Human PF4 ELISA Kit (CXCL4) (ab189573)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24.29 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman PF4 ELISA Kit (CXCL4)
See all PF4 kits
Intra-assay Sample n Mean SD CV% Overall 8 3.7% Inter-assay Sample n Mean SD CV% Overall 3 3.5%
Sample typeCell culture supernatant, Serum, Cell culture media, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range78.13 pg/ml - 5000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 108 100% - 115% Serum 93 89% - 99% Cell culture media 87 85% - 90% EDTA Plasma 103 97% - 115% Cit plasma 92 83% - 100%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
As of November 23, 2020, Human PF4 (CXCL4) ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human PF4 ELISA Kit (CXCL4) (ab189573) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PF4 (CXCL4) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PF4 (CXCL4) with 24.29 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Platelet factor-4 (PF4/CXCL4) is a 70-amino acid protein that is released from the alpha-granules of activated platelets whose major physiologic role appears to be inhibiting local antithrombin III activity and promoting coagulation. PF4 (CXCL4) has a role in inflammation and wound repair by acting as a strong chemoattractant for neutrophils, monocytes, and fibroblasts.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human PF4 Capture Antibody 1 x 600µl 10X Human PF4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human PF4 Lyophilized Recombinant Protein (ab9562) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionReleased during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsBinds non-covalently to a proteoglycan molecule.
- Information by UniProt
- C-X-C motif chemokine 4
- Chemokine (C X C motif) ligand 4
- Chemokine (CXC motif) ligand 4
- Entrez Gene: 5196 Human
- Omim: 173460 Human
- SwissProt: P02776 Human
- Unigene: 81564 Human
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of PF4 were measured in duplicates, interpolated from the PF4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2%, plasma (citrate) 0.5%, and plasma (EDTA) 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 249.53 ng/mL in serum, 622.09 ng/mL in plasma (citrate), and 117.28 ng/mL in plasma (EDTA).
The concentrations of PF4 were measured in duplicates, interpolated from the PF4 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 19.25 ng/mL in PBMC cell culture supernatant.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 267.15 ng/mL with a range of 47.05 – 986.18 ng/mL.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PF4 concentration was determined to be 104.38 ng/mL with a range of 27.73 – 353.63 ng/mL.
To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
ab189573 has been referenced in 5 publications.
- Hernáez Á et al. Mediterranean Diet and Atherothrombosis Biomarkers: A Randomized Controlled Trial. Mol Nutr Food Res 64:e2000350 (2020). PubMed: 32918853
- Huang K & Ge S The anti-CXCL4 antibody depletes CD4(+)CD25(+)FOXP3(+) regulatory T cells in CD4+ T cells from chronic osteomyelitis patients by the STAT5 pathway. Ann Palliat Med 9:2723-2730 (2020). PubMed: 32954739
- Thomas KA et al. Effect of leukoreduction and pathogen reduction on the hemostatic function of whole blood. Transfusion 59:1539-1548 (2019). PubMed: 30980757
- Denis HL et al. Platelet abnormalities in Huntington's disease. J Neurol Neurosurg Psychiatry 90:272-283 (2019). PubMed: 30567722
- Alsmadi NZ et al. Constricted microfluidic devices to study the effects of transient high shear exposure on platelets. Biomicrofluidics 11:064105 (2017). PubMed: 29204246