Human MCP3 ELISA Kit (CCL7) (ab193769)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10.3 pg/ml
- Range: 46.875 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MCP3 ELISA Kit (CCL7)
See all MCP3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 2% Inter-assay Sample n Mean SD CV% Overall 3 6.8% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10.3 pg/ml -
Range
46.875 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99.7 98.4% - 101.2% Cell culture media 107.3 103.8% - 112.9% Hep Plasma 98.5 97% - 99.5% EDTA Plasma 87.8 84.7% - 89.6% Cit plasma 99 95.6% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human MCP3 ELISA Kit (CCL7) (ab193769) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP3 (CCL7) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MCP3 (CCL7) with 10.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MCP3 (CCL7) is a monomeric secreted chemotactic factor of intercrine (chemokine cc) family. MCP3 attracts monocytes and eosinophils, but not neutrophils. MCP3 augments monocyte anti-tumor activity. It also induces the release of gelatinase B. MCP3 protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MCP3 Capture Antibody 1 x 600µl 10X Human MCP3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human MCP3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 7
- Ccl7
- CCL7_HUMAN
see all -
Database links
- Entrez Gene: 6354 Human
- Omim: 158106 Human
- SwissProt: P80098 Human
- Unigene: 251526 Human
Images
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Other - Human MCP3 ELISA Kit (CCL7) (ab193769)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of MCP3 standard curve prepared in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Comparison of MCP3 concentrations in unstimulated and PHA-stimulated Human PBMC.PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 48 hours. MCP3 concentrations were measured in 4 X and 8 X diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC, and 10F RPMI1640 media. Raw data values (mean +/-SD, n=2) are graphed. The dotted line represents Blank control.
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Example of MCP3 standard curve prepared in Sample Diluent 50 BP.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of 48 hours PHA-stimulated PBMC cell culture supernatant samples within the working range of the assay.Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab193769 has been referenced in 2 publications.
- Kong M et al. Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury. Redox Biol 46:102079 (2021). PubMed: 34454163
- Szilagyi JT et al. Per- and Polyfluoroalkyl Substances Differentially Inhibit Placental Trophoblast Migration and Invasion In Vitro. Toxicol Sci 175:210-219 (2020). PubMed: 32219433