Human IgG ELISA Kit (ab195215)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.02 ng/ml
- Range: 0.23 ng/ml - 15 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Tissue Lysate, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IgG ELISA Kit
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.4% Inter-assay Sample n Mean SD CV% Serum 3 14.7% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Lysate, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
0.02 ng/ml -
Range
0.23 ng/ml - 15 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 89 80% - 106% Milk 89 83% - 94% Urine 87 82% - 93% Serum 101 88% - 125% Cell culture media 107 96% - 115% Hep Plasma 100 100% - 100% EDTA Plasma 90.17 87% - 93% Cit plasma 100 98% - 102% Cerebral Spinal Fluid 98 88% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Sheep, Rabbit, Goat, Guinea pig, Cow, Dog -
Product overview
Human IgG ELISA kit (ab195215) is designed for the quantitative measurement of IgG protein in human serum, plasma, milk, urine, saliva, culture media and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IgG with 20 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
CROSS REACTIVITY
Human IgM, human IgA and human IgE were prepared at 10 ng/mL and 250 ng/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng/mL and only 0.4% cross-reactivity at 250 ng/mL of IgA.
INTERFERENCE
Purified human IgG was assayed at 5 ng/mL in the presence and absence of 250 ng/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.
SPECIES REACTIVITY
This kit recognizes human IgG protein.
Other species reactivity was determined by measuring 10 ng/mL of purified IgG from various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration of human IgG assayed at the same concentration.
Reactive species: Rhesus Monkey
Reactivity < 3% was determined for the following species: Mouse, Rat, Rabbit, Dog, Goat, Sheep, Cow, Guinea Pig
CALIBRATION
ab195215 (IgG) - This immunoassy is calibrated against a highly purified human IgG. The NIBSC/WHO unclassified purified human IgG/IgM/IgG preperation 67/086. was evaluatied in this kit.
The dose response curve of the unclassified standard parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep IgG kit to approximate NIBSC International units, use the equation below.
NIBSC 67/086 approximate value (IU/mL) = 0.000017 (1.7e-5 IU) x SimpleStep IgG value (ng/mL) -
Notes
Immunoglubulin G (IgG) is a glycoprotein molecule which belongs to the immunoglobulin family of proteins known as antibodies. Immunoglobulins are the key component of humoral immunity. IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. The heavy chains are linked to each other and to the light chain by disulfide bonds. Each molecule has two antigen binding sites. While the amino-terminal portions that exhibit highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IgG Capture Antibody 1 x 600µl 10X Human IgG Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CP 1 x 6ml Human IgG Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- SwissProt: P01857 Human
- SwissProt: P01859 Human
- SwissProt: P01860 Human
- SwissProt: P01861 Human
Images
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Other - Human IgG ELISA Kit (ab195215)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human IgG standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Raw data values for example of Human IgG standard curve in Sample Diluent NSRaw data values are shown in the table
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Example of Human IgG standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Raw data values for example of Human IgG standard curve in 1X Cell Extraction Buffer PTRRaw data values are shown in the table
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IgG levels in individual healthy donors.Ten individual healthy donors were evaluated for the presence of IgG in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution (1:5x106). The mean level of IgG was found at 12.5 mg/mL with a range of 9 – 18.4mg/mL.
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Comparison of IgG levels in Human milk, urine and saliva.Bodily fluids from 3 different donors were evaluated for the presence of IgG using this assay. Results were interpolated from the standard curve in sample diluent NS and corrected for sample dilution (1:2.5x104). The mean levels in Milk were found at 20.7 µg/mL, in Urine at 0.8 µg/mL and in Saliva at 11.1 µg/mL.
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IgG1-4 isotypes are detected by this kitHuman IgG1, IgG2, IgG3 and IgG4 were tested at 5ng/mL
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Linearity of dilution – native IgG in human serum, plasma (citrate, EDTA and Heparin), and culture mediaNative IgG was measured in the human serum, plasma citrate, plasma EDTA, plasma heparin and culture media in a 2-fold dilution series. Sample dilutions were made in Sample Diluent NS for all samples, purified IgG was spiked into culture media and diluted in a 2-fold dilution series in Sample Diluent NS
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Linearity of dilution – native IgG in human milk, urine, saliva and HLH extractNative IgG was measured in milk, saliva, urine and liver homogenate (HLH) in a 2-fold dilution series. Sample dilutions were made in Sample Diluent NS for all samples except for HLH, which was carried out in 1X cell extraction buffer PTR.
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Human IgG ELISA Kit (ab195215)To learn more about the advantages of SimpleStep ELISA® kits see here.
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab195215 has been referenced in 17 publications.
- Hoschler K et al. SARS Antibody Testing in Children: Development of Oral Fluid Assays for IgG Measurements. Microbiol Spectr 10:e0078621 (2022). PubMed: 34985331
- Stegelmeier AA et al. AAV-Vectored Expression of the Vascular Normalizing Agents 3TSR and Fc3TSR, and the Anti-Angiogenic Bevacizumab Extends Survival in a Murine Model of End-Stage Epithelial Ovarian Carcinoma. Biomedicines 10:N/A (2022). PubMed: 35203573
- Huang RL et al. A Lateral Flow Immunoassay Coupled with a Spectrum-Based Reader for SARS-CoV-2 Neutralizing Antibody Detection. Vaccines (Basel) 10:N/A (2022). PubMed: 35214731
- Darwich A et al. BNT162b2 vaccine induces antibody release in saliva: a possible role for mucosal viral protection? EMBO Mol Med 14:e15326 (2022). PubMed: 35393790
- van Lieshout LP et al. AAV-monoclonal antibody expression protects mice from Ebola virus without impeding the endogenous antibody response to heterologous challenge. Mol Ther Methods Clin Dev 26:505-518 (2022). PubMed: 36092367