HSP70 High Sensitivity ELISA Kit (ab133061)
Key features and details
- Sensitivity: 90 pg/ml
- Range: 0.2 ng/ml - 12.5 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Human
Overview
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Product name
HSP70 High Sensitivity ELISA Kit
See all HSP70 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Buffer 20 5.11ng/ml 3.9% Buffer 20 2.19ng/ml 11.4% Buffer 20 0.99ng/ml 5.9% Inter-assay Sample n Mean SD CV% Buffer 4.98ng/ml 12.8% Buffer 2.63ng/ml 13.7% Buffer 1.08ng/ml 19.1% -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
90 pg/ml -
Range
0.2 ng/ml - 12.5 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94.2 % - % Plasma 79.3 % - % -
Assay time
4h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
Abcam’s HSP70 High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of HSP70 in samples from. This assay allows for the quantitative determination of inducible HSP70 from cell lysates and tissue extracts.
A HSP70 mouse monoclonal antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A HSP70 polyclonal antibody is then added, incubated and washed. An HRP conjugated anti-IgG antibody is then added, incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
Get higher sensitivity in only 90 minutes with Human HSP70 ELISA Kit (ab187399) from our SimpleStep ELISA® range.
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Notes
HSP70 is a molecular chaperone whose expression is induced upon exposure of the cell or organism to conditions of stress. It prevents protein aggregation and promotes the refolding of proteins that become damaged in response to environmental insults, pathogens, and disease. Its activity is essential for cellular survival and recovery under stress conditions, as well as for the maintenance of normal cellular function under non-stress conditions. HSP70 has been implicated to play a role in a variety of disease and physiological processes such as hyperthermia, hypertension, toxic exposure to chemical agents, hypoxia, ischemia, inflammation, autoimmunity, apoptosis, cancer, organ transplantation, and bacterial and viral infections. HSP70 is also a key regulator of many normal physiological processes including aging, spermatogenesis, menstruation, and physical activity such as exercise. The HSP70 high sensitivity ELISA kit is designed to evaluate and monitor HSP70 in these processes, providing a key research tool to understand the role of HSP70 in physiology and disease.
Cross reactivities:
Compound Cross reactivity HSP70 (Human) 100% HSP70 (rat) 117.6% HSP70B’ (Human) 5.4% HSP70 (salmon) 0.8% DnaK (E.coli) 0.5% Hsc70 (bovine) <0.016% Grp78 (hamster) <0.016% Hsp71 (M. tuberculosis) <0.016% Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 100ml Anti-HSP70 Microplate (12 x 8 wells) 1 unit Assay Buffer 28 1 x 50ml Hsp70 Antibody 1 x 10ml HSP70 Horseradish Peroxidase Conjugate 1 x 10ml Plate Sealer 3 units Recombinant HSP70 Standard 1 x 25µl Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Cellular localization
Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs -
Alternative names
- DnaK type molecular chaperone HSP70 1
- Heat shock 70 kDa protein 1/2
- Heat shock 70 kDa protein 1A/1B
see all -
Database links
- Entrez Gene: 3303 Human
- Entrez Gene: 193740 Mouse
- Entrez Gene: 24472 Rat
- Omim: 140550 Human
- SwissProt: P08107 Human
- SwissProt: P0DMV8 Human
- SwissProt: P0DMV9 Human
- SwissProt: Q61696 Mouse
see all
Images
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Typical Standard Curve
Representative Standard Curve using ab133061.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133061 has been referenced in 1 publication.
- Gholamnezhadjafari R et al. Innate inflammatory gene expression profiling in potential brain-dead donors: detailed investigation of the effect of common corticosteroid therapy. Innate Immun 23:440-448 (2017). PubMed: 28504607