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    products/elisa/human-albumin-elisa-kit-ab179887.pdf

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Cardiovascular Blood Serum Proteins
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RecombinantSimpleStep

Human Albumin ELISA Kit (ab179887)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (8)

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SimpleStep ELISA Protocol Diagram
  • Human Albumin standard curve comparison
  • Standard curve
  • Standard curve
  • Interpolated concentrations of native Albumin in human serum, plasma and cell culture supernatant samples.
  • Serum from eight individual healthy human female donors was measured in duplicate.
  • Linearity of dilution for spiked albumin
  • Linearity of dilution for albumin
  • Sandwich ELISA - Human Albumin ELISA Kit (ab179887)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.1 ng/ml
  • Range: 0.7 ng/ml - 45 ng/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human, Cynomolgus monkey

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Overview

  • Product name

    Human Albumin ELISA Kit
    See all Albumin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 7.6%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.1 ng/ml
  • Range

    0.7 ng/ml - 45 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 104 104% - 105%
    Serum 100 92% - 110%
    Hep Plasma 98 89% - 105%
    EDTA Plasma 104 97% - 109%
    Cit plasma 101 100% - 103%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human, Cynomolgus monkey
  • Product overview

    As of December 12, 2019 Human Albumin ELISA kit has been re-developed with new recombinant monoclonal antibodies to provide improved performance and consistency.


    Human Albumin ELISA kit (ab179887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Albumin protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Albumin with 100 pg/mL sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.


    ASSAY SPECIFICITY


    This kit recognizes native human Albumin protein in serum, plasma, and cell culture supernatant samples only. Urine, saliva, cell and tissue extract samples have not been tested with this kit.


    SPECIES REACTIVITY


    This kit recognizes human Albumin and Cynomolgus monkey albumin protein. If you require a kit that is human-specific, please see ab227933.


    Other species reactivity was determined by measuring 1:4,000,000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.


    Reactivity < 3% was determined for the following species: Mouse, rat, Cow.

  • Notes

    Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Albumin Capture Antibody 1 x 600µl
    10X Human Albumin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Native Human Serum Albumin protein (Lyophilized) (ab205808) 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 2 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Signal transduction proteins ELISA kits
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Processes
  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02768 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • alb
    • ALBU_HUMAN
    • Albumin (32 AA)
    • Albumin (AA 34)
    • cell growth inhibiting protein 42
    • DKFZp779N1935
    • GIG20
    • GIG42
    • Growth inhibiting protein 20
    • growth-inhibiting protein 20
    • OTTHUMP00000220436
    • OTTHUMP00000220438
    • OTTHUMP00000220439
    • PRO0883
    • PRO0903
    • PRO1341
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all
  • Database links

    • Entrez Gene: 213 Human
    • Omim: 103600 Human
    • SwissProt: A2V9Z4 Cynomolgus monkey
    • SwissProt: P02768 Human
    • Unigene: 418167 Human
    • Unigene: 592379 Human

    Associated products

    • Alternative Versions

      • Human Albumin Antibody Pair - BSA and Azide free (ab256595)
    • ELISA kits

      • Human Albumin ELISA Kit (ab108787)
      • Human Albumin ELISA Kit (ab108788)

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Human Albumin standard curve comparison
      Human Albumin standard curve comparison

      Standard Curve comparison between the original human Albumin SimpleStep ELISA kit current human Albumin SimpleStep ELISA kit.

    • Standard curve
      Standard curve

      Background-subtracted data values (mean +/- SD) are graphed.

    • Standard curve
      Standard curve

      Example of human Albumin standard curve in Sample Diluent NS. The Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Albumin in human serum, plasma and cell culture supernatant samples.
      Interpolated concentrations of native Albumin in human serum, plasma and cell culture supernatant samples.

      The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000,000, plasma (EDTA) 1:4,000,000, plasma (citrate) 1:8,000,000, and plasma (heparin) 1:8,000,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 31.5 mg/mL in serum, 75.4 mg/mL in plasma (EDTA), 35.5 mg/mL in plasma (citrate) and 41.3 mg/mL in plasma (heparin).

    • Serum from eight individual healthy human female donors was measured in duplicate.
      Serum from eight individual healthy human female donors was measured in duplicate.

      Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 23.4 mg/mL with a range of 10.5 – 37.1 mg/mL.

    • Linearity of dilution for spiked albumin
      Linearity of dilution for spiked albumin

      Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

      Spiked Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

    • Linearity of dilution for albumin
      Linearity of dilution for albumin

      Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

      Native Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

    • Sandwich ELISA - Human Albumin ELISA Kit (ab179887)
      Sandwich ELISA - Human Albumin ELISA Kit (ab179887)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (8)

    Publishing research using ab179887? Please let us know so that we can cite the reference in this datasheet.

    ab179887 has been referenced in 8 publications.

    • Famulari ES  et al. Human liver stem cells express UGT1A1 and improve phenotype of immunocompromised Crigler Najjar syndrome type I mice. Sci Rep 10:887 (2020). PubMed: 31965023
    • Weisenstein C  et al. Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces. Biomed Opt Express 11:448-460 (2020). PubMed: 32010527
    • Li F  et al. 3D Spheroids with Primary Human Liver Cells and Differential Roles of Kupffer Cells in Drug-Induced Liver Injury. J Pharm Sci N/A:N/A (2020). PubMed: 32145211
    • Kang JA  et al. Ciclopirox inhibits Hepatitis B Virus secretion by blocking capsid assembly. Nat Commun 10:2184 (2019). ELISA ; Human . PubMed: 31097716
    • Wang X  et al. Effect of regional arterial infusion combined with early enteral nutrition on severe acute pancreatitis. J Int Med Res 47:6235-6243 (2019). PubMed: 31662003
    • Smith JW  et al. Aflatoxin and viral hepatitis exposures in Guatemala: Molecular biomarkers reveal a unique profile of risk factors in a region of high liver cancer incidence. PLoS One 12:e0189255 (2017). PubMed: 29236788
    • Dickie AP  et al. The pharmacokinetics and metabolism of lumiracoxib in chimeric humanized and murinized FRG mice. Biochem Pharmacol 135:139-150 (2017). ELISA ; Human . PubMed: 28351678
    • Peng G  et al. Immobilization of native type I collagen on polypropylene fabrics as a substrate for HepG2 cell culture. J Biomater Appl 32:93-103 (2017). ELISA ; Human . PubMed: 28504559

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Review the use of kit for CSF sample

    Average Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    The kit is easy to use. But I found that the variation between the duplicate is big for both standards and samples. I diluted the CSF samples at 3 different concentrations. It showed that 1:12000 dilution worked the best.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 20 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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