Human PDGF BB ELISA Kit (ab184860)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.9 pg/ml
- Range: 11.7 pg/ml - 750 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman PDGF BB ELISA Kit
See all PDGF B kits
Intra-assay Sample n Mean SD CV% Human Serum 8 3.3% Inter-assay Sample n Mean SD CV% Human Serum 3 5.3%
Sample typeSerum, Plasma, Cell culture media
Assay typeSandwich (quantitative)
Range11.7 pg/ml - 750 pg/ml
Sample specific recovery Sample type Average % Range Serum 95 85% - 100% Cell culture media 104 101% - 106% Hep Plasma 104 92% - 114% EDTA Plasma 99 91% - 107% Cit plasma 109 100% - 114%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human PDGF BB ELISA kit (ab184860) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGF BB protein in human serum, plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human PDGF BB with 2.9 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
Platelet derived growth factor B chain plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. As a homodimer it is a potent mitogen for cells of mesenchymal origin and is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGF-BB is essential for normal blood vessel development, for normal development of kidney glomeruli and in wound healing. Signaling is modulated by the formation of heterodimers with PDGF A chain to yield PDGF-AB.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human PDGF BB Capture Antibody 1 x 600µl 10X Human PDGF BB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human PDGF BB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
- Information by UniProt
- c sis
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- Unigene: 1976 Human
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The PDGF BB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Background subtracted data from duplicate measurements are plotted.
Mean human PDGF-BB is 145 pg/mL and values fall within expected normal reference ranges (WHO).
Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours aliquoted and assayed for endogenous PDGF-BB levels.
Standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed. In addition, PDGF-A was prepared at 15 ng/mL and assayed for cross reactivity. No cross reactivity or interference was observed.
Datasheets and documents
ab184860 has been referenced in 3 publications.
- Yang Y & Min Z Effect of long non-coding RNA AK021443 on promoting hepatic fibrosis in vitro. Mol Med Rep 23:N/A (2021). PubMed: 33495828
- Wakayama T et al. Quality comparison between two different types of platelet-rich plasma for knee osteoarthritis. Regen Med Res 8:3 (2020). PubMed: 33287956
- Pirklbauer M et al. Unraveling reno-protective effects of SGLT2 inhibition in human proximal tubular cells. Am J Physiol Renal Physiol 316:F449-F462 (2019). PubMed: 30539648