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Human MMP3 ELISA Kit (ab100607)

Reviews (1)Q&A (7)References (4)
Typical Standard Curve

    Key features and details

    • Sensitivity: 0.3 ng/ml
    • Range: 0.41 ng/ml - 100 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human MMP3 ELISA Kit
      See all MMP3 kits

    • Detection method

      Colorimetric

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 0.3 ng/ml

    • Range

      0.41 ng/ml - 100 ng/ml

    • Recovery

      95 %

      Sample specific recovery
      Sample type Average % Range
      Cell culture supernatant 94.72 84% - 103%
      Serum 95.39 83% - 103%
      Plasma 96.25 85% - 104%

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      Abcam’s MMP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP3 pro and active forms in serum, plasma, and cell culture supernatants. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended).


      This assay employs an antibody specific for Human MMP3 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


      Get results in 90 minutes with Human MMP3 ELISA Kit (ab189572) from our SimpleStep ELISA® range.


       

    • Notes

      Optimisation may be required with urine samples.

    • Platform

      Microplate

    Properties

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100607.

    References (4)

    Publishing research using ab100607? Please let us know so that we can cite the reference in this datasheet.

    ab100607 has been referenced in 4 publications.

    • Liu X  et al. Antagonism of NK-1R using aprepitant suppresses inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes. Artif Cells Nanomed Biotechnol 47:1628-1634 (2019). PubMed: 31010320
    • Han HS  et al. Hydrangea serrata (Thunb.) Ser. Extract Attenuate UVB-Induced Photoaging through MAPK/AP-1 Inactivation in Human Skin Fibroblasts and Hairless Mice. Nutrients 11:N/A (2019). PubMed: 30823635
    • Miro C  et al. Thyroid hormone induces progression and invasiveness of squamous cell carcinomas by promoting a ZEB-1/E-cadherin switch. Nat Commun 10:5410 (2019). PubMed: 31776338
    • Qiu WJ  et al. MicroRNA-27a alleviates IL-1ß-induced inflammatory response and articular cartilage degradation via TLR4/NF-?B signaling pathway in articular chondrocytes. Int Immunopharmacol 76:105839 (2019). PubMed: 31520995

    Customer reviews and Q&As

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    1-8 of 8 Abreviews or Q&A

    Abreview for MMP3 Human ELISA Kit

     Good Good 4/5 (Ease of Use)
    Abreviews
    abreview image

    Sample: Human Cell (Skin fibroblasts)
    Specification: Skin fibroblasts
    Amount of Sample: 200 µg
    Type of Kit Used: ELISA
    Positive control: Included in kit as part of standard curve.
    Did you use the Abcam Kit protocol?: Yes

    Additional data
    Additional Notes: Incubation times are a little longer than in some other kits, which must be remembered when planning multiple experiments, but the data generated is of a better quality overall.

    The graph shows the differing levels of MMP3 released by primary fibroblasts exposed to several different active ingredients dissolved in media for several days prior to media collection and analysis.

    (Sorry I cant say what the actives actually are due to business nature of the work).
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 09 2012

    Question


    Thank you for your mail. We guess that some misunderstanding occurred according to ****'s last e-mail (see below), the costumer decided to receive a credit for this 2 units of ab100607. Please issue a credit note.

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    Answer

    Thank you very much for clarifying this so quickly.
    I canceled the order with the free of charge replacement and edited a credit note reference CN ********.
    Sorry for my mistake and thank you for being so quick with your reply.

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    Question

    I discussed once again with the customer and here is the summary. Originally, 2 kits were ordered.

    Read More
    Answer

    Thank you for kindly confirming these details.
    As requested, we arranged a free of charge replacement (2 units of kit ab100607).
    Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

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    Question

    Pleaseaddthereplacementkitab100607toZotal'scurrentorderthatwillbeshippedonFriday.

    Read More
    Answer

    Thank you for your email.

    I am happy to process the replacement. Could you please confirm how many kits (ab100607) your customer ordered originally?

    Thank you.

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    Question

    Please see below customer's answers:

    1.) When has the kit been used and when have the components been reconstituted? How has the kit before and after reconstitution been stored?

    The kit was reconstituted and used on the same day, May 21st 2012 (together with the MMP-1 kit). Both before and after reconstitution they were kept at 4 deg C


    2.) Did you have any troubles reconstituting the standard?


    No. We subsequently tested the standard dilutions by dot blot with another anti-MMP3 antibody an obtained a reasonable signal.

    3.) Have you any comment on which component might be faulty? Have you, for example, tested whether the adding some of the HRP conjugated antibody to an aliquot of the substrate itself would give a signal?


    We have no idea for “ faulty component” but we can mention that the substrate was OK since we used the pooled substrates from kit MMP-3 and MMP-1 for both assays - in MMP1 plate we got a nice standard curve. We have indication that standard was OK since we got reaction with another batch of anti MMP3 antibody in a different method. We did not test the HRP antibody.


