FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)

Rey, Elizabeth G., Julia L. Finkelstein, and David Erickson used FITC Conjugation Kit (Fast) - Lightning-Link^® (ab188285) as part of examining folate concentration in human serum. They used the kit to conjugate FITC to FA-BSA conjugates for use in lateral flow assay.
Schematic of sample processing and lateral flow assay, and human serum results. (a) Combination of serum sample with high-pH buffer. (b) Heating of serum solution. (c) Addition of acidic buffer to lower pH. (d) Application of prepared serum solution to LFA. (e) Application of running buffer. (f) Addition of FITC conjugates to nitrocellulose membrane. Human serum results. (g) Mean T/C ratio versus folate concentration for 24 human serum samples. Error bars shown are standard deviation, n = 3. Dotted line shows four-parameter logistic curve fit. (h,i) Images of fluorescent signal from test strip for low and high folate concentration serum, respectively.

Albus, Alexandra, et al used FITC Conjugation Kit (Fast) - Lightning-Link^® (ab188285) as part of examining Naturally occurring autoantibodies (nAb)-Secreting B1 Cells. They used the kit to conjugate FITC to anti-α-Synuclein antibody for use in flow cytometry.
nAbs-secreting B1 cells are rare and highly specific. (A–F) Gating strategy to sort only CD20+ CD27+ CD43+ CD69− IgG+, and Aβ+ (E) /α-Syn+ (F) B cells. Percentages above each plot indicate the proportion of cells resulting from the previous gate. Arrows indicate single cells in gate R5. CD, cluster of differentiation; FSC, forward scatter; SSC, sideward scatter.

Baranowski, Andreas, et al used FITC Conjugation Kit (Fast) - Lightning-Link^® (ab188285) as part of examining whether the bioactive coating of calcium phosphate cements (CPCs) with bone sialoprotein (BSP) results in increased bone formation. They used the kit to conjugate FITC to bone sialoprotein prior to the coating of a three-dimensional printed CPC scaffolds. After several washing steps, the remaining BSP (green) was visualized via microscope and the BSP coating was thus qualitatively evaluated. 

Robinson, Andrew P., et al used FITC Conjugation Kit (Fast) - Lightning-Link^® (ab188285) as part of characterizing oligodendroglial populations. They used the kit to conjugate FITC to Rat anti-PDGFRalpha antibody, clone APA5, for use in flow cytometry.
SJL/J mice were immunized with PLP139–151 and scored daily for clinical disease. A cohort of SJL/J mice was sacrificed, and spinal cords were analyzed by flow cytometry (n = 5). (A) Cells were distinguished from debris by forward and side scatter then singlet cells were gated. Live cells were gated by dead cell exclusion, and CNS resident cells were identified as CD45− or CD45low. (B) Oligodendroglial cells were defined by double positive staining: A2B5+PDGFRα+ early OPCs, A2B5+NG2+ intermediate OPCs, NG2+O4+ late OPCs, O4+MOG+ pre-myelinating oligodendrocytes, and GALC+MOG+ mature oligodendrocytes.

Do, Danh C., et al used FITC Conjugation Kit (Fast) - Lightning-Link^® (ab188285) as part of examining Bla g 2 uptake by human basophils. They used the kit to conjugate FITC to cockroach allergen Bla g 2 for use in immunocytochemistry.
Human basophils were incubated with FITC conjugated Bla g 2 (FITC‐Bla g 2, green) for overnight at 37°C, washed, and analyzed by fluorescent microscopy. Nucleus was stained with the DAPI (blue).

Lydon et al. used ab188285 FITC Conjugation Kit to label mouse anti-sheep CD34 antibody with FITC.

Data shows a) Representative light-scatter (SSC-A) vs fluorescence (CD34-FITC) plot of CD34 expression on cells from ovine apheresis samples using two-colour flow cytometry. B) IgG control.