Human IL-10 ELISA Kit (Interleukin-10) (ab185986)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.4 pg/ml
- Range: 9.4 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-10 ELISA Kit (Interleukin-10)
See all IL-10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 5 5.2% Inter-assay Sample n Mean SD CV% supernatant 3 3.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.4 pg/ml -
Range
9.4 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 108 105% - 111% Serum 109 108% - 112% Cell culture media 106 102% - 111% Hep Plasma 116 113% - 117% EDTA Plasma 109 103% - 118% Cit plasma 111 105% - 119% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human IL-10 ELISA Kit (Interleukin-10) (ab185986) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-10 (Interleukin-10) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-10 (Interleukin-10) with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin 10 (IL-10) is a cytokine involved in immunoregulation through the promotion of Th2 response and inhibition of Th1 responses. It is produced by cells of hematopoietic origin, keratinocytes, placental cytotrophoblasts, and hepatic stellate cells.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-10 Capture Antibody 1 x 600µl 10X Human IL-10 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IL-10 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NBP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
Database links
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
Associated products
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Alternative Versions
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ELISA kits
Images
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KO data
Human IL-10 concentration was interpolated from the IL-10 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-10 ELISA Kit (Interleukin-10) (ab185986). Wild-type and IL-10 knockout THP-1 (ab273763) vehicle control and PMA (100 ng/mL, 56 h) + LPS (1 µg/mL, 24 h) treated cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation.”
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Other - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IL-10 Standard Curve ComparisonStandard Curve comparison between human IL-10 current (new) SimpleStep ELISA kit and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.
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Example of human IL-10 standard curve in Sample Diluent 50BP + Enhancer.The IL-10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IL-10 standard curve in Sample Diluent NS + Enhancer.The IL-10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-10 in human PBMC cell culture supernatant samples.The concentrations of IL-10 were measured in duplicates, interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 462 pg/ml in stimulated PBMC cell culture supernatant. PBMC cell culture supernatant samples were stimulated for 46 hours with 1.5% PHA. Unstimulated PBMC cell culture supernatant samples were also measured; all values were below the detectable range of the assay.
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Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab185986 has been referenced in 6 publications.
- Jost M et al. CRISPR-based functional genomics in human dendritic cells. Elife 10:N/A (2021). PubMed: 33904395
- Zhao X et al. The Relationship Between Circulating Bone Morphogenetic Protein-4 and Inflammation Cytokines in Patients Undergoing Thoracic Surgery: A Prospective Randomized Study. J Inflamm Res 14:4069-4077 (2021). PubMed: 34456582
- Ji Z et al. Neural stem cells induce M2 polarization of macrophages through the upregulation of interleukin-4. Exp Ther Med 20:148 (2020). PubMed: 33093886
- Zhai W et al. Effect of dexmedetomidine on hemodynamic changes and inflammatory responses in patients undergoing off-pump coronary-artery bypass grafting. Exp Ther Med 20:250 (2020). PubMed: 33178348
- Bamodu OA et al. Astragalus polysaccharides (PG2) Enhances the M1 Polarization of Macrophages, Functional Maturation of Dendritic Cells, and T Cell-Mediated Anticancer Immune Responses in Patients with Lung Cancer. Nutrients 11:N/A (2019). PubMed: 31547048
- Huang C et al. Exosomal MALAT1 derived from oxidized low-density lipoprotein-treated endothelial cells promotes M2 macrophage polarization. Mol Med Rep 18:509-515 (2018). PubMed: 29750307