For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/elisa/human-periostin-elisa-kit-osf2-ab213816.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Focal Adhesions
Share by email

Human Periostin ELISA Kit (OSF2) (ab213816)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 156 pg/ml - 10000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    You may also be interested in

    ELISA
    Product image
    Human MMP13 ELISA Kit (ab100605)
    Protein
    Product image
    Recombinant Human Periostin protein (ab203522)
    Pair
    Product image
    Human IL-1beta Antibody Pair - BSA and Azide free (ab241807)

    View more associated products

    Overview

    • Product name

      Human Periostin ELISA Kit (OSF2)
      See all Periostin kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      1 16 188pg/ml 8.64 = 4.6%
      2 16 1707pg/ml 124.61 = 7.3%
      3 16 4824pg/ml 192.96 = 4%
      Inter-assay
      Sample n Mean SD CV%
      1 24 180pg/ml 11.34 = 8.2%
      2 24 1664pg/ml 136.44 = 8.2%
      3 24 4867pg/ml 199.54 = 4.1%
    • Sample type

      Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 10 pg/ml
    • Range

      156 pg/ml - 10000 pg/ml
    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      The Human Periostin Enzyme-Linked Immunosorbent Assay (ELISA) kit (OSF2) (ab213816) is designed for the quantitative measurement of Human Periostin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).


      The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Periostin/OSF2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: N22-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin/OSF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Periostin/OSF2 amount of sample captured in plate.

    • Notes

      Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was over-expressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Human Periostin coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti-Human Periostin antibody 1 x 100µl
      Lyophilized recombinant Human Periostin standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • Structures
      • Focal Adhesions
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • Structures
      • Bone
      • Tags & Cell Markers
      • Cell Type Markers
      • Other Cell Types
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • Other
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Cell adhesion molecules
      • Cardiovascular
      • Heart
      • Cardiogenesis
      • Cardiomyocyte precursors
    • Function

      Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
    • Tissue specificity

      Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
    • Sequence similarities

      Contains 1 EMI domain.
      Contains 4 FAS1 domains.
    • Post-translational
      modifications

      Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession Q15063 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Fasciclin-I like
      • MGC119510
      • MGC119511
      • OSF 2
      • OSF-2
      • OSF2
      • Osteoblast specific factor
      • Osteoblast specific factor 2
      • Osteoblast specific factor 2 (fasciclin I like)
      • Osteoblast-specific factor 2
      • PDLPOSTN
      • Periodontal ligament specific periostin
      • Periostin
      • Periostin isoform thy2
      • Periostin isoform thy4
      • Periostin isoform thy6
      • Periostin isoform thy8
      • Periostin osteoblast specific factor
      • PN
      • POSTN
      • POSTN_HUMAN
      • RP11 412K4.1
      see all
    • Database links

      • Entrez Gene: 10631 Human
      • Omim: 608777 Human
      • SwissProt: Q15063 Human
      • Unigene: 136348 Human
      • Unigene: 721018 Human

      Images

      • Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.
        Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.

        Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab213816? Please let us know so that we can cite the reference in this datasheet.

      ab213816 has been referenced in 1 publication.

      • Qaisar R  et al. Reduced sarcoplasmic reticulum Ca2+ ATPase activity underlies skeletal muscle wasting in asthma. Life Sci 273:119296 (2021). PubMed: 33675897

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab213816.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2023 Abcam plc. All rights reserved.