Human Resistin ELISA Kit (ab183364)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24 pg/ml
- Range: 78.1 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Resistin ELISA Kit
See all Resistin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 9 3% Inter-assay Sample n Mean SD CV% Serum 3 6% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
24 pg/ml -
Range
78.1 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94 88% - 100% Cell culture media 106 106% - 107% Hep Plasma 95 84% - 110% EDTA Plasma 96 88% - 104% Cit plasma 78 68% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Resistin ELISA kit (ab183364) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Resistin protein in human serum, plasma and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Resistin with 24 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
Notes
Resistin, otherwise known as Found in Inflammatory Zone 3 (FIZZ3), and Adipocyte Secreted Factor (ADSF), is a 114 amino-acid cysteine-rich protein. It can be found as a high molecular weight hexamer or as a more active, lower molecular weight complex. Resistin is the best known member of the family of Resistin-like Molecules (RELMs), which include RELM-alpha/FIZZ1 and RELM-beta/FIZZE 2, both described only in rodents. Furthermore, resistin has been categorized as part of the group of adipokines hormones, which are known to be involved in the development of obesity, insulin resistance, diabetes, inflammation, autoimmune disease and metabolic syndrome.
In Humans, Resistin is a pro-inflammatory molecule primarily secreted by macrophages and mononuclear bone marrow cells in response to inflammation, hyperglycemia, and growth hormones. IL-1, IL-6, TNF-alpha as well as lipopolysaccharide (LPS) are strong inducers of resistin as observed in animal models of hepatic inflammation and necrosis. High levels of resistin are also observed in patients with pancreatitis and with systemic lupus erythematosus.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Resistin Capture Antibody 1 x 600µl 10X Human Resistin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Resistin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
Tissue specificity
Expressed only in fatty tissues. -
Sequence similarities
Belongs to the resistin/FIZZ family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all -
Database links
- Entrez Gene: 56729 Human
- Omim: 605565 Human
- SwissProt: Q9HD89 Human
- Unigene: 283091 Human
Associated products
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ELISA kits
Images
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Other - Human Resistin ELISA Kit (ab183364)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Resistin standard curve.Background-subtracted data values (mean +/- SD) are graphed
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Levels of Resistin in Normal Serum.The levels of native Resistin in serum samples were tested from ten individual healthy donors. Levels were interpolated from the standard curve in Sample Diluent NS. The mean level of Resistin was 2.6 ng/mL with a range of 1.6 to 5.3 ng/mL and a standard deviation of 1 ng/mL.
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Example of Resistin standard curve in cell culture media.Background-subtracted data values (mean +/- SD) are graphed.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183364 has not yet been referenced specifically in any publications.