Human Periostin ELISA Kit (ab309314)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 20.98 pg/ml
- Range: 78.125 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Periostin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.8% Inter-assay Sample n Mean SD CV% Serum 3 1.6% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
20.98 pg/ml -
Range
78.125 pg/ml - 5000 pg/ml -
Recovery
90 %
Sample specific recovery Sample type Average % Range Serum 90 87% - 92% Cell culture media 109 101% - 123% Hep Plasma 99 91% - 111% EDTA Plasma 87 78% - 95% Cit plasma 97 89% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Periostin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Periostin protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Periostin with 20.98 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Periostin Capture Antibody 1 x 600µl 10X Human Periostin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI2 1 x 6ml Human Periostin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Function
Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
Tissue specificity
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Golgi apparatus. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi. - Information by UniProt
-
Alternative names
- OSF-2
- Osteoblast-specific factor 2
- Periostin
see all -
Database links
- Entrez Gene: 10631 Human
- SwissProt: Q15063 Human
- Unigene: 136348 Human
Images
-
Example of human Periostin standard curve in Sample Diluent NS.
Example of human Periostin standard curve. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of human Periostin in serum, plasma (citrate), plasma (heparin), and plasma (EDTA).Interpolated concentration of native Periostin was measured in duplicate at different sample concentrations. Undiluted samples are 1:80 serum, plasma (citrate), plasma (heparin), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
-
Interpolated concentrations of Periostin in normal human serum donors.Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Periostin concentration was determined to be 123.81 ng/mL with a range of 47.55 – 200.71 ng/mL.
-
Human Periostin ELISA KitSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Human Periostin ELISA Kit -
Sandwich ELISA - Human Periostin ELISA Kit (ab309314)To learn more about the advantages of SimpleStep ELISA® kits see here.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab309314 has not yet been referenced specifically in any publications.