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  1. Link

    products/elisa/human-thrombin-antithrombin-complex-elisa-kit-tat-ab108907.pdf

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Cardiovascular Blood Fibrinolysis / Thrombolysis
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Human Thrombin-Antithrombin Complex ELISA Kit (TAT) (ab108907)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (3)Q&A (14)References (16)

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Sandwich ELISA - Thrombin-Antithrombin Complex (TAT) Human ELISA Kit
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.78 ng/ml
  • Range: 1 ng/ml - 10 ng/ml
  • Sample type: Cell culture supernatant, Milk, Plasma
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human Thrombin-Antithrombin Complex ELISA Kit (TAT)
    See all Thrombin-Antithrombin Complex kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10.4%
  • Sample type

    Cell culture supernatant, Milk, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.78 ng/ml
  • Range

    1 ng/ml - 10 ng/ml
  • Recovery

    96 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Thrombin-Antithrombin Complex (TAT) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin-Antithrombin Complex concentrations in plasma, milk and cell culture supernatants.


    A Thrombin-Antithrombin Complex specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin-Antithrombin Complex specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin-Antithrombin Complex captured in plate.


    Note: It is not recommended to perform spike recovery in plasma samples due to large amount of endogenous Anti-thrombin III (mg/ml range) which will saturate the capture antibody and interfere with the binding of the TAT standard


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Thrombin-Antithrombin Complex Antibody 1 x 120µl
    Chromogen Substrate 1 x 7ml
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml
    Thrombin-Antithrombin Complex Microplate (12 x 8 well strips) 1 unit
    Thrombin-Antithrombin Complex Standard 1 vial
  • Research areas

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cardiovascular
    • Blood
    • Coagulation
    • Regulatory
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cardiovascular ELISA kits
  • Relevance

    Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Cellular localization

    Secreted
  • Alternative names

    • antithrombin III
    • AT3
    • AT3D
    • ATIII
    • coagulation factor II (thrombin)
    • F2
    • PRO0309
    • prothrombin
    • prothrombin B chain
    • PT
    • RPRGL2
    • serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
    • serine protease
    • serine-cysteine proteinase inhibitor clade C member 1
    • serpin C1
    • serpin peptidase inhibitor, clade C (antithrombin), member 1
    • SERPINC1
    • TAT
    • THPH1
    • THPH7
    see all
  • Database links

    • Entrez Gene: 462 Human
    • Entrez Gene: 2147 Human
    • Omim: 107300 Human
    • Omim: 176930 Human
    • SwissProt: P01008 Human
    • SwissProt: P00734 Human

    Images

    • Sandwich ELISA - Thrombin-Antithrombin Complex (TAT) Human ELISA Kit
      Sandwich ELISA - Thrombin-Antithrombin Complex (TAT) Human ELISA Kit

      TAT measured in biological fluids, with background signal subtracted (duplicates +/- SD).

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve Using ab108907.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (16)

    Publishing research using ab108907? Please let us know so that we can cite the reference in this datasheet.

    ab108907 has been referenced in 16 publications.

    • Noguchi D  et al. The Impact of Dabigatran Treatment on Sinusoidal Protection Against Hepatic Ischemia/Reperfusion Injury in Mice. Liver Transpl 27:363-384 (2021). PubMed: 33108682
    • Pang M  et al. The significance of coagulation and fibrinolysis-related parameters in predicting postoperative venous thrombosis in patients with breast cancer. Gland Surg 10:1439-1446 (2021). PubMed: 33968695
    • Zhang H  et al. Tissue Factor-Enriched Neutrophil Extracellular Traps Promote Immunothrombosis and Disease Progression in Sepsis-Induced Lung Injury. Front Cell Infect Microbiol 11:677902 (2021). PubMed: 34336711
    • Sommerfeld O  et al. Complement factor D is linked to platelet activation in human and rodent sepsis. Intensive Care Med Exp 9:41 (2021). PubMed: 34396466
    • Tang X  et al. Transferrin plays a central role in coagulation balance by interacting with clotting factors. Cell Res 30:119-132 (2020). PubMed: 31811276
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 17 Abreviews or Q&A

    Human Thrombin-Antithrombin detection

    Good Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    Detection o fThrombin-Antithrombin complex in human citrated plasma samples
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 20 2021

    Testing Crossreactivity with NHP EDTA plasma

    Good Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Human Thrombin-Antithrombin Complex ELISA Kit Abcam Review:

    An experienced laboratory technician completed this Thrombin-Antithrombin ELISA from Abcam, catalog number AB108907, Lot GR256365-9. The goal of the experiment was to analyze the linearity of dilutions of 3 different rhesus macaque EDTA-prepared plasma samples, but more specifically, to analyze the cross-reactivity for rhesus macaque.

    Overall, the kit, like other Abcam ELISA kits, was easy to follow, and was straight-forward in directions, while also being brief. The laboratory technician had no problem understanding any parts of the protocol.

    Our experiment consisted of using EDTA-prepared rhesus macaque plasma samples from N=3 M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius. Plasma was diluted 1:5, 1:10, 1:20, and 1:40 in the kit’s diluent, and tested in duplicate, as were the standards. The rest of the assay was performed to protocol’s specifications.

    The kit recommended 5 standard dilutions. We noted that the highest concentration all but reaches 1.0 OD when assay recommendations are followed. We recommend using a higher-sensitivity TMB substrate or extending TMB incubation time considerably.

    The tested sample dilutions all had blank-subtracted OD less than 0.05. Spiked human protein was recoverable at 1:40 (60%).

