For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/elisa/human-emmprin-elisa-kit-ab219631.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Cell Type Markers CD Endothelial Cells
Share by email
Validated using a knockout cell lineRecombinantSimpleStep

Human EMMPRIN ELISA Kit (ab219631)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Other - Human EMMPRIN ELISA Kit (ab219631)
  • Example of human EMMPRIN standard curve in Sample Diluent 50BS.
  • Example of human EMMPRIN standard curve in Sample Diluent NS.
  • Interpolated concentrations of spiked EMMPRIN in human serum and plasma samples.
  • Interpolated concentrations of native EMMPRIN in human serum and plasma samples.
  • Interpolated concentrations of native EMMPRIN in human urine and spiked EMMPRIN in human saliva and PBMC stimulated cultured media.
  • Interpolated concentrations of native EMMPRIN in human saliva and PBMC stimulated cultured media.
  • Interpolated concentrations of native EMMPRIN in human cell culture supernatant samples.
  • Serum from ten individual healthy human male donors was measured in duplicate.
  • Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
  • Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.88 pg/ml
  • Range: 31.3 pg/ml - 200 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

You may also be interested in

Primary
Product image
Anti-IL-18 antibody [EPR19954] (ab207324)
ELISA
Product image
Human IL-1 beta ELISA Kit (ab214025)
Protein
Product image
Recombinant Human CD147 protein (Fc Chimera His Tag) (ab229371)

View more associated products

Overview

  • Product name

    Human EMMPRIN ELISA Kit
    See all CD147 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Urine 8 2.5%
    Inter-assay
    Sample n Mean SD CV%
    Urine 3 3%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.88 pg/ml
  • Range

    31.3 pg/ml - 200 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 112 111% - 113%
    Saliva 95 94% - 95%
    Urine 98 97% - 98%
    Serum 97 91% - 102%
    Cell culture media 103 99% - 110%
    Hep Plasma 115 113% - 117%
    EDTA Plasma 108 107% - 109%
    Cit plasma 102 100% - 105%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Cow
  • Product overview

    Human EMMPRIN ELISA Kit (ab219631) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EMMPRIN protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EMMPRIN with 3.88 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Extracellular matrix metalloproteinase inducer (EMMPRIN), also known as Basigin (BSG), and CD147 is a 363-amino acid protein encoded by the gene BSG. EMMPRIN is a member of the immunoglobin superfamily, and targets the monocarboxylate transporters SLC16A1, SLC16A3, and SLC16A8 to the plasma membrane. EMMPRIN is the determinant for the Oκ blood group system, and has been demonstrated to be a receptor on red blood cells for the malaria parasite, Plasmodium falciparum. In addition to its metalloproteinase-inducing ability, EMMPRIN is thought to regulate several other functions, including spermatogenesis, embryo implantation, neural network formation, and tumor progression. EMMPRIN also has several ligands including cyclophilin proteins, caveolin-1, and integrins.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human EMMPRIN Capture Antibody 1 x 600µl
    10X Human EMMPRIN Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human EMMPRIN Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Endothelial Cells
    • Neuroscience
    • Sensory System
    • Visual system
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
    • Neuroscience
    • Processes
  • Function

    Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
  • Tissue specificity

    Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P35613 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 5A11 antigen
    • 5F7
    • BASI_HUMAN
    • Basigin
    • Basigin (Ok blood group)
    • Blood brain barrier HT7 antigen
    • Bsg
    • CD 147
    • CD147
    • CD147 antigen
    • Collagenase stimulatory factor
    • EMMPRIN
    • Extracellular matrix metalloproteinase inducer
    • Leukocyte activation antigen M6
    • M 6
    • M6
    • M6 leukocyte activation antigen
    • Neurothelin
    • OK
    • OK blood group
    • OK blood group antigen
    • TCSF
    • Tumor cell derived collagenase stimulatory factor
    • Tumor cell-derived collagenase stimulatory factor
    see all
  • Database links

