Human RANTES ELISA Kit (CCL5) (ab174446)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.091 pg/ml
- Range: 0.94 pg/ml - 60 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman RANTES ELISA Kit (CCL5)
See all RANTES kits
Intra-assay Sample n Mean SD CV% Serum 8 5% Inter-assay Sample n Mean SD CV% Serum 3 2.8%
Sample typeCell culture supernatant, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range0.94 pg/ml - 60 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 95% - 114% Serum 107 77% - 132% Hep Plasma 91 79% - 100% EDTA Plasma 105 81% - 119% Cit plasma 105 82% - 124%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human RANTES ELISA kit (CCL5) (ab174446) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANTES protein in human plasma, serum and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human RANTES with 0.091 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
RANTES (Regulated on Activation, Normal T cell Expressed and Secreted) is a chemotactic cytokine for T cells, eosinophils, and basophils. RANTES also acts as a recruitment signal for leukocytes into inflammatory sites with the help of cyotokines (IL-2 and IFN-γ) released by CD8+ T cells. RANTES also activates and induces proliferation of natural-killer cells to form CHAK (CC-Chemokine-activated killer) cells. RANTES has also been identified to inhibit infection of HIV.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human RANTES Capture Antibody 1 x 600µl 10X Human RANTES Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Human RANTES Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
Tissue specificityT-cell and macrophage specific.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsN-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
- Information by UniProt
- Beta chemokine RANTES
- Beta chemokine RANTES precursor
- C C motif chemokine 5
- Entrez Gene: 6352 Human
- Omim: 187011 Human
- SwissProt: P13501 Human
- Unigene: 514821 Human
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Standard Curve comparison between human RANTES SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
The RANTES standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 0.63%, plasma (heparin) 2.5% and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 2,141 pg/mL in neat serum, 13,050 pg/mL in plasma (citrate) and 3,157 pg/mL in plasma (heparin) and 4,457 pg/mL plasma (EDTA).
The supernatant was decanted and assayed. The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 2,156 pg/mL in neat PBMC supernatant.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 977 pg/mL with a range of 135–2,378 pg/mL.
PBMCs were stimulated with 1.5% PHA-M for 36 hours. The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 2,156 pg/mL in neat PBMC stimulated supernatant and non-detectable in unstimulated supernatant.
To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
ab174446 has been referenced in 7 publications.
- Jia J et al. Cutaneous neurofibroma cells with active YAP promotes proliferation of macrophages resulting in increased accumulation of macrophages by modulating CCL5 and TGF-ß1. Oncol Rep 43:1319-1330 (2020). PubMed: 32323813
- Li S et al. Clinical and pathological investigation of patients with severe COVID-19. JCI Insight 5:N/A (2020). PubMed: 32427582
- Hosseinkhani B et al. (Sub)populations of extracellular vesicles released by TNF-a -triggered human endothelial cells promote vascular inflammation and monocyte migration. J Extracell Vesicles 9:1801153 (2020). PubMed: 32944190
- Hosseinkhani B et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). PubMed: 30131806
- Aznar MA et al. CD137 (4-1BB) Costimulation Modifies DNA Methylation in CD8+ T Cell-Relevant Genes. Cancer Immunol Res 6:69-78 (2018). PubMed: 29133290
- Li BH et al. Steatosis induced CCL5 contributes to early-stage liver fibrosis in nonalcoholic fatty liver disease progress. Transl Res 180:103-117.e4 (2017). Sandwich ELISA ; Human . PubMed: 27639593
- Lai XM et al. HAF mediates the evasive resistance of anti-angiogenesis TKI through disrupting HIF-1a and HIF-2a balance in renal cell carcinoma. Oncotarget 8:49713-49724 (2017). Sandwich ELISA ; Human . PubMed: 28572533