Human Periostin ELISA Kit (OSF2) (ab213816)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Periostin ELISA Kit (OSF2)
See all Periostin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 188pg/ml 8.64 = 4.6% 2 16 1707pg/ml 124.61 = 7.3% 3 16 4824pg/ml 192.96 = 4% Inter-assay Sample n Mean SD CV% 1 24 180pg/ml 11.34 = 8.2% 2 24 1664pg/ml 136.44 = 8.2% 3 24 4867pg/ml 199.54 = 4.1% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Periostin Enzyme-Linked Immunosorbent Assay (ELISA) kit (OSF2) (ab213816) is designed for the quantitative measurement of Human Periostin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Periostin/OSF2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: N22-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin/OSF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Periostin/OSF2 amount of sample captured in plate.
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Notes
Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was over-expressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Periostin coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human Periostin antibody 1 x 100µl Lyophilized recombinant Human Periostin standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
Tissue specificity
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Fasciclin-I like
- MGC119510
- MGC119511
see all -
Database links
- Entrez Gene: 10631 Human
- Omim: 608777 Human
- SwissProt: Q15063 Human
- Unigene: 136348 Human
- Unigene: 721018 Human
Images
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Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.
Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab213816 has been referenced in 1 publication.
- Qaisar R et al. Reduced sarcoplasmic reticulum Ca2+ ATPase activity underlies skeletal muscle wasting in asthma. Life Sci 273:119296 (2021). PubMed: 33675897