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  1. Link

    products/elisa/human-il-6-elisa-kit-ab178013.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Validated using a knockout cell lineRecombinantSimpleStep

Human IL-6 ELISA Kit (ab178013)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (76)

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Other - Human IL-6 ELISA Kit (ab178013)
  • Human IL6 standard curve comparison data
  • Example of human IL6 standard curve
  • Example of raw data value for of human IL-6 standard curve
  • Interpolated concentrations of spiked IL6 in human serum and plasma samples
  • Interpolated concentrations of native IL6 in human peripheral blood monocyte (PBMC) cell culture supernatant samples
  • Linearity of dilution for spiked recombinant IL-6
  • Linearity of dilution for native IL-6
  • Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)
  • Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.6 pg/ml
  • Range: 7.8 pg/ml - 500 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human IL-6 ELISA Kit
    See all IL-6 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell Extract 5 2.1%
    Inter-assay
    Sample n Mean SD CV%
    Cell Extract 3 2.4%
  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.6 pg/ml
  • Range

    7.8 pg/ml - 500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 82 77% - 84%
    Cell culture media 100 97% - 103%
    EDTA Plasma 82 80% - 85%
    Cit plasma 84 81% - 85%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IL-6 ELISA kit (ab178013) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 protein in human serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IL-6 with 1.6 pg/ml sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.


    ASSAY SPECIFICITY This kit recognizes both native and recombinant Human IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.


    CROSS REACTIVITY Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.


    INTERFERENCE Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and tested for interference. No interference with was observed. 18.Species Reactivity This kit recognizes Human IL-6 protein. Recombinant mouse IL-6 was prepared at 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed. Please contact our Technical Support team for more information

  • Notes

    Interleukin 6 (IL6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL6. IL6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests 10 x 96 tests
    10X Human IL-6 Detector Antibody 1 x 600µl 10 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml
    Antibody Diluent 5BI 1 x 6ml 10 x 6ml
    Human IL-6 Capture Antibody (Lyophilized) 1 vial 1 x 10 vials
    Human IL-6 Lyophilized Recombinant Protein (ab9627) 1 x 2 vials 10 x 2 vials
    Plate Seals 1 unit 10 units
    Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 10 units
    Stop Solution 1 x 12ml 1 x 120ml
    TMB Development Solution 1 x 12ml 1 x 120ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Neuroscience
    • Neurology process
    • Metabolism
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Interleukin ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Processes
  • Function

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P05231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all
  • Database links

    • Entrez Gene: 3569 Human
    • Omim: 147620 Human
    • SwissProt: P05231 Human
    • Unigene: 654458 Human

    Associated products

    • Alternative Versions

      • Human IL-6 Antibody Pair - BSA and Azide free (ab243973)
    • ELISA kits

      • Human IL-6 ELISA Kit (ab100572)
      • Human IL-6 ELISA Kit (ab100573)
      • Human IL-6 ELISA Kit (ab46027)
      • Human IL-6 ELISA Kit High Sensitivity (ab46042)

    Images

    • Other - Human IL-6 ELISA Kit (ab178013)
      Other - Human IL-6 ELISA Kit (ab178013)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Human IL6 standard curve comparison data
      Human IL6 standard curve comparison data

      Standard curve comparison between the previous Human IL6 SimpleStep ELISA® kit and the re-developed Human IL6 SimpleStep ELISA® kit.  The re-developed kit shows increased sensitivity.

    • Example of human IL6 standard curve
      Example of human IL6 standard curve

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of raw data value for of human IL-6 standard curve
      Example of raw data value for of human IL-6 standard curve

      Raw data values are shown in the table.

    • Interpolated concentrations of spiked IL6 in human serum and plasma samples
      Interpolated concentrations of spiked IL6 in human serum and plasma samples

      The concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Interpolated concentrations of native IL6 in human peripheral blood monocyte (PBMC) cell culture supernatant samples
      Interpolated concentrations of native IL6 in human peripheral blood monocyte (PBMC) cell culture supernatant samples

      The concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC Cell culture supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL6 concentration was determined to be 60,443 pg/mL in stimulated PBMC cell culture supernatant, and undetectable in unstimulated and media controls.

    • Linearity of dilution for spiked recombinant IL-6
      Linearity of dilution for spiked recombinant IL-6

      Recombinant IL-6 was spiked into biological samples and diluted in a 2-fold dilution series in Sample Diluent NS. NL= Non linear 50% pooled serum and plasma (EDTA, Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

      50% serum from ten individual healthy human male and female donors was measured in duplicate. All values were below the detectable range of the assay.

    • Linearity of dilution for native IL-6
      Linearity of dilution for native IL-6

      Native IL-6 was measured in biological samples in a 2- fold dilution series. Sample dilutions are made in Sample Diluent NS.

    • Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)
      Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)

      Human IL-6 concentration was interpolated from the IL-6 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-6 ELISA kit (ab178013). Wild-type and IL-6 knockout A549 cells (ab273751) were assessed in duplicate (n=2). Cells were either treated with 20 ng/mL active recombinant human IL-1 beta protein (ab259387) for 24 h to induce expression of IL-6 or not treated with IL-1 beta. Data are represented as the mean and error bars represent standard deviation.

    • Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)
      Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (76)

    Publishing research using ab178013? Please let us know so that we can cite the reference in this datasheet.

    ab178013 has been referenced in 76 publications.

    • Wang T  et al. The Protective Effects of Ramelteon Against Isoflurane-Induced Insults and Inflammatory Response in Brain Microvascular Endothelial Cells. Neurotox Res 39:677-686 (2021). PubMed: 33211285
    • Li N  et al. Astragaloside IV alleviates silica-induced pulmonary fibrosis via inactivation of the TGF-ß1/Smad2/3 signaling pathway. Int J Mol Med 47:N/A (2021). PubMed: 33448318
    • Dai X  et al. Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization. Bioact Mater 6:2029-2038 (2021). PubMed: 33474514
    • Shi C  et al. lncRNA SNHG14 Plays a Role in Sepsis-Induced Acute Kidney Injury by Regulating miR-93. Mediators Inflamm 2021:5318369 (2021). PubMed: 33505213
    • Wang H  et al. HDAC1-mediated deacetylation of HIF1a prevents atherosclerosis progression by promoting miR-224-3p-mediated inhibition of FOSL2. Mol Ther Nucleic Acids 23:577-591 (2021). PubMed: 33510945
    View all Publications for this product

    Customer reviews and Q&As

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    The association of NOV/CCN3 with obstructive sleep apnea (OSA): preliminary evidence of a novel biomarker in OSA.

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    Abreviews
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    I tried many different products to get IL-6 better and more clear but failed then I bought ab178013 - Human IL-6 ELISA Kit in serum as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best IL-6 ELISA Kit to detect IL-6 in vivo studies
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 12 2018

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