Human Exodus 2 ELISA Kit (CCL21) (ab193759)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.8 pg/ml
- Range: 9.4 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Exodus 2 ELISA Kit (CCL21) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 3.4% Inter-assay Sample n Mean SD CV% Overall 3 5.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.8 pg/ml -
Range
9.4 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 104 102% - 106% Cell culture media 99 94% - 105% Hep Plasma 102 99% - 106% Cit plasma 99 93% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Exodus 2 ELISA Kit (CCL21) (ab193759) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Exodus 2 (CCL21) protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Exodus 2 (CCL21) with 3.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Exodus 2 (CCL 21) is a small cytokine in the CC chemokine family, and is involved in inhibition of hemopoiesis and stimulation of chemotaxis. It interacts with atypical chemokine receptor ACKR4 and recruits beta-arrestin to ACKR4.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Exodus 2 Capture Antibody 1 x 600µl 10X Human Exodus 2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Exodus 2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. -
Tissue specificity
Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 6Ckine
- Beta chemokine exodus 2
- Beta-chemokine exodus-2
see all -
Database links
- Entrez Gene: 6366 Human
- Omim: 602737 Human
- SwissProt: O00585 Human
- Unigene: 57907 Human
Images
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Other - Human Exodus 2 ELISA Kit (CCL21) (ab193759)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Exodus 2 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of Human serum within the working range of the assay.Background-subtracted data values (mean +/- SD, n = 3) are graphed
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Observed Exodus 2 levels in pooled donor (n=50), mixed gender normal Human plasma and serum.Mean Human Exodus 2 values fall within expected normal reference ranges (WHO).
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Observed Exodus 2 in individual donor normal Human serum (n=10).Mean Human Exodus 2 is 479 pg/mL and values fall within expected normal reference ranges (WHO).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab193759 has been referenced in 2 publications.
- Suh KJ et al. EGFR or HER2 inhibition modulates the tumor microenvironment by suppression of PD-L1 and cytokines release. Oncotarget 8:63901-63910 (2017). ELISA . PubMed: 28969039
- Yamashita M et al. Lymphangiogenic factors are associated with the severity of hypersensitivity pneumonitis. BMJ Open Respir Res 2:e000085 (2015). ELISA ; Human . PubMed: 26448865