Human Interferon gamma ELISA Kit (ab300323)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.428 pg/ml
- Range: 7.813 pg/ml - 500 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Hep Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Interferon gamma ELISA Kit
See all Interferon gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 7.4% Inter-assay Sample n Mean SD CV% Supernatant 3 11.38% -
Sample type
Cell culture supernatant, Saliva, Serum, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.428 pg/ml -
Range
7.813 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 97% - 115% Serum 101 96% - 104% Hep Plasma 103 93% - 114% EDTA Plasma 100 93% - 106% Cit plasma 101 95% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Interferon gamma SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon gamma protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Interferon gamma with 3.428 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 1 x 384 tests 10X Human Interferon gamma Capture Antibody 1 x 600µl 1 x 600µl 10X Human Interferon gamma Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit Antibody Diluent 4BI 1 x 6ml 1 x 6ml Human Interferon gamma Lyophilized Recombinant Protein 2 vials 2 vials Plate Seals 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 12ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit Stop Solution 1 x 12ml 2 x 12ml TMB Development Solution 1 x 12ml 2 x 12ml -
Research areas
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Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IF 1
- IFG
- IFI
see all -
Database links
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
Images
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Example of human Interferon-gamma standard curve in Sample Diluent NS.
Example of human Interferon-gamma standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human Interferon-gamma in stimulated PBMC cell culture supernatant.Interpolated concentration of native Interferon-gamma was measured in duplicate at different sample concentrations. Undiluted samples are 2.5% stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of Human Interferon-gamma in PBMC cell culture supernatants.Interpolated concentration of native Interferon-gamma was measured in duplicate in human PBMC cell culture supernatants. Sample dilutions are made in Sample Diluent NS. Human PBMCs were cultured for 46 hours in the presence or absence of 1.5% PHA-M. The concentrations of human Interferon gamma were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the Interferon gamma standard curve. The interpolated values are plotted (mean +/- SD, n=2).
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Human Interferon-gamma competitor standard curve comparison.Standard curve comparison between Human Interferon-gamma SimpleStep ELISA kit (ab300323) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.
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Human Interferon gamma ELISA Kit (ab300323) -
Human Interferon gamma ELISA Kit (ab300323)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab300323 has not yet been referenced specifically in any publications.