Human Heme Oxygenase 1 ELISA Kit (ab207621)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.9 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Heme Oxygenase 1 ELISA Kit
See all HO 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.1% Inter-assay Sample n Mean SD CV% Serum 3 3.8% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.9 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 83 76% - 88% Serum 87 79% - 94% Cell culture extracts 83 76% - 88% EDTA Plasma 85 73% - 95% Cit plasma 85 77% - 93% Tissue Homogenate 90 86% - 92% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Goat, Cow, Pig -
Product overview
Human Heme Oxygenase 1 ELISA Kit (ab207621) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Heme Oxygenase 1 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Heme Oxygenase 1 with 7.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human Heme Oxygenase 1 protein in serum, plasma, cell culture supernatant, and cell and tissue extract samples.
SPECIES REACTIVITY
This kit recognizes human Heme Oxygenase 1 protein.
Other species reactivity was determined by measuring 50% (dilution) serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution. Reactivity < 3% was determined for the following species: Mouse , Rat , Hamster, Guinea Pig , Dog, Goat, Pig, Cow -
Notes
Heme oxygenase 1 (HO 1) is an enzyme that functions in heme catabolism. The activity of heme oxygenase 1 is to cleave the heme ring to form biliverdin. There are two isozymes of heme oxygenase that have 56% amino acid sequence identity: inducible heme oxygenase 1 and constitutively expressed heme oxygenase 2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human Heme Oxygenase 1 Capture Antibody (lyophilized) 1 vial Human Heme Oxygenase 1 Detector Antibody (lyophilized) 1 vial Human Heme Oxygenase 1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Relevance
Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. Tissue specificity: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). Disease: Heme oxygenase 1 deficiency Similarity: Belongs to the heme oxygenase family. -
Alternative names
- bK286B10
- Heat shock protein 32 kD
- heme oxygenase (decycling) 1
see all -
Database links
- Entrez Gene: 3162 Human
- Omim: 141250 Human
- SwissProt: P09601 Human
- Unigene: 517581 Human
Associated products
-
Alternative Versions
Images
-
Other - Human Heme Oxygenase 1 ELISA Kit (ab207621)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human Heme Oxygenase 1 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Example of human Heme Oxygenase 1 standard curve in Sample Diluent NS.Example of human Heme Oxygenase 1 standard curve in Sample Diluent NS. The Heme Oxygenase 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Example of human Heme Oxygenase 1 standard curve in 1X Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
-
Example of human Heme Oxygenase 1 standard curve in Sample Diluent 1X Extraction Buffer PTR.Example of human Heme Oxygenase 1 standard curve in Sample Diluent 1X Extraction Buffer PTR. The Heme Oxygenase 1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Heme Oxygenase 1 in human serum and plasma (EDTA) samples.The concentrations of Heme Oxygenase 1 were measured in duplicate, interpolated from the Heme Oxygenase 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 1.37 ng/mL in serum and 2.12 ng/mL in plasma (EDTA).
-
Interpolated concentrations of native Heme Oxygenase 1 in human liver homogenate extract and A549 cell extract based on a 15.6 µg/mL liver homogenate extract and 5 µg/mL A549 cell extract load.The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 3.30 ng/mL in liver homogenate extract and 1.60 ng/mL in A549 cell extract.
-
Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 1,630 pg/mL with a range of 391 – 3,198 pg/mL.
-
Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Heme Oxygenase 1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
-
Linearity of dilution.Native Heme Oxygenase 1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR.
-
Sandwich ELISA - Human Heme Oxygenase 1 ELISA Kit (ab207621)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab207621 has been referenced in 2 publications.
- Kraszewska I et al. Variability in Cardiac miRNA-122 Level Determines Therapeutic Potential of miRNA-Regulated AAV Vectors. Mol Ther Methods Clin Dev 17:1190-1201 (2020). PubMed: 32518806
- Fize?an I et al. Responsiveness assessment of a 3D tetra-culture alveolar model exposed to diesel exhaust particulate matter. Toxicol In Vitro 53:67-79 (2018). PubMed: 30081072