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    products/elisa/human-cdkn2ap16ink4a-antibody-pair-bsa-and-azide-free-ab244050.pdf

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RecombinantRabMAb

Human CDKN2A/p16INK4a Antibody Pair - BSA and Azide free (ab244050)

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Sandwich ELISA - Human CDKN2A/p16INK4a Antibody Pair - BSA and Azide free (ab244050)
  • Sandwich ELISA - Human CDKN2A Antibody Pair - BSA and Azide free (ab244050)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human CDKN2A/p16INK4a Antibody Pair - BSA and Azide free
    See all CDKN2A/p16INK4a kits
  • Assay type

    ELISA set
  • Range

    2.3 ng/ml - 150 ng/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human CDKN2A/p16INK4a. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab227903), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human CDKN2A Capture Antibody (unconjugated) 1 x 100µg
    Human CDKN2A Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Ink4
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • INK4a/Arf
  • Cellular localization

    Cytoplasmic and Nuclear
  • Alternative names

    • CCM2
    • CDK4 inhibitor p16 INK4
    • CDK4I
    • CDKN2
    • CDKN2A
    • Cell cycle negative regulator beta
    • CMM2
    • Cyclin dependent kinase 4 inhibitor A
    • Cyclin Dependent Kinase Inhibitor 2A
    • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4)
    • Cyclin dependent kinase inhibitor 2A isoform 4
    • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3
    • Cyclin dependent kinase inhibitor p16
    • INK4
    • INK4A
    • MLM
    • MTS1
    • Multiple tumor suppressor 1
    • p14
    • p16
    • P16INK4
    • p16INK4a
    • p19
    • p19Arf
    • TP16
    see all
  • Database links

    • Entrez Gene: 1029 Human
    • Omim: 600160 Human
    • SwissProt: P42771 Human

    Associated products

    • Alternative Versions

      • Human CDKN2A/p16INK4a ELISA Kit (ab227903)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab244050 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA
    Use at an assay dependent concentration.
    Notes
    Sandwich ELISA
    Use at an assay dependent concentration.

    Images

    • Sandwich ELISA - Human CDKN2A/p16INK4a Antibody Pair - BSA and Azide free (ab244050)
      Sandwich ELISA - Human CDKN2A/p16INK4a Antibody Pair - BSA and Azide free (ab244050)
      Representative standard curve from corresponding SimpleStep ELISA® Kit (ab227903), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
    • Sandwich ELISA - Human CDKN2A Antibody Pair - BSA and Azide free (ab244050)
      Sandwich ELISA - Human CDKN2A Antibody Pair - BSA and Azide free (ab244050)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab244050? Please let us know so that we can cite the reference in this datasheet.

    ab244050 has not yet been referenced specifically in any publications.

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