Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit (ab216064)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit
See all Procollagen Type 1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
8.44 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Pro-Collagen I alpha 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Pro-Collagen I alpha 1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210966. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 5 x 96 tests Human Pro-Collagen I alpha 1 Capture Antibody 1 x 50µg Human Pro-Collagen I alpha 1 Detector Antibody 1 x 12.5µg Human Pro-Collagen I alpha 1 Lyophilized Protein 1 vial -
Research areas
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Function
Type I collagen is a member of group I collagen (fibrillar forming collagen). -
Tissue specificity
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. -
Involvement in disease
Caffey disease
Ehlers-Danlos syndrome, classic type
Ehlers-Danlos syndrome 7A
Osteogenesis imperfecta 1
Osteogenesis imperfecta 2
Osteogenesis imperfecta 3
Osteogenesis imperfecta 4
Osteoporosis
A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. -
Sequence similarities
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. -
Domain
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function. -
Post-translational
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Alternative names
- Alpha 1 type I collagen
- Alpha 2 type I collagen
- Alpha-1 type I collagen
see all -
Database links
- Entrez Gene: 1278 Human
- Entrez Gene: 1277 Human
- SwissProt: P08123 Human
- SwissProt: P02452 Human
- Unigene: 172928 Human
- Unigene: 681002 Human
Associated products
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Alternative Versions
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Related Products
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SimpleStep ELISA kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
Images
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Human Pro-Collagen I alpha 1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit (ab216064)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab216064 has been referenced in 1 publication.
- van Vijven M et al. Identifying potential patient-specific predictors for anterior cruciate ligament reconstruction outcome - a diagnostic in vitro tissue remodeling platform. J Exp Orthop 7:48 (2020). PubMed: 32623555