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RecombinantSimpleStep

Human HNF-4-alpha ELISA Kit (ab210581)

Submit a review Submit a question References (1)
Other - Human HNF-4-alpha ELISA Kit (ab210581)
  • Example of human HNF4-alpha standard curve in Sample Diluent PTR.
  • Interpolated concentrations of native HNF4-alpha in human HepG2 extract and based on a 40 µg/mL extract load.
  • Sandwich ELISA - Human HNF-4-alpha ELISA Kit (ab210581)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.3 pg/ml
  • Range: 31 pg/ml - 2000 pg/ml
  • Sample type: Cell culture extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human HNF-4-alpha ELISA Kit
    See all HNF-4-alpha kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell extract 5 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Cell extract 3 8.5%

  • Sample type

    Cell culture extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3.3 pg/ml

  • Range

    31 pg/ml - 2000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 103 101% - 107%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human HNF-4-alpha ELISA Kit (ab210581) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HNF-4-alpha protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HNF-4-alpha with 3.3 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Hepatocyte nuclear factor 4 alpha (HNF4-alpha) is a transcription factor involved in the transcription of genes essential for development of the liver, kidney and intestine.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Human HNF-4-alpha ELISA Kit (ab210581)
    Other - Human HNF-4-alpha ELISA Kit (ab210581)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human HNF4-alpha standard curve in Sample Diluent PTR.
    Example of human HNF4-alpha standard curve in Sample Diluent PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native HNF4-alpha in human HepG2 extract and based on a 40 µg/mL extract load.
    Interpolated concentrations of native HNF4-alpha in human HepG2 extract and based on a 40 µg/mL extract load.

    The concentrations of HNF4a were measured in duplicate and interpolated from the HNF4-alpha standard curve and corrected for sample dilution.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean HNF4-alpha concentration was determined to be 1346 µg/mL in HepG2 extract.

  • Sandwich ELISA - Human HNF-4-alpha ELISA Kit (ab210581)
    Sandwich ELISA - Human HNF-4-alpha ELISA Kit (ab210581)
    To learn more about the advantages of recombinant antibodies see here.

References (1)

Publishing research using ab210581? Please let us know so that we can cite the reference in this datasheet.

ab210581 has been referenced in 1 publication.

  • Bansal A  et al. Integrative Omics Analyses Reveal Epigenetic Memory in Diabetic Renal Cells Regulating Genes Associated With Kidney Dysfunction. Diabetes 69:2490-2502 (2020). PubMed: 32747424

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