Human Lactoferrin ELISA Kit (ab200015)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 48.8 pg/ml
- Range: 156.3 pg/ml - 10000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Lactoferrin ELISA Kit
See all Lactoferrin kits
Intra-assay Sample n Mean SD CV% Human Serum 8 5.1% Inter-assay Sample n Mean SD CV% Human Serum 3 5.4%
Sample typeSaliva, Milk, Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range156.3 pg/ml - 10000 pg/ml
Sample specific recovery Sample type Average % Range Saliva 120 113% - 126% Milk 109 99% - 118% Urine 99 99% - 100% Serum 102 100% - 105% Cell culture media 86 86% - 86% Hep Plasma 106 105% - 110% EDTA Plasma 108 105% - 110% Cit plasma 110 109% - 111%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Does not react with: Cow
As of October 11 2019, Human Lactoferrin ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human Lactoferrin ELISA Kit (ab200015) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lactoferrin protein in edta plasma, hep plasma, milk, saliva, serum, urine, cell culture media, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Lactoferrin with 48.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
This kit recognizes native human Lactoferrin protein in serum, plasma, milk, urine, saliva, and cell culture supernatant samples only.
Cell and tissue extract samples have not been tested with this kit.
Recombinant human Transferrin protein was prepared at within the working range of the Lactoferrin standard curve and assayed for cross reactivity. No cross-reactivity was observed.
Recombinant human Transferrin protein was prepared at 50 ng/mL and 2 ng/mL and assayed for interference in the presence 2 ng/mL human Lactoferrin. No Interference was observed.
This kit recognizes human Lactoferrin protein.
Other species reactivity was determined by measuring 0.5% serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow, Monkey
Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactoferrin is a member of the transferrin family and is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. High levels of Lactoferrin are found in saliva and tears, intermediate levels in serum and plasma, and low levels in urine. The canonical sequence of Lactoferrin (known as isoform 1) is 710 amino acids in length and runs at 78.2 kDa. Isoform Delta-Lactoferrin (or DeltaLF) is missing amino acids 1 – 44 of the canonical sequence. Mouse and rat Lactoferrin are 71% and 62% identical to human Lactoferrin, respectively.
Lactoferrin possesses antibacterial, antiviral, anti-inflammatory, and immunoregulatory properties. The presence of Lactoferrin in neutrophils and the release of Lactoferrin during inflammation suggest that Lactoferrin is involved in phagocytic killing and immune responses. Additionally, Lactoferrin mediates bacteriostasis through its ability to sequester free iron and inhibit microbial growth. Lactoferrin also directs bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. Furthermore, Lactoferrin may play a role in the regulation of bone growth because it has anabolic, differentiating, and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis. Salivary Lactoferrin plays an important role in protecting the oral cavity against several oral pathogens including Streptococcus mutans. Additionally, increased levels of Lactoferrin in fecal samples is used as a marker of active inflammatory bowel disease (IBD).
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human Lactoferrin Capture Antibody 1 x 600µl 10X Human Lactoferrin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Lactoferrin Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains.
- Information by UniProt
- Growth inhibiting protein 12
- Entrez Gene: 4057 Human
- Omim: 150210 Human
- SwissProt: P02788 Human
- Unigene: 529517 Human
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The Lactoferrin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Example of human Lactoferrin standard curve in Sample Diluent NS. The Lactoferrin standard curve was prepared. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of Lactoferrin were measured in duplicates, interpolated from the Lactoferrin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:200, plasma (heparin) 1:200, and plasma (EDTA) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lactoferrin concentration was determined to be 1,142 ng/mL in neat serum, 885.1 ng/mL in neat plasma (citrate), 1,054 ng/mL in neat plasma (heparin), and 581.4 ng/mL in neat plasma (EDTA).
The concentrations of Lactoferrin were measured in duplicates, interpolated from the Lactoferrin standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1:500,000, urine 1:20, and saliva 1:10,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lactoferrin concentration was determined to be 4,021.6 µg/mL in neat milk (defatted), 0.033 µg/mL in neat urine, and 39.1 µg/mL in neat saliva.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lactoferrin concentration was determined to be 473.8 ng/mL with a range of 315.2 – 852.7 ng/mL.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Lactoferrin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Native Lactoferrin was measured in human milk, urine, and saliva samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Native purified Lactoferrin protein was spiked into RPMI 1640 cell culture media containing 10% fetal bovine serum and measured in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
ab200015 has been referenced in 6 publications.
- Bartolome F et al. Standardizing salivary lactoferrin measurements to obtain a robust diagnostic biomarker for Alzheimer's disease. Alzheimers Dement (Amst) 13:e12173 (2021). PubMed: 33969170
- Hoshino A et al. Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers. Cell 182:1044-1061.e18 (2020). PubMed: 32795414
- González-Sánchez M et al. Decreased salivary lactoferrin levels are specific to Alzheimer's disease. EBioMedicine 57:102834 (2020). PubMed: 32586758
- Mao Y et al. Processed human amniotic fluid retains its antibacterial activity. J Transl Med 17:68 (2019). PubMed: 30823930
- Patras KA et al. Augmentation of Urinary Lactoferrin Enhances Host Innate Immune Clearance of Uropathogenic Escherichia coli. J Innate Immun 11:481-495 (2019). PubMed: 31055580
- Pérez-Is L et al. The N125S polymorphism in the cathepsin G gene (rs45567233) is associated with susceptibility to osteomyelitis in a Spanish population. PLoS One 14:e0220022 (2019). PubMed: 31647805