    • Entrez Gene: 682 Human
    • Omim: 109480 Human
    • SwissProt: P35613 Human
    • Unigene: 501293 Human

    Associated products

    • Alternative Versions

      • Human EMMPRIN Antibody Pair - BSA and Azide free (ab241924)

    Images

    • Other - Human EMMPRIN ELISA Kit (ab219631)
      Other - Human EMMPRIN ELISA Kit (ab219631)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human EMMPRIN standard curve in Sample Diluent 50BS.
      Example of human EMMPRIN standard curve in Sample Diluent 50BS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of human EMMPRIN standard curve in Sample Diluent NS.
      Example of human EMMPRIN standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spiked EMMPRIN in human serum and plasma samples.
      Interpolated concentrations of spiked EMMPRIN in human serum and plasma samples.

      The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 1,572 pg/mL in neat serum, 1,440 pg/mL in neat plasma (citrate), 1,516 pg/mL in neat plasma (EDTA), and 1,740 pg/mL in neat plasma (heparin).

    • Interpolated concentrations of native EMMPRIN in human serum and plasma samples.
      Interpolated concentrations of native EMMPRIN in human serum and plasma samples.

      The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 634 pg/mL in neat serum, 531 pg/mL in neat plasma (citrate), 563 pg/mL in neat plasma (EDTA), and 599 pg/mL in neat plasma (heparin).

    • Interpolated concentrations of native EMMPRIN in human urine and spiked EMMPRIN in human saliva and PBMC stimulated cultured media.
      Interpolated concentrations of native EMMPRIN in human urine and spiked EMMPRIN in human saliva and PBMC stimulated cultured media.

      The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%, saliva 100%, and PBMC stimulated culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 3,245 pg/mL in neat urine, 1,135 pg/mL in neat saliva, and 1,111 pg/mL in neat PBMC stimulated culture media.

    • Interpolated concentrations of native EMMPRIN in human saliva and PBMC stimulated cultured media.
      Interpolated concentrations of native EMMPRIN in human saliva and PBMC stimulated cultured media.

      The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 100% and PBMC stimulated culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 69 pg/mL in neat saliva, and 75 pg/mL in neat PBMC stimulated culture media.

    • Interpolated concentrations of native EMMPRIN in human cell culture supernatant samples.
      Interpolated concentrations of native EMMPRIN in human cell culture supernatant samples.

      The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMC media 100%, stimulated PBMC media 100%, untreated MCF-7 media 100%, and treated MCF-7 media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 72 pg/mL in neat stimulated PBMC media and 64 pg/mL in neat treated MCF-7 media. The unstimulated PBMC media and untreated MCF-7 media samples measured below the 7th point of the standard curve. PBMC media samples were cultured in RPMI media with 10% fetal bovine serum (unstimulated) for 24 hours and then stimulated for 48 hours with 1.5% PHAM. MCF-7 media samples were cultured in DMEM media with 10% fetal bovine serum (untreated) and then treated with 0.12 mM NEAA with 1 mM sodium pyruvate (treated) for 24 hours.

    • Serum from ten individual healthy human male donors was measured in duplicate.
      Serum from ten individual healthy human male donors was measured in duplicate.

      Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 625 pg/mL with a range of 445- 836 pg/mL.

    • Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
      Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
      Human CD147 concentration was interpolated from the EMMPRIN (CD147) standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human EMMPRIN ELISA kit (ab219631). Wild-type and CD147 knockout A549 cells (ab273748) were assessed in duplicate (n=2); Jurkat and Raji cells were used as positive and negative controls respectively (n=1). Where samples were run in duplicate, data are represented as the mean and error bars represent standard deviation.
    • Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
      Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab219631? Please let us know so that we can cite the reference in this datasheet.

    ab219631 has been referenced in 1 publication.

    • Thakur A  et al. Label-free sensing of exosomal MCT1 and CD147 for tracking metabolic reprogramming and malignant progression in glioma. Sci Adv 6:eaaz6119 (2020). PubMed: 32637597

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab219631.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.