Gold Conjugation Kit (20 nm, 20 OD) (ab188215)
Overview
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Product name
Gold Conjugation Kit (20 nm, 20 OD)
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Product overview
Abcam’s Gold Conjugation Kit allows antibodies or proteins to be covalently attached to ultra-stable Gold nanoparticles at very high OD quickly and easily. The nanoparticles in the Abcam’s Gold Conjugation Kit have a protective surface coat that can withstand the most extreme conditions (e.g. 2.5M NaOH at 70°C for > 1 hour). The hands-on time for the Abcam Gold conjugation procedure is around 2 minutes and the conjugate is ready to use within 20 minutes. The researcher simply pipettes the biomolecule into a vial containing the nanoparticles of the Gold Conjugation Kit.
The nanoparticles in this kit are supplied as a freeze-dried mixture. The conjugation reaction is initiated simply by reconstituting the freeze-dried nanoparticles with the antibody, which becomes attached (through lysine residues) to the surface of the nanoparticles.
The resulting covalent conjugates are more stable than those prepared by passive adsorption methods. Moreover, unlike passive methods, the coating process is independent of the isoelectric point of the antibody, avoiding the need for extensive trials at different pH values. All antibodies can be labelled at a single pH.
Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.
Benefits
Easy and rapid conjugation – only 2 minutes hands-on time and 100% recovery of materials
Site-specific labelling – Antigen binding sites of antibodies are free to bind the target molecule
Proprietary surface coating prevents metal-protein interactions, and enables covalent attachment to the Gold – Stable conjugates formed
Fully scalable – Easy transfer from R&D to manufacturing
Uniform spherical shape and narrow size distribution – Consistent high quality and excellent batch-to-batch reproducibility
Buffer requirements:
The biomolecule to be conjugated should ideally be in 10 mM amine-free buffer (e.g. MES, MOPS, HEPES), pH range 6.5 to 8.5. Sugars have no effect on conjugation efficiency. For incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits for Nanoparticles. To learn more about incompatible buffers, please refer to the protocol booklet.
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Notes
This product is manufactured by Expedeon, an Abcam company, and was previously called 20nm InnovaCoat® GOLD. 229-0015 is the same as the 1 x 25 µg size. 229-0010 is the same as the 10 x 2.5 µg size. 229-0005 is the same as the 3 x 2.5 µg size.
The 3 and 10 Test Conjugation Kits are designed to label 12 µl of antibody per vial. The 1 Test Conjugation Kit is designed to label 120 µl of antibody per vial.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 3 x 2.5 µg 10 x 2.5 µg 1 x 25 µg ab273947 - Gold 20nm 3 x 2500ng 10 x 2500ng 1 x 25µg ab273943 - Gold Antibody Diluent 1 x 1ml 1 x 4ml 1 x 4ml Gold Quencher Reagent 1 x 700µl 1 x 700µl 1 x 700µl Gold Reaction Buffer 1 x 750µl 1 x 750µl 1 x 750µl -
Research areas
Associated products
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Related Products
Images
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Lateral flow assay - Gold Conjugation Kit (20 nm, 20 OD) (ab188215)Image from Gwyn et al., J Immunol Methods, 435:27-31; doi: 10.1016/j.jim.2016.05.008. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Gwyn, Sarah, et al used Gold Conjugation Kit (20 nm, 20 OD) (ab188215) as part of examining the detection of antibodies against immunodominant antigen Pgp3 from Chlamydia trachomatis. They used the kit to conjugate Gold to Pgp3 protein for use in lateral flow assay.
LFA test. Shown are results of the LFA run with positive (left) and negative (right) control sera. -
GOLD Conjugation Kit (20nm, 20OD)
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab188215 has been referenced in 10 publications.
- Peruzzotti-Jametti L et al. Neural stem cells traffic functional mitochondria via extracellular vesicles. PLoS Biol 19:e3001166 (2021). PubMed: 33826607
- Foggetti A et al. Spiny and Non-spiny Parvalbumin-Positive Hippocampal Interneurons Show Different Plastic Properties. Cell Rep 27:3725-3732.e5 (2019). PubMed: 31242406
- Depannemaecker D et al. Gold Nanoparticles for X-ray Microtomography of Neurons. ACS Chem Neurosci 10:3404-3408 (2019). PubMed: 31274276
- Jiang Q et al. Paper-Based Microfluidic Device (DON-Chip) for Rapid and Low-Cost Deoxynivalenol Quantification in Food, Feed, and Feed Ingredients. ACS Sens 4:3072-3079 (2019). PubMed: 31713421
- Cabezón I et al. Serial block-face scanning electron microscopy applied to study the trafficking of 8D3-coated gold nanoparticles at the blood-brain barrier. Histochem Cell Biol 148:3-12 (2017). PubMed: 28283744
- Anderson CE et al. Rapid Diagnostic Assay for Intact Influenza Virus Using a High Affinity Hemagglutinin Binding Protein. Anal Chem 89:6608-6615 (2017). PubMed: 28499086
- Ortega Arroyo J et al. Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging. Nat Protoc 11:617-33 (2016). PubMed: 26938114
- Gwyn S et al. Lateral flow-based antibody testing for Chlamydia trachomatis. J Immunol Methods 435:27-31 (2016). PubMed: 27208400
- Cabezón I et al. Trafficking of Gold Nanoparticles Coated with the 8D3 Anti-Transferrin Receptor Antibody at the Mouse Blood-Brain Barrier. Mol Pharm 12:4137-45 (2015). PubMed: 26440359
- Wang HK et al. Cellulose-Based Diagnostic Devices for Diagnosing Serotype-2 Dengue Fever in Human Serum. Adv Healthc Mater : (2013). PubMed: 23843297