Human PDGF B ELISA Kit, Fluorescent (ab300326)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.57 pg/ml
- Range: 1.95 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PDGF B ELISA Kit, Fluorescent
See all PDGF B kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 4.7% Inter-assay Sample n Mean SD CV% Plasma 3 6% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.57 pg/ml -
Range
1.95 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 108 80% - 122% Serum 99 95% - 103% Hep Plasma 105 93% - 116% EDTA Plasma 118 110% - 124% Cit plasma 86 81% - 89% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PDGF B, Fluorescent SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGF B, Fluorescent protein in human serum, plasma and cell culture supernatant. Quantitate Human PDGF B, Fluorescent with 1.57 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits.
Single-wash protocol reduces assay time to 90 minutes or less.
High sensitivity, specificity and reproducibility from superior antibodies.
Fully validated in biological samples.
96-wells plate breakable into 12 x 8 wells strips.
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 10X Human PDGF B, Fluorescent Capture Antibody 1 x 600µl 10X Human PDGF B, Fluorescent Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human PDGF B, Fluorescent Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
- Information by UniProt
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Alternative names
- Becaplermin
- c sis
- FLJ12858
see all -
Database links
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- Unigene: 1976 Human
Images
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Example of human PDGF B standard curve in Sample Diluent NS.
Example of human PDGF B standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human PDGF B in serum, plasma (EDTA), plasma (citrate), and plasma (heparin).Interpolated concentration of native PDGF B was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, plasma (citrate) 25%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of human PDGF B in stimulated PBMC culture supernatant.Interpolated concentration of native PDGF B was measured in duplicate at different sample concentrations. Undiluted samples are 50% stimulated (1.5% PHA-M, 2-days) PBMC cell culture (107 cells/mL) supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of PDGF B in normal human serum donors.Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean PDGF B concentration of detected samples was determined to be 1607.65 pg/mL with a range of undetected – 2896.75 pg/mL.
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PDGFB monomer and homodimer reactivity.Recombinant human PDGF B monomeric form and recombinant human PDGF B dimeric form were measured in duplicates at 250 pg/mL. Interpolated values are plotted (mean +- SD, n=2).
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Human PDGF B ELISA Kit, Fluorescent (ab300326) -
Human PDGF B ELISA Kit, Fluorescent (ab300326)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab300326 has not yet been referenced specifically in any publications.