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RecombinantSimpleStep

Human PCSK9 ELISA Kit (ab209884)

Submit a review Q&A (1)References (3)
Other - Human PCSK9 ELISA Kit (ab209884)
  • Human PCSK9 standard curve comparison data.
  • Example of human PCSK9 standard curve in Sample Diluent NS
  • Example of human PCSK9 standard curve in 1X Extraction Buffer PTR
  • . Interpolated concentrations of native PCSK9 in human serum and plasma samples
  • Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extract
  • Serum from six individual human male donors was measured in duplicate
  • Sandwich ELISA - Human PCSK9 ELISA Kit (ab209884)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 68 pg/ml
  • Range: 0.5 ng/ml - 32 ng/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human PCSK9 ELISA Kit
    See all PCSK9 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human serum 5 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 4.6%

  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    68 pg/ml

  • Range

    0.5 ng/ml - 32 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 106 103% - 109%
    Serum 105 104% - 107%
    Cell culture extracts 92 91% - 94%
    Hep Plasma 111 107% - 115%
    EDTA Plasma 96 93% - 100%
    Cit plasma 104 102% - 106%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow, Cynomolgus monkey, Macaque monkey

  • Product overview

    Human PCSK9 ELISA Kit (ab209884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PCSK9 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PCSK9 with 68 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial player in the regulation of plasma cholesterol homeostasis. Degradation of low-density lipid receptor family members (LDLR, VLDLR, APOER, and APOER2) is promoted in intracellular acidic compartments following binding by PCSK9.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Human PCSK9 ELISA Kit (ab209884)
    Other - Human PCSK9 ELISA Kit (ab209884)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Human PCSK9 standard curve comparison data.
    Human PCSK9 standard curve comparison data.

    Standard curve comparison between human PCSK9 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold increase in sensitivity.

  • Example of human PCSK9 standard curve in Sample Diluent NS
    Example of human PCSK9 standard curve in Sample Diluent NS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of human PCSK9 standard curve in 1X Extraction Buffer PTR
    Example of human PCSK9 standard curve in 1X Extraction Buffer PTR

    Background-subtracted data values (mean +/- SD) are graphed.

  • . Interpolated concentrations of native PCSK9 in human serum and plasma samples
    . Interpolated concentrations of native PCSK9 in human serum and plasma samples

    The concentrations of PCSK9 were measured in duplicates, interpolated from the PCSK9 standard curves and corrected for sample dilution. Undiluted samples are as follows:  serum 10%, plasma (citrate) 10%, and plasma (heparin) 10%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean PCSK9 concentration was determined to be 207 ng/mL in serum, 117 ng/mL in plasma (citrate), 166 ng/mL in plasma (EDTA), and 292 ng/mL in plasma (heparin).

  • Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extract
    Interpolated concentrations of native PCSK9 in HepG2 cell culture supernatant and cell extract

    The concentrations of PCSK9 were measured in duplicate, interpolated from the PCSK9 standard curve, and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 50%, HepG2 cell extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean PCSK9 concentration was determined to be 7.3 ng/mL in HepG2 cell culture supernatant and 15 ng/mL in HepG2 cell extract.

     

  • Serum from six individual human male donors was measured in duplicate
    Serum from six individual human male donors was measured in duplicate

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean PCSK9 concentration was determined to be 208 ng/mL with a range of 125 - 317 ng/mL.

  • Sandwich ELISA - Human PCSK9 ELISA Kit (ab209884)
    Sandwich ELISA - Human PCSK9 ELISA Kit (ab209884)
    To learn more about the advantages of recombinant antibodies see here.

References (3)

Publishing research using ab209884? Please let us know so that we can cite the reference in this datasheet.

ab209884 has been referenced in 3 publications.

  • Kongmalai T  et al. The effect of temperature on the stability of PCSK-9 monoclonal antibody: an experimental study. Lipids Health Dis 20:21 (2021). PubMed: 33632254
  • Gan ES  et al. Dengue virus induces PCSK9 expression to alter antiviral responses and disease outcomes. J Clin Invest 130:5223-5234 (2020). PubMed: 32644974
  • Tóth Š  et al. Elevated Circulating PCSK9 Concentrations Predict Subclinical Atherosclerotic Changes in Low Risk Obese and Non-Obese Patients. Cardiol Ther 6:281-289 (2017). PubMed: 28623549

Customer reviews and Q&As

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Question


Inquiry: Does this test measure furin cleaved PCSK9 also?

Read More
Answer

The antibodies were raised against the C terminal fragment, but the epitope has not been mapped. So most likely it will not recognize the furin cleaved N terminal fragment, but will recognize the  C terminal fragment.

Read More

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