Human NOV ELISA Kit (Nephroblastoma Overexpressed) (ab193710)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2 pg/ml - 30000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human NOV ELISA Kit (Nephroblastoma Overexpressed)
See all CCN3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
2 pg/ml - 30000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 109.5 102% - 118% Plasma 99.29 93% - 106% Cell culture media 130.4 118% - 139% -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s NOV (Nephroblastoma Overexpressed) Human ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human NOV in serum, plasma, and cell culture supernatant.
This assay employs an antibody specific for human NOV coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures NOV present in the samples. The wells are washed and biotinylated anti-human NOV antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of NOV bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated Human NOV detection antibody 2 vials Human NOV Standards (lyophilized) 2 vials Pre-coated Human NOV Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Immediate-early protein likely to play a role in cell growth regulation. -
Tissue specificity
Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CCN family member 3
- CCN3
- IBP-9
see all -
Database links
- Entrez Gene: 4856 Human
- Omim: 164958 Human
- SwissProt: P48745 Human
- Unigene: 235935 Human
Images
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Typical standard curve in Assay Diluent A
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Typical standard curve in Assay Diluent BData provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab193710 has been referenced in 1 publication.
- Li H et al. The Predictive Role of Tenascin-C and Cellular Communication Network Factor 3 (CCN3) in Post Hepatectomy Liver Failure in a Rat Model and 50 Patients Following Partial Hepatectomy. Med Sci Monit 25:6755-6766 (2019). PubMed: 31494663