Human IL-15 ELISA Kit (ab100554)

’ai contacté le laboratoire mais malheureusement comme expliqué par téléphone, nous n’avons pas testé ce kit avec Il-15 attaché au récepteur.

De plus, puisque nous ne connaissons pas l’épitope des anticorps utilisé danse ce kit, il est difficile de savoir si l’interaction Il-15-recepteur affectera les résultats.

I am getting back to you in regards to the problems experienced when using this kit.

We have not yet encountered an issue with our ELISA kits that were caused by not printing the capture antibodies. All of our kits are pre-coated.

It is very unlikely that the signal produced from the samples comes from non-specific binding alone. This would imply the background response from the samples is extremely high, some above 1.5 OD. Because of the wash steps involved in the procedure, these readings are simply too high to be considered only non-specific binding to the plastic wells.

According to my lab colleagues there may have been an issue with the standard preparation, or the standard itself had degraded.

If possible, would it be possible to test the second, fresh vial of standard recombinant protein provided with the tips provided?

I have requested the standard OD values we obtained from testing this lot. I will send them to you as soon as I receive them.

Please let me know if you need further assistance.

Thank you for contacting us.

I appreciate your patience and apologize for keeping you waiting.

As none of the standards gave strong signal responses it could be that the standard recombinant protein has degraded. However, because the results obtained with the R&Ds recombinant human protein were identical, it may suggest an issue with the standard preparation.

In my opinion the pre-coated microplate could not have caused these results, because the samples gave good, strong signals.

I would encourage you to use the second, fresh vial of Standard Recombinant IL-15 keeping in mind the following tips for ensuring strong signals:

When preparing your standards, it is very critical to briefly spin down the vial first. The powder may drop off from the cap when opening it if you do not spin down. Be sure to dissolve the powder thoroughly when reconstituting. After adding Assay Diluent to the vial, we recommend inverting the tube a few times, then flick the tube a few times, and then spin it down; repeat this procedure 3-4 times. This is a technique we find very effective for thoroughly mixing the standard without too much mechanical force.
Do not vortex the standard during reconstitution, as this will destabilize the protein.
Once your standard has been reconstituted, it should be used right away or else frozen for later use.
Keep the standard dilutions on ice while during preparation, but the ELISA procedure should be done at room temperature.
Be sure to discard the working standard dilutions after use – they do not store well.



I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Thank you for contacting us.
I am very sorry to hear this kit failed to give the expected results. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
In order to better understand the problem, I’d like to gather further information. I would really appreciate if you could please clarify the following:
- How was the kit stored? And how was the standard stored after reconstitution?
- You mention this kit was successfully used in the past. Could you please provide the lot number which worked fine previously?
- Which assay diluent was used (A or B) with your standard / samples?
I am happy to investigate this case for you, and the event that the kit sent happened to be faulty, I will replace o reimburse it as stated by the Abpromise guarantee.
I look forward to receiving your reply. Have a nice day.

Thank you for contacting us.

Since the protocol recommends to store the concentrateat 4°C for 5 days, this is what would be best. If the concentrate is still good after 21 days and how good it is, that would need to be tried out and is difficult to predict.

On the other hand if you want to be on the safe sideand your samples are very precious, I would suggest using the second vial of item F instead.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.