Key features and details
- Sensitivity: 0.5 ng/ml
- Range: 0.5 ng/ml - 32 ng/ml
- Sample type: Cell culture extracts, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Bcl-2 ELISA Kit
See all Bcl-2 kits
Intra-assay Sample n Mean SD CV% Overall 8.6% Inter-assay Sample n Mean SD CV% Overall 12%
Sample typeSerum, Plasma, Cell culture extracts
Assay typeSandwich (quantitative)
Range0.5 ng/ml - 32 ng/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Human Bcl-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab119506) is designed for accurate quantitative measurement of Human Bcl-2 concentrations in cell lysates, serum and plasma.
Bcl-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Bcl-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bcl-2 captured on the plate.
Get results in 90 minutes with Human Bcl-2 ELISA Kit (ab202411) from our SimpleStep ELISA® range.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-human Bcl-2 monoclonal antibody 1 x 70µl Human Bcl-2 Standard lyophilized (64 ng/mL upon reconstitution) 2 vials Lysis Buffer (10x) 1 x 15ml Microplate coated with monoclonal antibody to Bcl-2 (12 x 8 wells) 1 unit Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml
FunctionSuppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Tissue specificityExpressed in a variety of tissues.
Involvement in diseaseA chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Sequence similaritiesBelongs to the Bcl-2 family.
DomainBH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
modificationsPhosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
Cellular localizationMitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- Apoptosis regulator Bcl 2
- Apoptosis regulator Bcl-2
- Apoptosis regulator Bcl2
ab119506 has been referenced in 21 publications.
- Saleh N et al. Prodigiosin-Functionalized Probiotic Ghosts as a Bioinspired Combination Against Colorectal Cancer Cells. Probiotics Antimicrob Proteins N/A:N/A (2022). PubMed: 36030493
- Zhou Y et al. MiR-485-3p promotes proliferation of osteoarthritis chondrocytes and inhibits apoptosis via Notch2 and the NF-?B pathway. Immunopharmacol Immunotoxicol 43:370-379 (2021). PubMed: 33961511
- Kuang L & Li L E74-like factor 3 suppresses microRNA-485-5p transcription to trigger growth and metastasis of ovarian cancer cells with the involvement of CLDN4/Wnt/ß-catenin axis. Saudi J Biol Sci 28:4137-4146 (2021). PubMed: 34354393
- Abdel-Rafei MK et al. Influence of Ce3+ Substitution on Antimicrobial and Antibiofilm Properties of ZnCexFe2-xO4 Nanoparticles (X = 0.0, 0.02, 0.04, 0.06, and 0.08) Conjugated with Ebselen and Its Role Subsidised with ?-Radiation in Mitigating Human TNBC and Colorectal Adenocarcinoma Proliferation In Vitro. Int J Mol Sci 22:N/A (2021). PubMed: 34576334
- El-Maksoud AAA et al. Nano Milk Protein-Mucilage Complexes: Characterization and Anticancer Effect. Molecules 26:N/A (2021). PubMed: 34770781