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Human hCG ELISA Kit (ab100533)

Submit a review Q&A (6)References (14)
Typical standard curve
  • Typical standard curve

Key features and details

  • Sensitivity: 50 pg/ml
  • Range: 54.87 pg/ml - 40000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human hCG ELISA Kit

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 50 pg/ml

  • Range

    54.87 pg/ml - 40000 pg/ml

  • Recovery

    > 90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 130 120% - 135%
    Serum 113.7 103% - 124%
    Plasma 90.57 83% - 103%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s hCG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human hCG in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human hCG coated on a 96-well plate. Standards and samples are pipetted into the wells and hCG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human hCG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of hCG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    700X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human hCG 2 vials
    hCG Microplate (12 x 8 wells) 1 unit
    Human hCG Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Relevance

    Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.

  • Alternative names

    • Anterior pituitary glycoprotein hormones common subunit alpha
    • CG ALPHA
    • CG beta
    • CG beta a
    • CGA
    • CGB
    • CGB1
    • CGB2
    • CGB3
    • CGB5
    • CGB7
    • CGB8
    • Choriogonadotropin alpha chain
    • Choriogonadotropin subunit beta
    • Chorionic gonadotrophin alpha subunit
    • Chorionic gonadotrophin chain beta
    • Chorionic gonadotropin alpha polypeptide
    • Chorionic gonadotropin beta 5 subunit
    • Chorionic gonadotropin beta 7 subunit
    • Chorionic gonadotropin beta 8 subunit
    • Chorionic gonadotropin beta chain
    • Chorionic gonadotropin beta polypeptide
    • Chorionic gonadotropin beta polypeptide 1
    • Chorionic gonadotropin beta polypeptide 2
    • Chorionic gonadotropin beta polypeptide 5
    • Chorionic gonadotropin beta polypeptide 7
    • Chorionic gonadotropin beta subunit
    • Follicle stimulating hormone alpha chain
    • Follitropin alpha chain
    • FSH alpha
    • FSHA
    • Glycoprotein hormones alpha chain precursor
    • Glycoprotein hormones alpha polypeptide
    • GPHa
    • GPHA 1
    • GPHA1
    • hCGB
    • Human Chorionic Gonadotropin
    • LHA
    • LSH alpha
    • Luteinizing hormone alpha chain
    • Lutropin alpha chain
    • Thyroid stimulating hormone alpha chain
    • Thyrotropin alpha chain
    • TSH alpha
    • TSHA
    see all

  • Database links

    Images

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100533

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100533

    References (14)

    Publishing research using ab100533? Please let us know so that we can cite the reference in this datasheet.

    ab100533 has been referenced in 14 publications.

    • Cui K  et al. Establishment of Trophoblast-Like Tissue Model from Human Pluripotent Stem Cells in Three-Dimensional Culture System. Adv Sci (Weinh) 9:e2100031 (2022). PubMed: 34813178
    • Io S  et al. Capturing human trophoblast development with naive pluripotent stem cells in vitro. Cell Stem Cell 28:1023-1039.e13 (2021). PubMed: 33831365
    • Sato M  et al. Three-dimensional human placenta-like bud synthesized from induced pluripotent stem cells. Sci Rep 11:14167 (2021). PubMed: 34239021
    • Skjærvø Ø  et al. All-in-one paper-based sampling chip for targeted protein analysis. Anal Chim Acta 1089:56-65 (2019). PubMed: 31627819
    • Tseng AM  et al. Maternal circulating miRNAs that predict infant FASD outcomes influence placental maturation. Life Sci Alliance 2:N/A (2019). PubMed: 30833415
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Question

    wir möchten den hCG Human ELISA Kit (ab100533) in unserem Labor zu Forschungszwecken benutzen und benötigen dafür folgende Informationen:

    - Spezifität: Welche Formen von hCG kann man mit dem ELISA detektieren (also nicked, nonnicked hCG, free beta)?

    - Gegen welche Epitope richten sich die Antikörper (anti-hCG dimer, anti-free beta, Anti-beta C-terminal, anti-common beta1, …)?

    - Eventuell Informationen über die verwendeten Antikörper.

    Read More
    Answer

    Vielen Dank für Ihre Anfrage.

    Ich habe um mehr Informationen aus dem Labor gebeten und freue mich Ihnen folgendes bestätigen zu können:

    1.) Dieses Kit ist getestet intaktes humanes hCG zu erkennen.

    2.) Beide Antikörper sind monoklonale Antikörper.

