Human Beta-2-Microglobulin ELISA Kit (ab181423)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21.2 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Beta-2-Microglobulin ELISA Kit
See all beta 2 Microglobulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human Serum 8 3.8% Inter-assay Sample n Mean SD CV% Human Serum 3 2% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
21.2 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 91 89% - 94% Urine 94 87% - 100% Serum 91 90% - 92% Hep Plasma 90 88% - 91% EDTA Plasma 90 87% - 89% Cit plasma 90 89% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human B2M ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human Beta-2-Microglobulin ELISA kit (ab181423) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of B2M protein in human serum, plasma, urine and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human B2M with 31.25 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
Notes
Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) involved in the presentation of peptide antigens to the immune system. The full length B2M protein exists as a heterodimer of an alpha chain and a beta chain. Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) where affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation. Persistently high B2M serum levels lead to amyloidosis in patients on long-term hemodialysis.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human B2M Capture Antibody 1 x 600µl 10X Human B2M Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human B2M Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients. -
Cellular localization
Secreted. Detected in serum and urine. - Information by UniProt
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Alternative names
- B2M
- B2MG_HUMAN
- Beta 2 microglobin
see all -
Database links
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
Associated products
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Alternative Versions
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ELISA kits
Images
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Other - Human Beta-2-Microglobulin ELISA Kit (ab181423)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (ab181423)To learn more about the advantages of recombinant antibodies see here. -
Human beta-2-microglobulin standard curve comparisonStandard Curve comparison between the original Human beta-2-microglobulin SimpleStep ELISA kit and current Human beta-2-microglobulin SimpleStep ELISA kit.
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Example of human beta-2 microglobulin standard curve in Sample Diluent NS.The beta-2 microglobulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native beta-2 microglobulin in human serum, plasma, and urine samples.The concentrations of beta-2 microglobulin were measured in duplicates, interpolated from the beta-2 microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:200, plasma (EDTA) 1:400, plasma (heparin) 1:200, and 1:400 urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 1037.2 ng/mL in serum, 657.5 ng/mL in plasma (citrate), 1039.9 ng/mL in plasma (EDTA), 787.5 ng/mL in plasma (heparin), and 1348.2 ng/mL in urine.
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Interpolated concentrations of native beta-2 microglobulin in human HepG2 cell culture supernatant sample.The concentrations of beta-2 microglobulin were measured in duplicates, interpolated from the beta-2 microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 1:32. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 119.4 ng/mL in HepG2 cell culture supernatant.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 1093.9 ng/mL with a range of 727.2 – 1537.0 ng/mL.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab181423 has been referenced in 2 publications.
- Nishikawa A et al. Identification of definitive serum biomarkers associated with disease activity in primary Sjögren's syndrome. Arthritis Res Ther 18:106 (2016). Sandwich ELISA ; Human . PubMed: 27180164
- Shinke H et al. Urinary kidney injury molecule-1 and monocyte chemotactic protein-1 are noninvasive biomarkers of cisplatin-induced nephrotoxicity in lung cancer patients. Cancer Chemother Pharmacol 76:989-96 (2015). Sandwich ELISA ; Human . PubMed: 26407820