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    products/elisa/human-cxcl1-elisa-kitgro-alpha-ab190805.pdf

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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
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RecombinantSimpleStep

Human CXCL1 ELISA Kit (GRO alpha) (ab190805)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)
  • Human CXCL1 (GRO alpha) Standard Curve Comparison.
  • Human CXCL1 (GRO alpha) Standard Curve Comparison between new and original ELISA kit.
  • Example of human CXCL1 (GRO alpha) standard curve in Sample Diluent NS.
  • Interpolated concentrations of native CXCL1 (GRO alpha) in human serum, plasma and samples.
  • Interpolated concentrations of native CXCL1 (GRO alpha) in human PBMC supernatant from cells treated with 1.5% PHA-M for 46 hours.
  • Specificity of CXCL1 (GRO alpha) signal on unstimulated and stimulated media supernatants.
  • Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.064 pg/ml
  • Range: 2.34 pg/ml - 150 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human CXCL1 ELISA Kit (GRO alpha)
    See all CXCL1/GRO alpha kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 3 1.9%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 8 3.7%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.064 pg/ml
  • Range

    2.34 pg/ml - 150 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95 92% - 97%
    Serum 83 82% - 85%
    Hep Plasma 86 85% - 87%
    Cit plasma 81 80% - 82%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human CXCL1 ELISA Kit (ab190805) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL1 protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL1 with 0.064 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    CXCL1 (GRO alpha) is a member of the CXC family of chemokines. CXCL1 contains the ELR motif and preferentially chemoattracts neutrophils. Human CXCL1 undergoes post-translational modification into three isoforms called CXCL1(4-73), CXCL1(5-73) and CXCL1(6-73). These isoforms are produced by proteolytic cleavage after secretion from peripheral blood monocytes, and all show higher activity in-vitro than the full-length protein. Two additional CXC proteins, CXCL2 (or GRO beta) and CXCL3 (or GRO gamma), share 90% and 86% amino acid sequence homology, respectively, with CXCL1. Human CXCL1 mRNA is expressed in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, CXCL1 mRNA has been detected in mammary fibroblasts and epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. CXCL1 is a growth-regulated gene and is overexpressed constitutively in tumorigenic cells. As a result, elevated levels of CXCL1 can be seen in several types of tumors and cancers, including lung cancer and melanoma.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human CXCL1 Capture Antibody 1 x 600µl
    10X Human CXCL1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human CXCL1 Lyophilized Recombinant Protein (ab9806) 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Immunology
    • Innate Immunity
    • Cytokines
    • Other
    • Kits/ Lysates/ Other
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    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits
    • Neuroscience
    • Processes
  • Function

    Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P09341 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • C-X-C motif chemokine 1
    • chemokine (C-X-C motif) ligand 1
    • Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
    • CINC-1
    • CXCL1
    • Cytokine-induced neutrophil chemoattractant 1
    • Fibroblast secretory protein
    • Fsp
    • Gro
    • Gro 1
    • Gro A
    • GRO protein, alpha
    • GRO-alpha(1-73)
    • GRO-alpha(6-73)
    • Gro1
    • Gro1 oncogene
    • GRO1 oncogene (melanoma growth stimulating activity, alpha)
    • GRO1 oncogene (melanoma growth-stimulating activity)
    • GROa
    • GROA_HUMAN
    • Growth-regulated alpha protein
    • KC
    • KC chemokine, mouse, homolog of
    • Melanoma growth stimulatory activity
    • melanoma growth stimulatory activity alpha
    • Melanoma growth stimulatory activity, alpha
    • MGSA
    • MGSA alpha
    • MGSA-a
    • N51
    • NAP-3
    • NAP3
    • Neutrophil-activating protein 3
    • Platelet-derived growth factor-inducible protein KC
    • Scyb 1
    • Scyb1
    • Secretory protein N51
    • Small inducible cytokine subfamily B, member 1
    see all
  • Database links

    • Entrez Gene: 2919 Human
    • Omim: 155730 Human
    • SwissProt: P09341 Human
    • Unigene: 708652 Human
    • Unigene: 789 Human

    Associated products

    • Alternative Versions

      • Human CXCL1 Antibody Pair - BSA and Azide free (GRO alpha) (ab243979)
    • ELISA kits

      • Human CXCL1 ELISA Kit (GRO alpha) (ab100530)

    Images

    • Other - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)
      Other - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Human CXCL1 (GRO alpha) Standard Curve Comparison.
      Human CXCL1 (GRO alpha) Standard Curve Comparison.

