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    products/elisa/human-beta-2-microglobulin-elisa-kit-ab181423.pdf

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RecombinantSimpleStep

Human Beta-2-Microglobulin ELISA Kit (ab181423)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Human Beta-2-Microglobulin ELISA Kit (ab181423)
  • Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (ab181423)
  • Human beta-2-microglobulin standard curve comparison
  • Example of human beta-2 microglobulin standard curve in Sample Diluent NS.
  • Interpolated concentrations of native beta-2 microglobulin in human serum, plasma, and urine samples.
  • Interpolated concentrations of native beta-2 microglobulin in human HepG2 cell culture supernatant sample.
  • Serum from ten individual healthy human male donors was measured in duplicate.

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 21.2 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human Beta-2-Microglobulin ELISA Kit
    See all beta 2 Microglobulin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human Serum 8 3.8%
    Inter-assay
    Sample n Mean SD CV%
    Human Serum 3 2%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    21.2 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 91 89% - 94%
    Urine 94 87% - 100%
    Serum 91 90% - 92%
    Hep Plasma 90 88% - 91%
    EDTA Plasma 90 87% - 89%
    Cit plasma 90 89% - 91%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human B2M ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.


    Human Beta-2-Microglobulin ELISA kit (ab181423) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of B2M protein in human serum, plasma, urine and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human B2M with 31.25 pg/mL sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

  • Notes

    Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) involved in the presentation of peptide antigens to the immune system. The full length B2M protein exists as a heterodimer of an alpha chain and a beta chain. Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) where affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation. Persistently high B2M serum levels lead to amyloidosis in patients on long-term hemodialysis.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human B2M Capture Antibody 1 x 600µl
    10X Human B2M Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human B2M Lyophilized Purified Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I
    • Cancer
    • Tumor biomarkers
    • Globulins
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • WBC Antigens
    • HLA
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood cell antigens ELISA kits
  • Function

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in disease

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similarities

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localization

    Secreted. Detected in serum and urine.
  • Target information above from: UniProt accession P61769 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • B2M
    • B2MG_HUMAN
    • Beta 2 microglobin
    • Beta 2 microglobulin
    • Beta 2 microglobulin precursor
    • Beta chain of mhc class 1 proteins
    • Beta chain of MHC class I molecules
    • Beta-2-microglobulin form pI 5.3
    • CDABP0092
    • Hdcma22p
    • IMD43
    see all
  • Database links

    • Entrez Gene: 567 Human
    • Omim: 109700 Human
    • SwissProt: P61769 Human
    • Unigene: 534255 Human
    • Unigene: 702947 Human

    Associated products

    • Alternative Versions

      • Human Beta-2-Microglobulin Antibody Pair - BSA and Azide free (ab241138)
    • ELISA kits

      • Human beta 2 Microglobulin ELISA Kit (ab108885)
      • Human beta 2 Microglobulin ELISA Kit (B2M) (ab99977)

    Images

    • Other - Human Beta-2-Microglobulin ELISA Kit (ab181423)
      Other - Human Beta-2-Microglobulin ELISA Kit (ab181423)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (ab181423)
      Sandwich ELISA - Human Beta-2-Microglobulin ELISA Kit (ab181423)
      To learn more about the advantages of recombinant antibodies see here.
    • Human beta-2-microglobulin standard curve comparison
      Human beta-2-microglobulin standard curve comparison

      Standard Curve comparison between the original Human beta-2-microglobulin SimpleStep ELISA kit and current Human beta-2-microglobulin SimpleStep ELISA kit.

    • Example of human beta-2 microglobulin standard curve in Sample Diluent NS.
      Example of human beta-2 microglobulin standard curve in Sample Diluent NS.

      The beta-2 microglobulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native beta-2 microglobulin in human serum, plasma, and urine samples.
      Interpolated concentrations of native beta-2 microglobulin in human serum, plasma, and urine samples.

      The concentrations of beta-2 microglobulin were measured in duplicates, interpolated from the beta-2 microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:200, plasma (EDTA) 1:400, plasma (heparin) 1:200, and 1:400 urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 1037.2 ng/mL in serum, 657.5 ng/mL in plasma (citrate), 1039.9 ng/mL in plasma (EDTA), 787.5 ng/mL in plasma (heparin), and 1348.2 ng/mL in urine.

    • Interpolated concentrations of native beta-2 microglobulin in human HepG2 cell culture supernatant sample.
      Interpolated concentrations of native beta-2 microglobulin in human HepG2 cell culture supernatant sample.

      The concentrations of beta-2 microglobulin were measured in duplicates, interpolated from the beta-2 microglobulin standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 1:32. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 119.4 ng/mL in HepG2 cell culture supernatant.

    • Serum from ten individual healthy human male donors was measured in duplicate.
      Serum from ten individual healthy human male donors was measured in duplicate.

      Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean beta-2 microglobulin concentration was determined to be 1093.9 ng/mL with a range of 727.2 – 1537.0 ng/mL.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab181423? Please let us know so that we can cite the reference in this datasheet.

    ab181423 has been referenced in 2 publications.

    • Nishikawa A  et al. Identification of definitive serum biomarkers associated with disease activity in primary Sjögren's syndrome. Arthritis Res Ther 18:106 (2016). Sandwich ELISA ; Human . PubMed: 27180164
    • Shinke H  et al. Urinary kidney injury molecule-1 and monocyte chemotactic protein-1 are noninvasive biomarkers of cisplatin-induced nephrotoxicity in lung cancer patients. Cancer Chemother Pharmacol 76:989-96 (2015). Sandwich ELISA ; Human . PubMed: 26407820

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