    Overall, we do not believe this kit is capable of detecting TAT complexes in plasma of rhesus macaque.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 06 2016

    Thrombin-anti-Thrombin ELISA in NHP (Rhesus Macaque)

    Good Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Stored serum samples (-20) were used from rhesus macaque monkeys (NHP), 1:5 sample dilution with Abcam TAT ELISA kit used as per protocol to measure Thrombin-Anti-Thrombin complexes post-transplant, see attached image.
    Colour development was faster (3-5m) than the c.20 mins anticipated in the protocol.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 02 2015

    Question

    I was wondering if you could give me an expiration date for the TAT kits (ab108907)

    Read More

    Abcam community

    Verified customer

    Asked on Feb 25 2013

    Answer



    All our kits are guaranteed for the first six months. But having said that, stored at the right conditions, the reagents could last up to a year.

    Read More

    Abcam Scientific Support

    Answered on Feb 25 2013

    Question

    How should the standard be diluted to produce the standard curve?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer





    TAT Complexes Standard: Reconstitute the 240 ng of human TAT Complexes Standard with 2 ml of MIX Diluent to generate a standard solution of 120 ng/ml. Allow the standard to sit for 10 minutes with gentle agitation prior to making dilutions. Prepare duplicate or triplicate standard points by serially diluting the Standard solution 1:3 with MIX Diluent to produce 40, 13.33, 4.44, and 1.48 ng/ml. MIX Diluent serves as the zero standard




    (0 ng/ml). Any remaining solution should be frozen at -200C. Standard Point
    Dilution
    [TAT] (ng/ml)

    P1
    1 part Standard Solution (120 ng/ml)
    120.00

    P2
    1 part P1 + 2 parts MIX Diluent
    40.00

    P3
    1 part P2 + 2 parts MIX Diluent
    13.33

    P4
    1 part P3 + 2 parts MIX Diluent
    4.44

    P5
    1 part P4 + 2 parts MIX Diluent
    1.48

    P6
    MIX Dilutent
    0.00

    Read More

    Abcam Scientific Support

    Answered on Dec 10 2012

    Question

    What is the catalogue number of Thrombin-Antithrombin Complex (TAT) Human ELISA Kit?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 04 2012

    Answer

    Thank you for contacting us.

    The catalogue number is ab108907

    https://www.abcam.com/Thrombin-Antithrombin-Complex-TAT-Human-ELISA-Kit-ab108907.html

    Please find attached the datasheet.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 04 2012

    Question

    Hi tech support!

    Would it be possible for you to help one of our customers out with reference to the following question please:

    We purchased the Thrombin-Antithrombin Complex (TAT) Human ELISA Kit (ab108907) and was unable to find the normal reference range in the booklet provided nor the website. Could you please provide us with the normal reference range for this ELISA Kit. We ran the kit with EDTA samples.

    Looking forward to your response!

    Read More

    Abcam community

    Verified customer

    Asked on Jul 02 2012

    Answer

    Thank you for contacting us.

    I am sorry you could not find this information in the protocol book. The range for this kit is stated on our datasheet and corresponds to 0.48 ng/ml - 120 ng/ml.

    I hope this helps and if we can assist further, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Jul 02 2012

    Question

    We purchased the Thrombin-Antithrombin Complex (TAT) Human ELISA Kit (ab108907) and was unable to find the normal reference range in the booklet provided nor the website. Could you please provide us with the normal reference range for this ELISA Kit. We ran the kit with EDTA samples.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 29 2012

    Answer


    The range for the strandard is from 1.48 to 120 ng/ml, as shown on pages 8 and 9 of the booklet: https://www.abcam.com/ps/products/108/ab108907/documents/ab108907%20Thrombin%20Antithrombin%20Complex%20Human%20ELISA%20Kit%20(Web)%20v2.pdf.

    I hope this information is the answer toyour question. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jun 29 2012

    Question

    Hello, I would like to know if your thrombin-antithrombin complex ELISA kit, catalogue # ab108907, can be used for mouse plasma. Thank you in advance.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 26 2012

    Answer

    Thank you for contacting Abcam.
    Unfortunately ab108907 will not work well with mouse samples. We do have a few other kits that work on mouse that may be suitable though:
    https://www.abcam.com/Antithrombin-III-Mouse-ELISA-Kit-ab108800.html
    https://www.abcam.com/Antithrombin-III-Human-ELISA-Kit-ab108801.html

    If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Jun 26 2012

    Question

    We tried the TAT kit today doing a half-plate (6 strips), and this looks fine for a first attempt – Standard curve attached. All seems very straightforward to use.
    We will run the other half tomorrow.
    I have frozen the remaining stock standard and biotinylated antibody for tomorrow as only one of each is supplied – I hope this was the right thing to do?
    So that we can complete our samples for the current project, I have placed an order for one more kit this afternoon. Hopefully you have one in stock?

    Best wishes

    Read More

    Abcam community

    Verified customer

    Asked on May 01 2012

    Answer

    Thank you for your reply which has been forwarded to me as my colleague Karin is currently away from the office.

    I am pleased to hear the kit has worked well for you. In answer to your questions:

    1. Yes, as stated in the protocol the standard and biotinylated antibody can be kept at -20oC once reconstituted. Although I would recommend to avoid freeze thaw cycles as much as possible (eg by aliquotting).

    2. I can confirm we have stock at the moment.

    I hope this will be helpful to you. Good luck with your experiments and please do not hesitate to contact us if you require anything further.

    Read More

    Abcam Scientific Support

    Answered on May 01 2012

    1-10 of 17 Abreviews or Q&A

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