      Standard Curve comparison between human CXCL1 SimpleStep ELISA kit (GRO alpha) and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

    • Human CXCL1 (GRO alpha) Standard Curve Comparison between new and original ELISA kit.
      Human CXCL1 (GRO alpha) Standard Curve Comparison between new and original ELISA kit.

      Standard Curve comparison between new human CXCL1 SimpleStep ELISA kit (GRO alpha) and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.

    • Example of human CXCL1 (GRO alpha) standard curve in Sample Diluent NS.
      Example of human CXCL1 (GRO alpha) standard curve in Sample Diluent NS.

      The CXCL1 (GRO alpha) standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

       

    • Interpolated concentrations of native CXCL1 (GRO alpha) in human serum, plasma and samples.
      Interpolated concentrations of native CXCL1 (GRO alpha) in human serum, plasma and samples.

      The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, platelet poor plasma (citrate) 100%, and platelet poor plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 20.65 pg/mL in serum, 11.10 pg/mL in platelet poor plasma (citrate), and 29.39 pg/mL in platelet poor plasma (Heparin).

       

    • Interpolated concentrations of native CXCL1 (GRO alpha) in human PBMC supernatant from cells treated with 1.5% PHA-M for 46 hours.
      Interpolated concentrations of native CXCL1 (GRO alpha) in human PBMC supernatant from cells treated with 1.5% PHA-M for 46 hours.

      The concentrations of CXCL1 (GRO alpha) were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 17.53 ng/mL in treated PBMC supernatant.

    • Specificity of CXCL1 (GRO alpha) signal on unstimulated and stimulated media supernatants.
      Specificity of CXCL1 (GRO alpha) signal on unstimulated and stimulated media supernatants.

      Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine. Cells were cultured for 2 days at 37˚C in the presence or absence of 1.5% PHA-M. The concentrations of CXCL1 (GRO alpha) were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution. The mean CXCL1 concentration was determined to be 5.11 pg/mL in unstimulated PBMC supernatants, and 17533.21 pg/mL in stimulated PBMC supernatants.

       

    • Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)
      Sandwich ELISA - Human CXCL1 ELISA Kit (GRO alpha) (ab190805)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab190805? Please let us know so that we can cite the reference in this datasheet.

    ab190805 has been referenced in 6 publications.

    • Yang IH  et al. Oxidative stress enhanced the transforming growth factor-ß2-induced epithelial-mesenchymal transition through chemokine ligand 1 on ARPE-19 cell. Sci Rep 10:4000 (2020). PubMed: 32132577
    • Sasaki T  et al. IL-8 induces transdifferentiation of mature hepatocytes toward the cholangiocyte phenotype. FEBS Open Bio 9:2105-2116 (2019). PubMed: 31651102
    • Bardi GT  et al. Detection of Inflammation-Related Melanoma Small Extracellular Vesicle (sEV) mRNA Content Using Primary Melanocyte sEVs as a Reference. Int J Mol Sci 20:N/A (2019). PubMed: 30870978
    • Pu Y  et al. Adiponectin promotes human jaw bone marrow mesenchymal stem cell chemotaxis via CXCL1 and CXCL8. J Cell Mol Med 21:1411-1419 (2017). PubMed: 28176455
    • Suh KJ  et al. EGFR or HER2 inhibition modulates the tumor microenvironment by suppression of PD-L1 and cytokines release. Oncotarget 8:63901-63910 (2017). ELISA . PubMed: 28969039
    • Hashimoto O  et al. Collaboration of cancer-associated fibroblasts and tumour-associated macrophages for neuroblastoma development. J Pathol N/A:N/A (2016). Sandwich ELISA ; Human . PubMed: 27425378

    Customer reviews and Q&As

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    Abreview for ab190805 - Human CXCL1 ELISA Kit (GRO alpha)

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    We measured the concentration of human plasma CXCL1 by using this ab190805 - Human CXCL1 ELISA Kit (GRO alpha). Blood were collected in the vacutainer EDTA tubes, the upper layer of plasma was collected after centrifugation and frozen until determination. Samples were further diluted at 1:4 into Sample Diluent assay according to the protocol, the final optical density from all of samples were between the lowest and the highest standard.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 28 2022

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