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    products/elisa/human-il-8-elisa-kit-ab108869.pdf

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Cardiovascular Angiogenesis Cytokines Interleukin
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Human IL-8 ELISA Kit (ab108869)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (1)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.01 ng/ml
    • Range: 0.016 ng/ml - 1 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum, Tissue
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human IL-8 ELISA Kit
      See all IL-8 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 4.1%
      Inter-assay
      Sample n Mean SD CV%
      Overall 7.6%
    • Sample type

      Cell culture supernatant, Serum, Plasma, Tissue
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      > 0.01 ng/ml
    • Range

      0.016 ng/ml - 1 ng/ml
    • Recovery

      97 %

    • Assay time

      5h 00m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s IL-8 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-8 concentrations in Human cell culture supernatants, tissue extracts, serum and plasma.


      An IL-8 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-8 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-8 captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent N Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 2 x 30ml
      50X Biotinylated Human IL-8 Antibody 1 x 120µl
      Chromogen Substrate 1 x 7ml
      IL-8 Microplate (12 x 8 well strips) 1 unit
      IL-8 Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 11ml
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Cytokines
      • Interleukin
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Immunology
      • Innate Immunity
      • Cytokines
      • Other
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Interleukin ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cytokines and cytokine receptors ELISA kits
      • Neuroscience
      • Processes
    • Function

      IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
    • Sequence similarities

      Belongs to the intercrine alpha (chemokine CxC) family.
    • Post-translational
      modifications

      Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P10145 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • (Ala-IL-8)77
      • (Ser-IL-8)72
      • 9E3
      • Beta thromboglobulin like protein
      • C-X-C motif chemokine 8
      • CEF-4
      • chemokine, CXC motif, ligand 8
      • CXCL8
      • Emoctakin
      • GCP-1
      • GCP/IL-8 protein I
      • GCP/IL-8 protein II
      • GCP/IL-8 protein III
      • GCP/IL-8 protein IV
      • GCP/IL-8 protein V
      • GCP/IL-8 protein VI
      • GCP1
      • Granulocyte chemotactic protein 1
      • IL-8
      • IL-8(1-77)
      • IL-8(9-77)
      • IL8
      • IL8/NAP1 form I
      • IL8/NAP1 form II
      • IL8/NAP1 form III
      • IL8/NAP1 form IV
      • IL8/NAP1 form V
      • IL8/NAP1 form VI
      • IL8_HUMAN
      • Inteleukin 8
      • LECT
      • LUCT
      • Lymphocyte-derived neutrophil-activating factor
      • LYNAP
      • MDNCF
      • MDNCF-b
      • MDNCF-c
      • MONAP
      • Monocyte derived neutrophil activating peptide
      • Monocyte derived neutrophil chemotactic factor
      • Monocyte-derived neutrophil chemotactic factor
      • Monocyte-derived neutrophil-activating peptide
      • NAF
      • NAP 1
      • NAP-1
      • NAP1
      • Neutrophil activating peptide 1
      • Neutrophil activating protein 1
      • Neutrophil-activating factor
      • Neutrophil-activating protein 1
      • Protein 3 10C
      • Protein 3-10C
      • SCYB 8
      • SCYB8
      • Small inducible cytokine subfamily B member 8
      • T cell chemotactic factor
      • T-cell chemotactic factor
      • TSG 1
      • TSG1
      see all
    • Database links

      • Entrez Gene: 3576 Human
      • Omim: 146930 Human
      • SwissProt: P10145 Human
      • Unigene: 624 Human

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab108869.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab108869? Please let us know so that we can cite the reference in this datasheet.

      ab108869 has been referenced in 1 publication.

      • Seo Y  et al. Punicalagin Ameliorates Lupus Nephritis via Inhibition of PAR2. Int J Mol Sci 21:N/A (2020). PubMed: 32674502

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Dear Tech Support Team,

      Kindly advise if the following products can be used with human ascites:

      https://www.abcam.com/High-Sensitivity-IL6-Human-ELISA-Kit-1-x-96-Well-Plate-ab46042.html

      https://www.abcam.com/High-Sensitivity-IL6-Human-ELISA-Kit-2-x-96-Well-Plates-ab46044.html

      https://www.abcam.com/IL6-Human-Elipair-Kit-Reagents-for-10-x-96-Well-Plates-ab48479.html

      https://www.abcam.com/IL6-Human-Elipair-Kit-Reagents-for-5-x-96-Well-Plates-ab48478.html

      https://www.abcam.com/IL6-Human-ELISA-Kit-ab108867.html

      https://www.abcam.com/IL6-Human-ELISA-Kit-1-x-96-Well-Plate-ab46027.html

      https://www.abcam.com/IL8-Human-ELISA-Kit-1-x-96-Well-Plate-ab46032.html

      https://www.abcam.com/IL8-Human-ELISA-Kit-ab100575.html

      https://www.abcam.com/IL8-Human-ELISA-Kit-ab108869.html

      https://www.abcam.com/IL8-Human-ELISA-Kit-2-x-96-Well-Plates-ab46049.html

      https://www.abcam.com/IL8-Human-ELISA-Kit-1-x-96-Well-Plate-ab100576.html

      Thanks in advance for your assistance and reply.
      Best Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Feb 27 2012

      Answer

      Thank you for contacting us. I apologise for the delay.
      Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
      The only problem I would suspect is the high concentration of IgG in ascites which can interfere with the ELISA. I would suggest diluting samples 1:2 or 1:5 to minimize the matrix effect.
      I hope this helps and if we can assist further, please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      Answered on Feb 27 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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