Human Apolipoprotein B ELISA Kit (ab190806)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.28 ng/ml
- Range: 18.75 ng/ml - 1200 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Apolipoprotein B ELISA Kit
See all Apolipoprotein B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 8 5.9% Inter-assay Sample n Mean SD CV% Human serum 3 2.4% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5.28 ng/ml -
Range
18.75 ng/ml - 1200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 84 81% - 87% Hep Plasma 114 104% - 120% EDTA Plasma 90 85% - 98% Cit plasma 86 81% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
As of December 5, 2019, Human Apolipoprotein B (APOB) ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human Apolipoprotein B ELISA Kit (ab190806) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apolipoprotein B protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Apolipoprotein B with 5.28 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Apolipoproteins are proteins that bind lipids to form lipoproteins, which carry fats and cholesterol in the bloodstream. Apolipoproteins provide structural support to these lipoproteins, may act as ligands for specific cell surface receptors or may modulate the activity of metabolic enzymes that act on lipoproteins. A major class of apolipoproteins is ApoB, which is found in two forms: ApoB-100 (550 kDa) and ApoB-48 (264 kDa). ApoB-48 is made from the N-terminal 48% of the Apolipoprotein B-100 protein due to a post-transcriptional mRNA editing by the ApoB mRNA editing enzyme ApoBec1. ApoBec1 causes a C to U transition, replacing a CAA glutamine encoding codon for a UAA stop codon and generating ApoB-48. ApoBec1 is expressed in the intestine but not the liver, thus ApoB-48 is secreted by the intestine to become the building block of chylomicrons, while ApoB-100 is secreted by the liver to become a component of very low-density lipoproteins (VLDLs), intermediate-density lipoproteins (IDLs), and low-density lipoproteins (LDLs). Importantly, ApoB is also post-translationally palmitoylated; palmitoylation is necessary for proper assembly of lipoproteins (including proper assembly of the hydrophobic core). Furthermore, ApoB-100 operates on the surface of LDLs to allow binding to the LDL receptor and transport for breakdown of LDLs.
Mutations in the Apolipoprotein B gene can cause several diseases. First, in familial hypobetalipoproteinemia (FHBL), mutations in the Apolipoprotein B gene cause production of abnormally short lengths of ApoB protein, lowering the amount of lipoproteins and hence decreasing the body’s capacity to transport and absorb fat and fat-soluble vitamins. Second, in a form of inherited hypercholesterolemia called familial defective ApoB-100 (FDB), mutations in the Apolipoprotein B gene prevent LDLs from effectively binding to their receptors. As a result, fewer LDLs are removed from the blood, leading to hypercholesterolemia, distribution and buildup of cholesterol into tissues such as the skin and arteries, and an increased risk of coronary artery disease (CAD).
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Apolipoprotein B (ApoB) Capture Antibody 1 x 600µl 10X Human Apolipoprotein B (ApoB) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 5BI 1 x 6ml Denaturant 1 x 500µl Human Apolipoprotein B (ApoB) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similarities
Contains 1 vitellogenin domain. -
Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Alternative names
- Apo B
- Apo B 100
- Apo B-100
see all -
Database links
- Entrez Gene: 338 Human
- Omim: 107730 Human
- SwissProt: P04114 Human
- Unigene: 120759 Human
Associated products
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Alternative Versions
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ELISA kits
Images
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Sandwich ELISA - Human Apolipoprotein B ELISA Kit (ab190806)To learn more about the advantages of SimpleStep ELISA® kits see here.
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Other - Human Apolipoprotein B ELISA Kit (ab190806)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Apolipoprotein B comparisonStandard Curve comparison between the original human MMP3 SimpleStep ELISA kit and current human MMP3 SimpleStep ELISA kit.
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Human Apolipoprotein B standard curve comparisonStandard Curve comparison between human Apolipoprotein B SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human Apolipoprotein B standard curve in Sample Diluent NS + 1X Enhancer.The Apolipoprotein B standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Apolipoprotein B in human serum and plasma samples.The concentrations of Apolipoprotein B were measured in duplicates, interpolated from the Apolipoprotein B standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.5%, plasma (citrate) 0.5%, plasma (EDTA) 0.5%, and plasma (heparin) 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein B concentration was determined to be 181.94 ug/mL in serum, 166.45 ug/mL in plasma (citrate) and 112.11 ug/mL plasma (EDTA), and 249.45 ug/mL plasma (heparin).
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0.5% Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein B concentration was determined to be 217.38 ug/mL with a range of 127.53 – 304.1 ug/mL.
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Sandwich ELISA - Human Apolipoprotein B ELISA Kit (ab190806)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab190806 has been referenced in 9 publications.
- McNamee N et al. A method of separating extracellular vesicles from blood shows potential clinical translation, and reveals extracellular vesicle cargo gremlin-1 as a diagnostic biomarker. Transl Oncol 15:101274 (2022). PubMed: 34800917
- Bushweller L et al. Generation of Human Pluripotent Stem Cell-Derived Polarized Hepatocytes. Curr Protoc 2:e345 (2022). PubMed: 35007406
- Morton RE & Mihna D Apolipoprotein F concentration, activity, and the properties of LDL controlling ApoF activation in hyperlipidemic plasma. J Lipid Res 63:100166 (2022). PubMed: 35016907
- Yang X et al. Exploring the value of Mycobacterium tuberculosis modified lipoprotein as a potential biomarker for TB detection in children. BMC Infect Dis 22:158 (2022). PubMed: 35177035
- Vergauwen G et al. Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions. J Extracell Vesicles 10:e12122 (2021). PubMed: 34429857
- Shearn AIU et al. Analysis of Neat Biofluids Obtained During Cardiac Surgery Using Nanoparticle Tracking Analysis: Methodological Considerations. Front Cell Dev Biol 8:367 (2020). PubMed: 32528952
- Rogers MA et al. Retinoids Repress Human Cardiovascular Cell Calcification With Evidence for Distinct Selective Retinoid Modulator Effects. Arterioscler Thromb Vasc Biol 40:656-669 (2020). PubMed: 31852220
- Walss-Bass C et al. X-Aptamer Technology Identifies C4A and ApoB in Blood as Potential Markers for Schizophrenia. Mol Neuropsychiatry 5:52-59 (2019). PubMed: 31019918
- Gugliucci A et al. Short-term isocaloric fructose restriction lowers apoC-III levels and yields less atherogenic lipoprotein profiles in children with obesity and metabolic syndrome. Atherosclerosis N/A:N/A (2016). Sandwich ELISA ; Human . PubMed: 27451002