Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15.884 pg/ml
- Range: 97.656 pg/ml - 6250 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Factor IX/PTC ELISA Kit, Fluorescent
See all Factor IX/PTC kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 7.4% Inter-assay Sample n Mean SD CV% Serum 3 10.1% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15.884 pg/ml -
Range
97.656 pg/ml - 6250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105 92% - 113% Hep Plasma 112 103% - 118% EDTA Plasma 106 95% - 115% Cit plasma 116 111% - 119% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Factor IX/PTC, Fluorescent SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor IX/PTC, Fluorescent protein in human serum and plasma. Quantitate Human Factor IX/PTC, Fluorescent with 15.884 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 10X Human Factor IX/PTC, Fluorescent Capture Antibody 1 x 600µl 10X Human Factor IX/PTC, Fluorescent Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Factor IX/PTC, Fluorescent Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. -
Tissue specificity
Synthesized primarily in the liver and secreted in plasma. -
Involvement in disease
Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Domain
Calcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain. -
Post-translational
modificationsActivated by factor XIa, which excises the activation peptide.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Christmas Disease
- Christmas factor
- Coagulant factor IX
see all -
Database links
- Entrez Gene: 2158 Human
- Omim: 300746 Human
- SwissProt: P00740 Human
- Unigene: 522798 Human
Images
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Example of human Factor IX/PTC standard curve in Sample Diluent NS.
Example of human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human Factor IX/PTC in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 0.15%, plasma (citrate) 0.075%, plasma (EDTA) 0.075%, and plasma (heparin) 0.15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of Factor IX/PTC in normal human serum donors.Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor IX/PTC concentration was determined to be 4,867.18 ng/mL with a range of 1,062.16 – 8,981.25 ng/mL.
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Human Factor IX/PTC standard curve comparison.Standard curve comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300325).
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Human Factor IX/PTC serum and plasma comparison.Serum and plasma comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300325).
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Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325) -
Human Factor IX/PTC ELISA Kit, Fluorescent (ab300325)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab300325 has not yet been referenced specifically in any publications.