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    products/elisa/human-glutathione-peroxidase-1-elisa-kit-ab193767.pdf

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SimpleStep

Human Glutathione Peroxidase 1 ELISA Kit (ab193767)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (3)

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Other - Human Glutathione Peroxidase 1 ELISA Kit (ab193767)
  • Example of Glutathione Peroxidase 1 standard curve.
  • Titration of Human Heart Homogenate (HHH), Human Liver Homogenate (HLH), HepG2, and HeLa extracts within the working range of the assay.

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.11 ng/ml
  • Range: 0.4 ng/ml - 25 ng/ml
  • Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human Glutathione Peroxidase 1 ELISA Kit
    See all Glutathione Peroxidase 1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4 4.17%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 9.8%
  • Sample type

    Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.11 ng/ml
  • Range

    0.4 ng/ml - 25 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 92% - 101%
    Hep Plasma 99 99% - 100%
    EDTA Plasma 93 91% - 98%
    Cit plasma 93 91% - 97%
    Extraction Buffer 96 91% - 100%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Glutathione Peroxidase 1 ELISA Kit (ab193767) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glutathione Peroxidase 1 protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Glutathione Peroxidase 1 with 0.11 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    GPX1 is a selenium-containing enzyme part of the glutathione system responsible for protecting against oxidative damage by catalyzing the reduction of hydroperoxides with glutathione. The homotetramer, GPX1, utilizes its selenocysteine-containing active site to primarily scavenge hydrogen peroxide. The dimerization of glutathione ultimately provides the electrons to reduce the active site, restoring GPX1’s activity. As a critical component of the regulation of oxidative species within cells, GPX1 is directly involved in metabolism, various cancers, and oxidative stress. This assay is specific to isoform 1 of glutathione peroxidase.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human GPX1 Capture Antibody 1 x 600µl
    10X Human GPX1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human GPX1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 75BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
  • Function

    Protects the hemoglobin in erythrocytes from oxidative breakdown.
  • Sequence similarities

    Belongs to the glutathione peroxidase family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P07203 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • AL033363
    • Cellular glutathione peroxidase
    • Glutathione peroxidase
    • Glutathione peroxidase 1
    • GPx 1
    • GPx-1
    • GPX1
    • GPX1_HUMAN
    • GPXD
    • GSHPx-1
    • GSHPX1
    • MGC14399
    • MGC88245
    see all
  • Database links

    • Entrez Gene: 2876 Human
    • Omim: 138320 Human
    • SwissProt: P07203 Human
    • Unigene: 76686 Human

    Images

    • Other - Human Glutathione Peroxidase 1 ELISA Kit (ab193767)
      Other - Human Glutathione Peroxidase 1 ELISA Kit (ab193767)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of Glutathione Peroxidase 1 standard curve.
      Example of Glutathione Peroxidase 1 standard curve.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Titration of Human Heart Homogenate (HHH), Human Liver Homogenate (HLH), HepG2, and HeLa extracts within the working range of the assay.
      Titration of Human Heart Homogenate (HHH), Human Liver Homogenate (HLH), HepG2, and HeLa extracts within the working range of the assay.

      Background-subtracted data values (mean +/- SD, n = 2) are graphed.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab193767? Please let us know so that we can cite the reference in this datasheet.

    ab193767 has been referenced in 3 publications.

    • Lu M  et al. MiR-134-5p targeting XIAP modulates oxidative stress and apoptosis in cardiomyocytes under hypoxia/reperfusion-induced injury. IUBMB Life 72:2154-2166 (2020). PubMed: 32797709
    • Koziol K  et al. Changes in ?H2AX and H4K16ac levels are involved in the biochemical response to a competitive soccer match in adolescent players. Sci Rep 10:14481 (2020). PubMed: 32879387
    • Togha M  et al. An investigation of oxidant/antioxidant balance in patients with migraine: a case-control study. BMC Neurol 19:323 (2019). PubMed: 31837702

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    We would like to know at what temperature should we store the reconstituted GPX1 human protein standard.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 09 2017

    Answer

    We recommend to store reconstituted standards at -80°C, all other assay components at 4°C, and to keep TMB substrate solution protected from light.

    Read More

    Abcam Scientific Support

    Answered on Nov 09 2017

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