    4.) Just for our records, what kind of samples have you used?


    Our samples are skin surface washes of patients suffering from dermatitis and psoriasis (a small vessel with PBS is attached to skin for 30 min). Lesional skin from such patients is known to over-express these proteins. And indeed, in a previous group of patients tested by dot blot (and by using different antibodies) we obtained the expected results.



    Please advise.






    Thanks in advance.

    Read More
    Answer

    Thank you very much for confirming all these details. This is indeed very useful for us.
    It looks like the customer has received a kit with a faulty HRP / HRP conjugate. We will therefore be happy to replace the kit for the customer. Please let us know if the customer would accept a replacement kit or if he would prefer a credit note. I apologize for all the inconvenience caused.
    I look forward hearing back from you.

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    Question

    T ELISA on MMP3 (ab100607) kit, strictly following all the instructions and using all the materials / solutions in proper dilutions as indicated in the kit. The only difference was that incubation with the substrate was prolonged to 45 min, after seeing very weak response in 30 min. However, the signal was still weak with a poor standard curve. The file with original data is attached. For comparison, the customer included the data obtained in the ELISA with the MMP1 kit as well (ab100604), that was run in parallel to the MMP3 test, which gave a nice standard curve. In order to test the system, they performed a dot blot with the same samples (standard + experimental samples) , however with another MMP-3 antibody, which worked nicely. The standard curve and samples were detected, so the protein was there... Please advise.

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    Answer

    rmmarize the problem. I am sorry to hear that this kit is not providing satisfactory results.
    We have not yet received any negative feedback and I am not able yet to tell where the problem might is coming from. I would therefore appreciate if you can confirm some further details:
    1.) When has the kit been used and when have the components been reconstituted? How has the kit before and after reconstitution been stored?
    2.) Did the customer had any troubles reconstituting the standard?
    3.) Has the customer any comment on which component might be faulty? Has he for example tested whether the adding some of the HRP conjugated antibody to an aliquot of the substrat itself would give a signal?
    4.) Just for our records, what kind of samples has the customer used?
    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.
    I hope this information is helpful, and I thank you for your cooperation.

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    Question

    Sorry for this late reply but we would be interested to test the MMP-3 kit (ab100607) in serum samples of monkeys and get our 100 % discount since this kit has only be validated for human samples.

    In addition, we are also interested to measure the IL23 cytokine(ab64708) in serum samples of monkeys. This kit is also only validated for human samples. The range of this kit is 156.2 pg/ml - 5000 pg/ml but the sensitivity is less than 20 pg/ml. So samples can be analyzed up to 20 pg/ml. Do you think it would be possible to get a discount for this kit as well?

    How many sample volume do we need for the MMP-3 kit and for the IL-23 kit? We only have 300ul -400ul of serum sample available.

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    Answer

    Thank you for your interest in the MMP-3 and IL23 kits.

    The volume of sample required for each test is 100ul per well of the assay plate.

    Regarding the 100% discount, I want to be sure you understand the offer, which is also described at the following link:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    The discount is not applied to the purchase of the kits. The kits are purchased full-price after we issue you a code. After you test the kits on the monkey serum, you then send Abcam a review of your results (with the code ID in the review), via our Abreview form. ( Please see www.abcam.com/abreviews).

    After we receive your reviews, the code is then "active" and can be redeemed for a free kit. Or, it can be used to receive a 20% discount on a primary antibody or a protein.

    In addition, 440 points will be awarded for the first review published for ELISA products. Please see https://www.abcam.com/index.html?pageconfig=resource&rid=14713 for more details.

    These offers are available through June.

    Please let me know if you are still interested, and I will send you the codes, which are necessary before you place your order.

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    Question

    I wish to test 400 serum samples using your ELISA kits for: 1. Osteoprotegerin (Catalogue number ab100617) 2. MMP3 (Catalogue number ab100607) 3. DKK1 (Catalogue number ab100501)   I wonder if you could answer a few queries for me: 1. Please could you provide me with a 'Kit Insert' for each kit? - I was unable to find a link on your website. 2. Can I use these specific kits on an automated ELISA machine? - I would prefer not to test 400 samples individually. 3. As I would like to order 6 kits for each biomarker (thus 18 kits in total) would you be able to give me a more competitive rate than that stated onthe website? 4. Some of our serum samples have been stored frozen for 3-5 years. Will this affect the assay?   Would you prefer to discuss during a site visit?   I look forward to hearing from you.  

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    Answer

    Thank you for your interest in our kits. I will send the product inserts first thing tomorrow morning, EST. The kits will be compatible with the automated ELISA machine, if the automated set-up approximates the conditions specified in the kit protocols. We provide a discount for orders of 5 or more kits. I will have our customer service department prepare a proforma. I do not anticipate any issues with the age of the sera, if they have been stored at a consistent temperature of -20C or less. You may want to conduct a trial before committing to a large run, however.

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