GST His-tag ELISA Kit (ab302758)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.476 pg/ml
- Range: 35.156 pg/ml - 2250 pg/ml
- Sample type: Cell culture media, Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
-
Product name
GST His-tag ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 7.8% Inter-assay Sample n Mean SD CV% Supernatant 3 9.7% -
Sample type
Cell culture supernatant, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
3.476 pg/ml -
Range
35.156 pg/ml - 2250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 108 92% - 120% Cell culture media 91 81% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Product overview
GST His-tag SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GST His-tag protein. Quantitate GST His-tag with 3.476 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 10X GST His-tag Capture Antibody 1 x 600µl 10X GST His-tag Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml GST His-tag Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
Images
-
Interpolated concentrations of spiked species GST His-tag in serum, lauryl maltoside buffer, urea buffer, 10F RPMI, and Guanidine buffer.
Recombinant GST His-tag was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are as follows: serum 10%, lauryl maltoside buffer 50%, urea buffer 10%, 10F RPMI 50%, and guanidine buffer 0.1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Example of species GST His-tag standard curve in 1X Cell Extraction Buffer PTR.Example of species GST His-tag standard curve. Background-subtracted data values (mean +/- SD) are graphed.
-
Simple Step Elisa ProtocolSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Benefits of Recombinant Antibodies
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab302758 has not yet been referenced specifically in any publications.