaccines are listed with the day of serum collection after vaccination. The de?ned epitopes are named by their origin from vaccines(V, in orange), survivor (S, in red) or both (SV, in orange and red) -
Conjugation - DyLight® 680 Conjugation Kit (ab201804)This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab201804 has been referenced in 4 publications.
- Thompson EA et al. Metabolic programs define dysfunctional immune responses in severe COVID-19 patients. Cell Rep 34:108863 (2021). PubMed: 33691089
- Heidepriem J et al. Epitopes of Naturally Acquired and Vaccine-Induced Anti-Ebola Virus Glycoprotein Antibodies in Single Amino Acid Resolution. Biotechnol J N/A:e2000069 (2020). PubMed: 32463974
- Thompson EA et al. Metabolic programs define dysfunctional immune responses in severe COVID-19 patients. medRxiv N/A:N/A (2020). PubMed: 32935120
- Jaenisch T et al. High-density Peptide Arrays Help to Identify Linear Immunogenic B-cell Epitopes in Individuals Naturally Exposed to Malaria Infection. Mol Cell Proteomics 18:642-656 (2019). PubMed: 30630936