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SimpleStep

Human Thioredoxin Reductase 1 ELISA Kit (ab192150)

  • Datasheet
  • SDS
  • Protocol Booklet
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Other - Human Thioredoxin Reductase 1 ELISA Kit (ab192150)
  • Example of Thioredoxin Reductase 1 standard curve.
  • The assay is shown to not react with recombinant Human Thioredoxin Reductase 2.
  • Titration of HeLa and HepG2 extracts within the working range of the assay.

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.19 ng/ml
  • Range: 0.39 ng/ml - 25 ng/ml
  • Sample type: Cell culture extracts, Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human Thioredoxin Reductase 1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HeLa extract 4 3.77%
    Inter-assay
    Sample n Mean SD CV%
    HeLa extract 4 7.3%
  • Sample type

    Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.19 ng/ml
  • Range

    0.39 ng/ml - 25 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 101 100% - 102%
    Cell culture media 98 89% - 106%
    Hep Plasma 101 100% - 102%
    EDTA Plasma 104 98% - 115%
    Cit plasma 99 97% - 101%
    Extraction Buffer 96 84% - 108%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Thioredoxin Reductase 1 ELISA Kit (ab192150) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thioredoxin Reductase 1 protein in serum, tissue extracts, plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thioredoxin Reductase 1 with 0.14 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Thioredoxin Reductase 1 (TXNRD1) is a selenium-containing enzyme part of the thioredoxin system responsible for regulating oxidative stress and redox signaling via reduction of disulfide bonds. The dimer, TXNRD1, reduces thioredoxin and other substrates using NADPH and an FAD cofactor. TXNRD1’s involvement in protecting against oxidative stress and injury, regulation of cellular development and growth, and various other cellular processes make it an interesting target for studies of various cancers, AIDS, and other diseases. Humans express three different isozymes of thioredoxin reductase, isoform 1 is the cytosolic form, isoform 2 is the mitochondrial form, and isoform 3 is a testes specific form. This assay is specific to isoform 1 of thioredoxin reductase.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    10X Human TXNRD1 Capture Antibody 1 x 600µl
    10X Human TXNRD1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human TXNRD1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Alternative names

    • Gene associated with retinoic and IFN induced mortality 12 protein
    • Gene associated with retinoic and interferon induced mortality 12 protein
    • GRIM 12
    • KM 102 derived reductase like factor
    • Oxidoreductase
    • Thioredoxin reductase 1
    • Thioredoxin reductase 1 cytoplasmic
    • Thioredoxin reductase GRIM 12
    • Thioredoxin reductase TR1
    • TR
    • TR1
    • TRXR1
    • TXNR
    see all
  • Database links

    • Entrez Gene: 7296 Human
    • Omim: 601112 Human
    • SwissProt: Q16881 Human

    Images

    • Other - Human Thioredoxin Reductase 1 ELISA Kit (ab192150)
      Other - Human Thioredoxin Reductase 1 ELISA Kit (ab192150)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of Thioredoxin Reductase 1 standard curve.
      Example of Thioredoxin Reductase 1 standard curve.

      Background-subtracted data values (mean +/- SD) are graphed.

    • The assay is shown to not react with recombinant Human Thioredoxin Reductase 2.
      The assay is shown to not react with recombinant Human Thioredoxin Reductase 2.
    • Titration of HeLa and HepG2 extracts within the working range of the assay.
      Titration of HeLa and HepG2 extracts within the working range of the assay.

      Background-subtracted data values (mean +/- SD, n = 2) are graphed.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab192150? Please let us know so that we can cite the reference in this datasheet.

    ab192150 has been referenced in 2 publications.

    • Paul S  et al. Plasma protein(s)-based conceptual diagnostic tool for assessing high-altitude acclimation in humans. Funct Integr Genomics 20:191-200 (2020). PubMed: 31444657
    • Cecchinato V  et al. Redox-Mediated Mechanisms Fuel Monocyte Responses to CXCL12/HMGB1 in Active Rheumatoid Arthritis. Front Immunol 9:2118 (2018). PubMed: 30283452

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