Human PGC ELISA Kit (ab275552)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 16.5 pg/ml
- Range: 54.69 pg/ml - 3500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human PGC ELISA Kit
See all Pepsinogen II/PGC kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.8% Inter-assay Sample n Mean SD CV% Serum 3 8.1% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16.5 pg/ml -
Range
54.69 pg/ml - 3500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 104 99% - 108% Hep Plasma 95 93% - 98% EDTA Plasma 101 93% - 107% Cit plasma 90 88% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PGC ELISA kit (ab275552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PGC protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PGC with 16.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Pepsinogen C (PGC, progastricsin) is an aspartic proteinase zymogen that belongs to the peptidase family A1. Pespsin C, the activated protease, is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. Pepsinogen C protein is synthesized as an inactive zymogen that autocatalizes by sequential cleavage of the prosegment into its active mature form at low pH. Pepsinogen C polymorphisms are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PGC (Pepsinogen C) Capture Antibody 1 x 600µl 10X Human PGC (Pepsinogen C) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human PGC (Pepsinogen C) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Hydrolyzes a variety of proteins. -
Sequence similarities
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Gastricsin
- PEPC
- PEPC_HUMAN
see all -
Database links
- Entrez Gene: 5225 Human
- Omim: 169740 Human
- SwissProt: P20142 Human
- Unigene: 1867 Human
Associated products
-
Alternative Versions
Images
-
Sandwich ELISA - Human PGC ELISA Kit (ab275552)To learn more about the advantages of SimpleStep ELISA® kits see here.
-
SimpleStep ELISA Protocol DiagramSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human PGC standard curve in Sample Diluent NS.The PGC standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native PGC in human serum and plasma samples.The concentrations of PGC were measured in duplicates, interpolated from the PGC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 50%, and plasma (heparin) 25% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PGC concentration was determined to be 12.02 ng/mL in serum, 8.96 ng/mL in plasma (citrate), 6.89 ng/mL in and plasma (EDTA), and 8.41 ng/mL in plasma (heparin).
-
Sandwich ELISA - Human PGC ELISA Kit (ab275552)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab275552 has been referenced in 1 publication.
- Tang Y et al. A panel of urine-derived biomarkers to identify sepsis and distinguish it from systemic inflammatory response syndrome. Sci Rep 11:20794 (2021). PubMed: 34675320