Human ENO1 ELISA Kit (Alpha-Enolase) (ab181417)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.5 U/ml
- Range: 27.85 U/ml - 800 U/ml
- Sample type: Cell culture extracts, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ENO1 ELISA Kit (Alpha-Enolase) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HeLa extract 8 6.5% Inter-assay Sample n Mean SD CV% HeLa extract 3 11.4% -
Sample type
Serum, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
6.5 U/ml -
Range
27.85 U/ml - 800 U/ml -
Recovery
90.1 %
Sample specific recovery Sample type Average % Range Serum 101 100% - 103% Cell culture extracts 93 92% - 93% Tissue Extracts 104 99% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human ENO1 ELISA kit (ab181417) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ENO1 protein in human serum, cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human ENO1 with 6.5 U/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
Notes
Alpha enolase (ENO1, P06733) ENO1 is localized to cytoplasm. It can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. MBP1, a shorter isoform of the ENO1, binds to the myc promoter and acts as a transcriptional repressor. It may be a tumor suppressor. It locates mainly in nucleus. ENO1 is used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also it is a marker for endometriosis. Antibodies against ENO1 are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. ENO1 is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ENO1 Capture Antibody 1 x 600µl 10X Human ENO1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human ENO1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Cytoplasm. Cell membrane. Cytoplasm › myofibril › sarcomere › M line. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line. -
Alternative names
- 2-phospho-D-glycerate hydro-lyase
- alpha enolase like 1
- alpha-enolase
see all -
Database links
- Entrez Gene: 2023 Human
- Omim: 172430 Human
- SwissProt: P06733 Human
Associated products
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Positive Controls
Images
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Other - Human ENO1 ELISA Kit (Alpha-Enolase) (ab181417)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ENO1 standard curve in 1X Cell Extraction Buffer PTR. -
Interpolated concentrations of native ENO1 in human HeLa cell extract based on a 50 µg/mL extract load and human liver tissue extract based on a 286 µg/mL extract load. -
Human recombinant ENO1 protein (600 ng/mL) and neuron-specific human recombinant ENO2 protein (600 ng/mL) were analyzed with this kit. -
Extracts of human liver and human heart were analyzed with this kit.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab181417 has not yet been referenced specifically in any publications.