Human Fractalkine ELISA Kit (CX3Cl1) (ab192145)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.18 pg/ml
- Range: 3.91 pg/ml - 250 pg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Fractalkine ELISA Kit (CX3Cl1)
See all CX3CL1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human milk 5 3% Inter-assay Sample n Mean SD CV% Human milk 3 4.7% -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
1.18 pg/ml -
Range
3.91 pg/ml - 250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 95% - 98% Milk 86 75% - 97% Urine 93 92% - 95% Serum 94 88% - 98% Hep Plasma 84 83% - 86% EDTA Plasma 88 84% - 91% Cit plasma 97 90% - 102% Cerebral Spinal Fluid 102 95% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Fractalkine ELISA Kit (CX3Cl1) (ab192145) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fractalkine (CX3Cl1) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fractalkine (CX3Cl1) with 1.18 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Fractalkine (CX3CL1 or Neurotactin) is a chemokine produced from a type I transmembrane protein on the cell surface. The soluble chemokine is generated by proteolytic cleavage and represents residues 25-100 of the full protein sequence. Fractalkine expression is primarily on epithelial cells. Fractalkine functions both in the full length transmembrane form (for adhesion) and in the released soluble form (chemoattraction).
The standard protein in this assay is the soluble chemokine portion of Fractalkine. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Fractalkine Capture Antibody 1 x 600µl 10X Human Fractalkine Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Fractalkine Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion. -
Alternative names
- A 152E5.2
- AB030188
- ABCD 3
see all -
Database links
- Entrez Gene: 6376 Human
- Omim: 601880 Human
- SwissProt: P78423 Human
Associated products
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ELISA kits
Images
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Other - Human Fractalkine ELISA Kit (CX3Cl1) (ab192145)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Fractalkine standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Fractalkine in human serum, plasma samples.The concentrations of Fractalkine were measured in duplicates, interpolated from the Fractalkine standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 12.5%, plasma (heparin) 25% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fractalkine concentration was determined to be 107 pg/mL in serum, 67 pg/mL in plasma (citrate), 86 pg/mL in plasma (heparin) and 100 pg/mL in plasma (EDTA).
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Interpolated concentrations of native Fractalkine in human urine, milk and CSF samples.The concentrations of Fractalkine were measured in duplicates, interpolated from the Fractalkine standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 25%, milk 12.5, and CSF 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fractalkine concentration was determined to be 71 pg/mL in urine, 127 pg/mL in milk, and 13 pg/mL in CSF.
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Interpolated concentrations of spike Fractalkine in human cell culture media sample.The concentrations of Fractalkine were measured in duplicates, interpolated from the Fractalkine standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of Fractalkine in Human serum from 10 donors.Serum from 10 apparently healthy male donors was measured in duplicate. The mean Fractalkine concentration was determined to be 83 pg/mL with a range of 50-118 pg/mL.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab192145 has been referenced in 1 publication.
- Abu El-Asrar AM et al. Evaluation of Proteoforms of the Transmembrane Chemokines CXCL16 and CX3CL1, Their Receptors, and Their Processing Metalloproteinases ADAM10 and ADAM17 in Proliferative Diabetic Retinopathy. Front Immunol 11:601639 (2020). PubMed: 33552057