    Der "Capture" Antikörper reagiert hauptsächlich mit der alpha Untereinheit von hCG und kreuzreagiert auch mit hLH, hTSH und hFSH. Es wurde keinen Kreuzreaktivität mit der freien hCG beta-Untereinheit festgestellt.

    Der "Detection" Antikörper erkennt spezifisch die beta Untereinheit von hCG und kreuzreagiert nicht mit hLH, hTSH or hFSH.

    3.) ab100533 kostet €490 plus €45.00 Versand nach Deutschland.

    Ich hoffe, diese Information ist hilfreich und wünsche viel Erfolg bei Ihrer Forschung.

    Read More

    Question

    Many thanks for your prompt reply. I would also like to ask about the hCG ELISA kit, catalog no: ab100533 if the antibody is polyclonal or monoclonal (it is not mentioned in any of the Datasheet, SDS or Protocol Booklet)?

    Regards

    Read More
    Answer

    Thank you for your enquiry.
    I can confirm that both the capture and detection antibodies used in ab100533 hCG Human ELISA Kit are monoclonal.
    I hope this will be helpful. Please do not hesitate to contact me if you have any further questions.

    Read More

    Question

    I am interested in purchasing your hCG Human ELISA kit (ab100533).  I was also interested in purchasing the antibody/capture ligand that the plates are coated with in order for us to perform an internal control.  I was wondering if you could tell me what specific product that corresponds to. 

    Read More
    Answer

    Unfortunately the components of this kit are considered proprioetary by the lab and as such I do not have the information which you are requesting. However we do offer hCG antibody pairs which are tested and guaranteed in sELISA. The best recommended pairs for sandwich immunoassay (coating - conjugate) are: ab1961 - ab36473 or ab1964 - ab36473

    Read More

    Question

    Hi - further to a question regarding beta-hCG - this kit would not work to analyse samples containing only beta-hCG? Thanks

    Read More
    Answer

    Thank you for contacting us.

    The kit ab100533 is intenteded to detect hCG (intact). If you're looking just to detect hCG beta, you may be interested in ab108638.

    https://www.abcam.com/chorionic-gonadotropin-beta-human-elisa-kit-ab108638.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    1. Can we purchase the above kit in Lisbon or must we order directly from you?           2. Regarding sensitivity is mentioned that is < 50 pg/mL. While checking the  Protocol Booklet I realised that   the standard range vary from 54.87 pg/mL - 40000 pg/mL, being my first reading 0 (assay diluent). As I will get samples at lower range can I perform a sandard curve with lower concentrations?        3. Concerning the units, is mentioned that the results are in pg/mL. As we are an accredited laboratory I must report my results in mIU/mL for hCG. Is there any way to find out the converging rate from pg/mL to mIU/mL?       4. I also would like to reconfirm that kit ab100533 detects only "INTACT HCG" and can be used to detect HCG in urine. In these secific case must the sample be centrifuged prior to analisis?    

    Read More
    Answer

    1. You can purchase this kit directly from us, Abcam is based in Cambridge, England and this will ship directly from that office to you. 2. You do not have to use the same standard dilution scheme in the manual if you do not want.  However, the sensitivity is constant so any concentration measured below 50pg/ml should be viewed skeptically as it may not be real but just background noise. 3. As you may know, IU's measure the biological activity, not mass or weight so in many cases conversion is not possible. However, for some substances the equivalent mass of one IU is later established, and the IU is then officially abandoned for that substance. In this case, based on the spec sheet for the standard used in this ELISA kit, it has an activity of 14,100 U/mg so with this information you should be able to convert pg/ml to mIU/ml. 4. This kit does detect only the intact HCG.

    Read More

    Question

    Dear, I have a question regarding Abcam HCG detection assay (ELISA, ab100533). What types of HCG hormone does this ELISA assay detect? Only whole HCG molecule (intact hormone) and not free beta-subunit? Do the used antibodies detect both subunits or only bind to beta? I would like to clarify this issue as it is crucial for my experiments to know which kind of HCG types the assay is detecting. Waiting to hear from you. Best regards,

    Read More
    Answer

    Thank you for your enquiry. I can confirm that the hCG Human ELISA Kit detects the intact hCG molecule (heterodimer). The capture antibody is a mouse monoclonal and recognizes the alpha subunit (hCG-a). The detection antibody is also a mouse monoclonal and recognizes the beta subunit (hCG-b). I hope this is helpful to you. Should you have any further questions, please do not hesitate to contact us.

    Read More

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