Human Fibrinogen gamma chain ELISA Kit - Intracellular (ab309309)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.8 pg/ml
- Range: 43.75 pg/ml - 2800 pg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Fibrinogen gamma chain ELISA Kit - Intracellular -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3.5% Inter-assay Sample n Mean SD CV% Extract 3 5.8% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
8.8 pg/ml -
Range
43.75 pg/ml - 2800 pg/ml -
Recovery
98 %
Sample specific recovery Sample type Average % Range Tissue Extracts 102 100% - 104% Cell Lysate 98 92% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Fibrinogen gamma chain - Intracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibrinogen gamma chain - Intracellular protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Fibrinogen gamma chain - Intracellular with 8.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Fibrinogen gamma chain - Intracellular Capture Antibody 1 x 600µl 10X Human Fibrinogen gamma chain - Intracellular Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human Fibrinogen gamma chain - Intracellular Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Alternative names
- B cell growth factor 1
- B cell IgG differentiation factor
- B Cell Stimulatory Factor 1
see all -
Database links
- Entrez Gene: 2243 Human
- Entrez Gene: 2244 Human
- Entrez Gene: 2266 Human
- Entrez Gene: 3565 Human
- Omim: 134820 Human
- Omim: 134830 Human
- Omim: 134850 Human
- Omim: 147780 Human
see all
Images
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Example of Human Fibrinogen gamma chain standard curve in 1X Cell Extraction Buffer PTR
Example of Human Fibrinogen gamma chain standard curve. Background-subtracted data values (mean +/- SD) are graphed
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Interpolated concentrations of Human Fibrinogen gamma chain in HepG2 cell extract and human liver tissue extract.Interpolated concentration of native Fibrinogen gamma chain was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HepG2 Cell Extract 15 µg/mL, and Human Liver Tissue Extract 500 ng/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1x Cell Extraction Buffer PTR
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Human Fibrinogen gamma chain ELISA Kit - Intracellular (ab309309) -
Human Fibrinogen gamma chain ELISA Kit - Intracellular (ab309309)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Sandwich ELISA - Human Fibrinogen gamma chain ELISA Kit - Intracellular (ab309309)To learn more about the advantages of SimpleStep ELISA® kits see here.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab309309 has not yet been referenced specifically in any publications.