Human hCG ELISA Kit (ab100533)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 54.87 pg/ml - 40000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human hCG ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 50 pg/ml -
Range
54.87 pg/ml - 40000 pg/ml -
Recovery
> 90 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 130 120% - 135% Serum 113.7 103% - 124% Plasma 90.57 83% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s hCG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human hCG in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human hCG coated on a 96-well plate. Standards and samples are pipetted into the wells and hCG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human hCG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of hCG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human hCG 2 vials hCG Microplate (12 x 8 wells) 1 unit Human hCG Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma. -
Alternative names
- Anterior pituitary glycoprotein hormones common subunit alpha
- CG ALPHA
- CG beta
see all -
Database links
- SwissProt: P01233 Human
- SwissProt: P01215 Human
Images
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab100533 has been referenced in 14 publications.
- Cui K et al. Establishment of Trophoblast-Like Tissue Model from Human Pluripotent Stem Cells in Three-Dimensional Culture System. Adv Sci (Weinh) 9:e2100031 (2022). PubMed: 34813178
- Io S et al. Capturing human trophoblast development with naive pluripotent stem cells in vitro. Cell Stem Cell 28:1023-1039.e13 (2021). PubMed: 33831365
- Sato M et al. Three-dimensional human placenta-like bud synthesized from induced pluripotent stem cells. Sci Rep 11:14167 (2021). PubMed: 34239021
- Skjærvø Ø et al. All-in-one paper-based sampling chip for targeted protein analysis. Anal Chim Acta 1089:56-65 (2019). PubMed: 31627819
- Tseng AM et al. Maternal circulating miRNAs that predict infant FASD outcomes influence placental maturation. Life Sci Alliance 2:N/A (2019). PubMed: 30833415