Human Periostin ELISA Kit (OSF2) (ab213816)

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Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 156 pg/ml - 10000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    Overview

    • Product name

      Human Periostin ELISA Kit (OSF2)
      See all Periostin kits

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      1 16 188pg/ml 8.64 = 4.6%
      2 16 1707pg/ml 124.61 = 7.3%
      3 16 4824pg/ml 192.96 = 4%
      Inter-assay
      Sample n Mean SD CV%
      1 24 180pg/ml 11.34 = 8.2%
      2 24 1664pg/ml 136.44 = 8.2%
      3 24 4867pg/ml 199.54 = 4.1%

    • Sample type

      Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 10 pg/ml

    • Range

      156 pg/ml - 10000 pg/ml

    • Assay time

      3h 30m

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Human

    • Product overview

      The Human Periostin Enzyme-Linked Immunosorbent Assay (ELISA) kit (OSF2) (ab213816) is designed for the quantitative measurement of Human Periostin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).


      The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Periostin/OSF2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: N22-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin/OSF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Periostin/OSF2 amount of sample captured in plate.

    • Notes

      Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was over-expressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    Images

    • Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.
      Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.

      Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.

    References (1)

    Publishing research using ab213816? Please let us know so that we can cite the reference in this datasheet.

    ab213816 has been referenced in 1 publication.

    • Qaisar R  et al. Reduced sarcoplasmic reticulum Ca2+ ATPase activity underlies skeletal muscle wasting in asthma. Life Sci 273:119296 (2021). PubMed: 33675897

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