Human Insulin ELISA Kit (ab100578)
Key features and details
- Sensitivity: 4 µlU/ml
- Range: 4.69 µlU/ml - 300 µlU/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Insulin ELISA Kit
See all Insulin kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 4 µlU/ml -
Range
4.69 µlU/ml - 300 µlU/ml -
Recovery
89 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 76.16 68% - 88% Serum 91.4 83% - 102% Plasma 99.03 73% - 128% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Product overview
Abcam’s Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Insulin and Proinsulin in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This kit detects both insulin and proinsulin.
Get higher sensitivity in only 90 minutes with Human Insulin ELISA Kit (ab200011) from our SimpleStep ELISA® range.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Insulin 2 vials Insulin Microplate (12 strips x 8 wells) 1 unit Recombinant Human Insulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. -
Involvement in disease
Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IDDM
- IDDM1
- IDDM2
see all -
Database links
- Entrez Gene: 3630 Human
- Entrez Gene: 16333 Mouse
- Entrez Gene: 397415 Pig
- Entrez Gene: 24505 Rat
- Omim: 176730 Human
- SwissProt: P01317 Cow
- SwissProt: P01308 Human
- SwissProt: P01325 Mouse
see all
Images
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Sandwich ELISA - Insulin Human ELISA Kit (ab100578)
Human Insulin measured in cell culture supernatants showing quantity (µIU) per mL of tested sample. Samples diluted 1-2 fold.
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Sandwich ELISA - Insulin Human ELISA Kit (ab100578)Human Insulin measured in biological fluids showing quantity (µIU) per mL of tested sample. Samples diluted 1-6 fold.
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Sandwich ELISA - Insulin Human ELISA Kit (ab100578)Human Insulin standard curve: mean of duplicates (+/- SD), performed in Diluent A
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Typical Standard CurveRepresentative Standard Curve using ab100578.
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Typical Standard CurveRepresentative Standard Curve using ab100578.
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab100578 has been referenced in 39 publications.
- Vega-Torres JD et al. Short-term exposure to an obesogenic diet during adolescence elicits anxiety-related behavior and neuroinflammation: modulatory effects of exogenous neuregulin-1. Transl Psychiatry 12:83 (2022). PubMed: 35220393
- Zhao H et al. Modified Banxia Xiexin Decoction Ameliorates Polycystic Ovarian Syndrome With Insulin Resistance by Regulating Intestinal Microbiota. Front Cell Infect Microbiol 12:854796 (2022). PubMed: 35619648
- Liu R et al. MicroRNA-21 promotes pancreatic β cell function through modulating glucose uptake. Nat Commun 13:3545 (2022). PubMed: 35729232
- Jia X et al. Divergent neurocircuitry dissociates two components of the stress response: glucose mobilization and anxiety-like behavior. Cell Rep 41:111586 (2022). PubMed: 36351404
- Bizjak DA et al. Running for Your Life: Metabolic Effects of a 160.9/230 km Non-Stop Ultramarathon Race on Body Composition, Inflammation, Heart Function, and Nutritional Parameters. Metabolites 12:N/A (2022). PubMed: 36422277